Special Issue "Stem Cell and Biologic Scaffold Engineering"

A special issue of Bioengineering (ISSN 2306-5354).

Deadline for manuscript submissions: 31 July 2019

Special Issue Editor

Guest Editor
Dr. Panagiotis Mallis

Department of Tissue Engineering and Regenerative Medicine of Hellenic Cord Blood Bank, Biomedical Research Foundation Academy of Athens (BRFAA), Greece
E-Mail
Interests: Tissue Engineered Small Diameter Vascular Grafts; Decellularized human umbilical artery; Mesenchymal Stromal Cells; Hematopoietic Stem Cells; Platelet Rich Plasma

Special Issue Information

Dear Colleagues,

Tissue engineering and regenerative medicine is a rapidly evolving research field, which combines effectively stem cells and biologic scaffolds in order to replace damaged tissues. Biologic scaffolds can be produced through the removal of resident cellular populations using several tissue engineering approaches such as the decellularization method. Indeed, the decellularization method aims to develop a cell-free biologic scaffold, while the extracellular matrix (ECM) could be preserved intact. Furthermore, biologic scaffolds have been investigated for in vitro potential of whole organ development. Currently, clinical products composed of decellularized matrices such as pericardium, urinary bladder, small intestine, heart valves, nerve conduits, trachea, and vessels are being evaluated in order to be used in human clinical trials.

Tissue engineering strategies require the interaction of biologic scaffolds with cellular populations. Among them, stem cells are characterized by unlimited cell division, self-renewal, and differentiation potential, distinguishing themselves as a frontline source for the repopulation of decellularized matrices and scaffolds. Under this scope, stem cells can be isolated from patients, expanded under Good Manufacturing Practices conditions (GMPs), used for the repopulation of biologic scaffolds, and, finally, returned to the patient. The interaction between scaffolds and stem cells is thought to be crucial for their infiltration, adhesion, and differentiation into specific cell types. In addition, biomedical devices such as bioreactors contribute to the uniform repopulation of scaffolds.

Until now, a remarkable effort has been performed by the scientific society in order to establish the proper repopulation conditions of decellularized matrices and scaffolds. However, parameters such as stem cell number, in vitro cultivation conditions, and specific growth media composition need further evaluation. The ultimate goal is the development of “artificial” tissues similar to native ones, which is achieved by combining properly stem cells and biologic scaffolds, thus bringing them one step closer to personalized medicine.

This Special Issue will accept original research articles and comprehensive reviews that deal with the use of stem cells and biologic scaffolds that utilize state-of-the-art tissue engineering and regenerative medicine approaches.

We look forward to receiving your valuable contributions to this Special Issue.

Dr. Panagiotis Mallis
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Bioengineering is an international peer-reviewed open access quarterly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 550 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (5 papers)

View options order results:
result details:
Displaying articles 1-5
Export citation of selected articles as:

Research

Jump to: Other

Open AccessArticle Administration of Adipose Derived Mesenchymal Stem Cells and Platelet Lysate in Erectile Dysfunction: A Single Center Pilot Study
Bioengineering 2019, 6(1), 21; https://doi.org/10.3390/bioengineering6010021
Received: 16 January 2019 / Revised: 26 February 2019 / Accepted: 28 February 2019 / Published: 5 March 2019
PDF Full-text (2140 KB) | HTML Full-text | XML Full-text | Supplementary Files
Abstract
Erectile dysfunction (ED) affects more than 30 million men; endothelial dysfunction plays a significant role in EDs pathogenesis. The aim of this study was to administer mesenchymal stem cells (MSC) derived from adipose tissue and platelet lysate (PL) into patients with erectile dysfunction. [...] Read more.
Erectile dysfunction (ED) affects more than 30 million men; endothelial dysfunction plays a significant role in EDs pathogenesis. The aim of this study was to administer mesenchymal stem cells (MSC) derived from adipose tissue and platelet lysate (PL) into patients with erectile dysfunction. This pilot study enrolled eight patients with diagnosed ED. Patients enrolled were suffering from organic ED due to diabetes melitus, hypertension, hypercholesterolaemia, and Peyronie disease. The patients were distributed in 2 groups. Patients in group A received adipose derived mesenchymal stem cells (ADMSC) resuspended in PL while patients in group B received only PL. ADMSCs were isolated from patients’ adipose tissue and expanded. In addition, blood sampling was obtained from the patients in order to isolate platelet lysate. After the application of the above treatments, patients were evaluated with an International Index of Erectile Function (IIEF-5) questionnaire, penile triplex, and reported morning erections. After MSCs and PL administration, patients presented improved erectile function after 1 and 3 months of follow-up. A statistically significant difference was observed in the IIEF-5 score before and after administration of both treatments after the first month (p < 0.05) and the third month (p < 0.05). No statistically significant difference was observed in the IIEF-5 score between group A and B patients. All patients were characterized by improved penile triplex and increased morning erections. No severe adverse reactions were observed in any patient except a minor pain at the site of injection, which was in the limits of tolerability. The results of this study indicated the satisfactory use of MSCs and PL in ED. MSCs in combination with PL or PL alone seems to be very promising, especially without having the negative effects of the current therapeutic treatment. Full article
(This article belongs to the Special Issue Stem Cell and Biologic Scaffold Engineering)
Figures

Graphical abstract

Open AccessCommunication Optimization of Decellularization Procedure in Rat Esophagus for Possible Development of a Tissue Engineered Construct
Bioengineering 2019, 6(1), 3; https://doi.org/10.3390/bioengineering6010003
Received: 5 December 2018 / Revised: 19 December 2018 / Accepted: 20 December 2018 / Published: 24 December 2018
PDF Full-text (1445 KB) | HTML Full-text | XML Full-text | Supplementary Files
Abstract
Background: Current esophageal treatment is associated with significant morbidity. The gold standard therapeutic strategies are stomach interposition or autografts derived from the jejunum and colon. However, severe adverse reactions, such as esophageal leakage, stenosis and infection, accompany the above treatments, which, most times, [...] Read more.
Background: Current esophageal treatment is associated with significant morbidity. The gold standard therapeutic strategies are stomach interposition or autografts derived from the jejunum and colon. However, severe adverse reactions, such as esophageal leakage, stenosis and infection, accompany the above treatments, which, most times, are life threating. The aim of this study was the optimization of a decellularization protocol in order to develop a proper esophageal tissue engineered construct. Methods: Rat esophagi were obtained from animals and were decellularized. The decellularization process involved the use of 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) and sodium dodecyl sulfate (SDS) buffers for 6 h each, followed by incubation in a serum medium. The whole process involved two decellularization cycles. Then, a histological analysis was performed. In addition, the amounts of collagen, sulphated glycosaminoglycans and DNA content were quantified. Results: The histological analysis revealed that only the first decellularization cycle was enough to produce a cellular and nuclei free esophageal scaffold with a proper extracellular matrix orientation. These results were further confirmed by biochemical quantification. Conclusions: Based on the above results, the current decellularization protocol can be applied successfully in order to produce an esophageal tissue engineered construct. Full article
(This article belongs to the Special Issue Stem Cell and Biologic Scaffold Engineering)
Figures

Graphical abstract

Open AccessArticle Decellularized Human Umbilical Artery Used as Nerve Conduit
Bioengineering 2018, 5(4), 100; https://doi.org/10.3390/bioengineering5040100
Received: 28 September 2018 / Revised: 8 November 2018 / Accepted: 16 November 2018 / Published: 21 November 2018
PDF Full-text (3150 KB) | HTML Full-text | XML Full-text
Abstract
Treatment of injuries to peripheral nerves after a segmental defect is one of the most challenging surgical problems. Despite advancements in microsurgical techniques, complete recovery of nerve function after repair has not been achieved. The purpose of this study was to evaluate the [...] Read more.
Treatment of injuries to peripheral nerves after a segmental defect is one of the most challenging surgical problems. Despite advancements in microsurgical techniques, complete recovery of nerve function after repair has not been achieved. The purpose of this study was to evaluate the use of the decellularized human umbilical artery (hUA) as nerve guidance conduit. A segmental peripheral nerve injury was created in 24 Sprague–Dawley rats. The animals were organized into two experimental groups with different forms of repair: decellularized hUA (n = 12), and autologous nerve graft (n = 12). Sciatic faction index and gastrocnemius muscle values were calculated for functional recovery evaluation. Nerve morphometry was used to analyze nerve regeneration. Results showed that decellularized hUAs after implantation were rich in nerve fibers and characterized by improved Sciatic Functional index (SFI) values. Decellularized hUA may support elongation and bridging of the 10 mm nerve gap. Full article
(This article belongs to the Special Issue Stem Cell and Biologic Scaffold Engineering)
Figures

Figure 1

Open AccessArticle Evaluation of HLA-G Expression in Multipotent Mesenchymal Stromal Cells Derived from Vitrified Wharton’s Jelly Tissue
Bioengineering 2018, 5(4), 95; https://doi.org/10.3390/bioengineering5040095
Received: 18 October 2018 / Revised: 27 October 2018 / Accepted: 31 October 2018 / Published: 1 November 2018
Cited by 1 | PDF Full-text (7771 KB) | HTML Full-text | XML Full-text | Supplementary Files
Abstract
Background: Mesenchymal Stromal Cells (MSCs) from Wharton’s Jelly (WJ) tissue express HLA-G, a molecule which exerts several immunological properties. This study aimed at the evaluation of HLA-G expression in MSCs derived from vitrified WJ tissue. Methods: WJ tissue samples were isolated from human [...] Read more.
Background: Mesenchymal Stromal Cells (MSCs) from Wharton’s Jelly (WJ) tissue express HLA-G, a molecule which exerts several immunological properties. This study aimed at the evaluation of HLA-G expression in MSCs derived from vitrified WJ tissue. Methods: WJ tissue samples were isolated from human umbilical cords, vitrified with the use of VS55 solution and stored for 1 year at −196 °C. After 1 year of storage, the WJ tissue was thawed and MSCs were isolated. Then, MSCs were expanded until reaching passage 8, followed by estimation of cell number, cell doubling time (CDT), population doubling (PD) and cell viability. In addition, multilineage differentiation, Colony-Forming Units (CFUs) assay and immunophenotypic analyses were performed. HLA-G expression in MSCs derived from vitrified samples was evaluated by immunohistochemistry, RT-PCR/PCR, mixed lymphocyte reaction (MLR) and immunofluorescence. MSCs derived from non-vitrified WJ tissue were used in order to validate the results obtained from the above methods. Results: MSCs were successfully obtained from vitrified WJ tissues retaining their morphological and multilineage differentiation properties. Furthermore, MSCs from vitrified WJ tissues successfully expressed HLA-G. Conclusion: The above results indicated the successful expression of HLA-G by MSCs from vitrified WJ tissues, thus making them ideal candidates for immunomodulation. Full article
(This article belongs to the Special Issue Stem Cell and Biologic Scaffold Engineering)
Figures

Graphical abstract

Other

Jump to: Research

Open AccessOpinion Introducing the Language of “Relativity” for New Scaffold Categorization
Bioengineering 2019, 6(1), 20; https://doi.org/10.3390/bioengineering6010020
Received: 10 January 2019 / Revised: 12 February 2019 / Accepted: 22 February 2019 / Published: 26 February 2019
PDF Full-text (202 KB) | HTML Full-text | XML Full-text
Abstract
Research related with scaffold engineering tends to be cross-domain and miscellaneous. Several realms may need to be focused simultaneously, including biomedicine for cell culture and 3D scaffold, physics for dynamics, manufacturing for technologies like 3D printing, chemistry for material composition, as well as [...] Read more.
Research related with scaffold engineering tends to be cross-domain and miscellaneous. Several realms may need to be focused simultaneously, including biomedicine for cell culture and 3D scaffold, physics for dynamics, manufacturing for technologies like 3D printing, chemistry for material composition, as well as architecture for scaffold’s geometric control. As a result, researchers with different backgrounds sometimes could have different understanding towards the product described as ‘Scaffold’. After reviewing the literature, numerous studies termed their developed scaffold as ‘novel’, compared with scaffolds previously designed by others using comparing criterion like ‘research time’, ‘manufacturing method’, ‘geometry’, and so on. While it may have been convenient a decade ago to, for example, categorize scaffold with ‘Dualistic Thinking’ logic into ‘simple-complicated’ or ‘traditional-novel’, this method for categorizing ‘novelty’ and distinguishing scaffold is insufficiently persuasive and precise when it comes to modern or future scaffold. From this departure of philosophical language, namely the language of ‘relativity’, it is important to distinguish between different scaffolds. Other than attempting to avoid ambiguity in perceiving scaffold, this language also provides clarity regarding the ‘evolution stage’ where the focused scaffolds currently stand, where they have been developed, and where in future they could possibly evolve. Full article
(This article belongs to the Special Issue Stem Cell and Biologic Scaffold Engineering)
Bioengineering EISSN 2306-5354 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top