Optical Bioimaging and Biosensing

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Optical and Photonic Biosensors".

Deadline for manuscript submissions: closed (31 January 2025) | Viewed by 8237

Special Issue Editors


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Guest Editor
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia
Interests: optical imaging; nanomaterials
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen 518055, China
Interests: optical imaging instrumentation

Special Issue Information

Dear Colleagues,

Optical bioimaging and biosensing (e.g., fluorescence, chemiluminescence, bioluminescence, photoacoustic imaging and surface-enhanced Raman scattering imaging) has demonstrated many merits including non-ionizing radiation, high spatiotemporal resolution, good sensitivity, scalability in imaging resolution, field and depth, versatility in functionality and available imaging agents. The rapid evolution of optical instruments and technologies in microcopy, tomography, and endoscopy offer great promise for the real-time monitoring of physiological and pathological processes in molecular, cellular and animal levels, sensitively detecting biomarkers and disease microenvironment features, clearly pinpointing lesion margins, and even yielding faster responses of pharmacokinetics pharmacodynamics, and therapeutic efficacy. While a great number of optical imaging agents based on small molecules, polymers, hybrids, and nanoparticles have been customized for different application scenarios, these agents are integrated with other imaging modalities such as computed tomography (CT), magnetic resonance imaging (MRI), ultrasound imaging (US), single-photon emission computed tomography (SPECT), and positron emission tomography (PET) to offer more abundant information to boost the outcomes of bioimaging and biosensing. 

Dr. Bing Guo
Dr. Ruirui Qiao
Dr. Zhiyong Guo
Guest Editors

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Keywords

  • fluorescence imaging
  • chemiluminescence imaging
  • bioluminescence imaging
  • photoacoustic imaging
  • surface-enhanced Raman scattering imaging

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Published Papers (3 papers)

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Research

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22 pages, 3486 KiB  
Article
Development and Validation of LAMP Assays for Distinguishing MPXV Clades with Fluorescent and Colorimetric Readouts
by Nazente Atceken, Sara Asghari Dilmani, Ahmed Choukri Abdullah, Mutlu Sarıkaya, Defne Yigci, Gozde Korkmaz and Savas Tasoglu
Biosensors 2025, 15(1), 23; https://doi.org/10.3390/bios15010023 - 6 Jan 2025
Viewed by 1253
Abstract
Human monkeypox (Mpox) is a zoonotic disease caused by the Monkeypox virus (MPXV). As of 14 August 2024, the World Health Organization (WHO) has declared it a global health emergency. For Mpox, this was the second public health emergency of global significance in [...] Read more.
Human monkeypox (Mpox) is a zoonotic disease caused by the Monkeypox virus (MPXV). As of 14 August 2024, the World Health Organization (WHO) has declared it a global health emergency. For Mpox, this was the second public health emergency of global significance in the past two years. MPXV belongs to the Poxviridae family and is phylogenetically and epidemically divided into two clades: the Congo Basin (Clade-I) and the West African (Clade-II) clades. Clade-I has been associated with more severe disease progression and higher mortality compared to Clade-II, and thus the differentiation between clades can play an important role in predicting disease prognosis. The LAMP technique has the advantages of not requiring thermal cycling and achieving higher amplification in a shorter time compared to qPCR. Different types of LAMP assays were developed in this study to benefit from these advantages. We report the development of LAMP-1 and LAMP-2 assays using the LAMP method to detect MPXV Clade-I and Clade-II, respectively. The LAMP-1 assay includes both fluorescence and visible colorimetric readout tests developed with sensitivities of 103 and 107 copies, respectively. For the LAMP-2 assay, a probe-based test utilizing the Novel R-Duplex DARQ probe was developed, offering fluorescence detection at a sensitivity of 103 copies. As a result, we successfully developed three highly specific molecular diagnostic tests that distinctly differentiate between MPXV clades, delivering essential tools for the precise diagnosis and effective control of Mpox. Full article
(This article belongs to the Special Issue Optical Bioimaging and Biosensing)
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15 pages, 20696 KiB  
Article
Visualizing Macrophage Polarization through Fluorescent mRNA Profiling
by Miaomiao Xu, Siyuan Wei, Tong Su, Die Ma, Zhixuan Wang, Dan Zhu, Lixing Weng and Xianguang Ding
Biosensors 2024, 14(10), 475; https://doi.org/10.3390/bios14100475 - 2 Oct 2024
Viewed by 1603
Abstract
Macrophages, known for their phenotypic plasticity, play a critical role in maintaining homeostasis and inflammation-related pathogenesis. Although identifying diverse macrophage phenotypes holds promise for enhancing diagnoses and treatments of diseases mediated by macrophages, existing methodologies for differentiating macrophages often lack precision. They are [...] Read more.
Macrophages, known for their phenotypic plasticity, play a critical role in maintaining homeostasis and inflammation-related pathogenesis. Although identifying diverse macrophage phenotypes holds promise for enhancing diagnoses and treatments of diseases mediated by macrophages, existing methodologies for differentiating macrophages often lack precision. They are limited by the cumbersome procedures that require large-scale equipment, such as flow cytometry and transcriptomic analysis. In this context, we have engineered fluorescent polyadenine (polyA)-mediated sticky flares that enable practical visualization of macrophages. This technology facilitates the highly sensitive detection of macrophage phenotypes through the specific recognition of intracellular mRNAs, permitting in situ imaging. Our approach demonstrates the potential for determining macrophage polarization status at the single-cell level within dynamic immune microenvironments, thereby providing crucial diagnostic and prognostic information that could guide the development of tailored treatments for macrophage-related diseases in personalized medicine. Full article
(This article belongs to the Special Issue Optical Bioimaging and Biosensing)
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Review

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19 pages, 6268 KiB  
Review
NIR-II Fluorescent Probes for Fluorescence-Imaging-Guided Tumor Surgery
by Zia Ullah, Shubham Roy, Jingshi Gu, Sai Ko Soe, Jian Jin and Bing Guo
Biosensors 2024, 14(6), 282; https://doi.org/10.3390/bios14060282 - 30 May 2024
Cited by 7 | Viewed by 4183
Abstract
Second near-infrared (NIR-II) fluorescence imaging is the most advanced imaging fidelity method with extraordinary penetration depth, signal-to-background ratio, biocompatibility, and targeting ability. It is currently booming in the medical realm to diagnose tumors and is being widely applied for fluorescence-imaging-guided tumor surgery. To [...] Read more.
Second near-infrared (NIR-II) fluorescence imaging is the most advanced imaging fidelity method with extraordinary penetration depth, signal-to-background ratio, biocompatibility, and targeting ability. It is currently booming in the medical realm to diagnose tumors and is being widely applied for fluorescence-imaging-guided tumor surgery. To efficiently execute this modern imaging modality, scientists have designed various probes capable of showing fluorescence in the NIR-II window. Here, we update the state-of-the-art NIR-II fluorescent probes in the most recent literature, including indocyanine green, NIR-II emissive cyanine dyes, BODIPY probes, aggregation-induced emission fluorophores, conjugated polymers, donor–acceptor–donor dyes, carbon nanotubes, and quantum dots for imaging-guided tumor surgery. Furthermore, we point out that the new materials with fluorescence in NIR-III and higher wavelength range to further optimize the imaging results in the medical realm are a new challenge for the scientific world. In general, we hope this review will serve as a handbook for researchers and students who have an interest in developing and applying fluorescent probes for NIR-II fluorescence-imaging-guided surgery and that it will expedite the clinical translation of the probes from bench to bedside. Full article
(This article belongs to the Special Issue Optical Bioimaging and Biosensing)
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