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Biosensors
Special Issues
Nanobiosensors and Immunoassay
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Nanobiosensors and Immunoassay

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Nano- and Micro-Technologies in Biosensors".

Deadline for manuscript submissions: closed (30 December 2022) | Viewed by 9266

Special Issue Editors

Prof. Dr. Xu Yan

E-Mail Website
Guest Editor
College of Electronic Science & Engineering, Jilin University, Changchun 130015, China
Interests: bio/immune-sensors; optical sensor; electrochemical sensor; public safety
Prof. Dr. Changlong Jiang

E-Mail Website
Guest Editor
State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Hefei 230031, China
Interests: nanomaterials; point-of-care testing; wearable equipment
Dr. Hongxia Li

E-Mail Website
Guest Editor
Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, Changchun 130062, China
Interests: agro-sensor; food safety; environment monitoring

Special Issue Information

Dear Colleagues,

The main topic of this Special Issue is the development of biosensors and immunosensors. In this regard, this Special Issue aims to collect original articles that present research advances in the design, fabrication, and application of high-performance (bio)sensors and immunosensors that implement new principles, strategies, methods, and technologies. Additionally, reviews reflecting the current hotspots, new challenges, and future perspectives of bio/immune-sensors in the important application areas are particularly welcome.

Nanobiosensors and immunosensors are considered to be efficient tools for a wide variety of analytical problems, including uses in medicine, biomedical research, drug discovery, the environment, food, process industries, security and defence. In this regard, interesting principles, strategies, and methods are being proposed to design, develop, and fabricate bio-/immune-sensors. Moreover, bio-/immune-sensors based on various sensing modes (optical, electrochemical, photoelectric, thermometric, piezoelectric, magnetic or micromechanical, etc.) and using various recognition elements (enzymes, DNA/aptamers, antibodies/antigens, molecularly imprinted polymers, etc.) are satisfying the growing needs of methods and devices for the rapid, reliable, and high-performance detection of targets (biomarkers, toxic ions, pesticides, veterinary drugs, pathogenic bacteria, antibiotics, toxins, organic pollutants, food additives, etc.).

Prof. Dr. Xu Yan
Prof. Dr. Changlong Jiang
Dr. Hongxia Li
Guest Editors

If you want to learn more information or need any advice, you can contact the Special Issue Editor Jessica Zhou via <jessica.zhou@mdpi.com> directly.

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • nanobiosensors
  • immunoassay
  • nanomaterials
  • biomedical diagnosis
  • public safety
  • food industry
  • environment mornitoring
  • sensing platform
  • point-of-care devices
  • wearable equipment

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (3 papers)

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14 pages, 4714 KiB  
Article
Triple Enhancement for Sensitive Immunochromatographic Assay: A Case Study for Human Fatty Acid-Binding Protein Detection
by Nadezhda A. Taranova, Alisa A. Bulanaya, Anatoly V. Zherdev and Boris B. Dzantiev
Biosensors 2022, 12(12), 1166; https://doi.org/10.3390/bios12121166 - 14 Dec 2022
Viewed by 2068
Abstract
The work considers a combination of three enhancing approaches for immunochromatographic assay (ICA) and the integration of their impacts into changes of the limit of detection (LOD). Human fatty acid binding protein (FABP), an early biomarker of acute myocardial infarction, was the target [...] Read more.
The work considers a combination of three enhancing approaches for immunochromatographic assay (ICA) and the integration of their impacts into changes of the limit of detection (LOD). Human fatty acid binding protein (FABP), an early biomarker of acute myocardial infarction, was the target analyte. Starting from the common ICA protocol with an LOD equal to 11.2 ng/mL, three approaches were realized: (1) replacement of spherical gold nanoparticles with gold nanoflowers having a branched surface (20-fold lowering the LOD); (2) enhanced labeling of immune complexes via nanoparticle aggregates (15-fold lowering); (3) in-situ growth of bound nanoparticles by reduction of gold salts (3-fold lowering). Single and combined implementations of these approaches have been studied. It has been shown that the LOD decrease for combined approaches is close to the multiplied contribution of each of them. The final LOD for FABP was 0.05 ng/mL, which is 220 times lower than the LOD for the common ICA protocol. The efficiency of the enhanced ICA with three combined approaches was confirmed by testing human serum samples for FABP presence and content. The development presents a new efficient technique for rapid sensitive detection of FABP for medical diagnostics. Moreover, the demonstrated multiple enhancements could be applied for various demanded analytes. Full article
(This article belongs to the Special Issue Nanobiosensors and Immunoassay)
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15 pages, 2191 KiB  
Article
Development of an Electrochemical CCL5 Chemokine Immunoplatform for Rapid Diagnosis of Multiple Sclerosis
by Sara Guerrero, Esther Sánchez-Tirado, Lourdes Agüí, Araceli González-Cortés, Paloma Yáñez-Sedeño and José M. Pingarrón
Biosensors 2022, 12(8), 610; https://doi.org/10.3390/bios12080610 - 7 Aug 2022
Cited by 6 | Viewed by 3419
Abstract
Serum level of CCL5 chemokine is considered an emerging biomarker for multiple sclerosis (MS). Due to the lack of specific assays for this disease, the development of a point-of-care test for rapid detection of MS could lead to avoiding diagnostics delays. In this [...] Read more.
Serum level of CCL5 chemokine is considered an emerging biomarker for multiple sclerosis (MS). Due to the lack of specific assays for this disease, the development of a point-of-care test for rapid detection of MS could lead to avoiding diagnostics delays. In this paper, we report the first electrochemical immunoplatform for quantification of the CCL5 biomarker at the clinically required levels, able to discriminate between patients diagnosed with MS and healthy individuals. The immunosensing device involves protein capture from biological samples by complexation with biotinylated specific antibodies immobilized onto neutravidin-functionalized microparticles and sandwich assay with anti-CCL5 antibody and IgG labelled with horseradish peroxidase (HRP) for the enzyme-catalyzed amperometric detection of H2O2 using hydroquinone (HQ) as the redox mediator. The method shows excellent analytical performance for clinical application with a wide linear range of concentrations (0.1–300 ng·mL−1 CCL5, R2 = 0.998) and a low detection limit (40 pg·mL−1 CCL5). The biosensing platform was applied to the determination of the CCL5 endogenous content in 100-fold diluted sera both from healthy individuals and patients diagnosed with MS, with no further sample treatment in just two hours. The results were successfully compared with those obtained by the ELISA methodology. Full article
(This article belongs to the Special Issue Nanobiosensors and Immunoassay)
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12 pages, 2736 KiB  
Article
A Sensitive Monoclonal-Antibody-Based ELISA for Forchlorfenuron Residue Analysis in Food Samples
by Xinmei Liu, Bo Xie, Yongjian Cheng, Lin Luo, Yifan Liang and Zhili Xiao
Biosensors 2022, 12(2), 78; https://doi.org/10.3390/bios12020078 - 28 Jan 2022
Cited by 7 | Viewed by 2809
Abstract
In this study, forchlorfenuron (CPPU) was coupled with succinic anhydride to yield a CPPU hapten (CPPU-COOH), and a high-affinity monoclonal antibody (mAb) that can specifically recognize CPPU was produced. Using this mAb as a recognition reagent, a sensitive indirect competitive enzyme-linked immunosorbent assay [...] Read more.
In this study, forchlorfenuron (CPPU) was coupled with succinic anhydride to yield a CPPU hapten (CPPU-COOH), and a high-affinity monoclonal antibody (mAb) that can specifically recognize CPPU was produced. Using this mAb as a recognition reagent, a sensitive indirect competitive enzyme-linked immunosorbent assay (icELISA) for CPPU was optimized, which exhibits an IC50 of 1.04 ng/mL, a limit of detection of 0.16 ng/mL, and a linear range of 0.31–3.43 ng/mL for CPPU. Cross-reactivity percentages with six analogues were all below 6%. The average recovery rates for cucumber and orange samples were from 85.23% to 119.14%. The analysis results of this icELISA showed good consistency with those from liquid chromatography mass spectrometry. These results suggest that the proposed icELISA provides a sensitive, specific, and reliable strategy for CPPU detection in food samples. Full article
(This article belongs to the Special Issue Nanobiosensors and Immunoassay)
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