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Bioengineering
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Stem Cell-Based Approaches for Treatment of Lung Disease
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Stem Cell-Based Approaches for Treatment of Lung Disease

A special issue of Bioengineering (ISSN 2306-5354). This special issue belongs to the section "Regenerative Engineering".

Deadline for manuscript submissions: closed (20 March 2023) | Viewed by 2470

Special Issue Editor

Dr. Golnaz Karoubi

E-Mail Website
Guest Editor
Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Canada
Interests: lung; airways; trachea; regenerative medicine; stem cells; tissue engineering; bioengineering; bioreactors; biomimetic culture; aging; pulmonary disease; cystic fibrosis; pulmonary fibrosis

Special Issue Information

Dear Colleagues,

Airway disease, both acute and chronic, remains a major healthcare burden and is expected to increase with an aging population. Altering the course of these intractable diseases will require innovative and novel approaches to understand the underlying mechanisms of disease and develop therapeutic alternatives with a realistic path to clinical translation.

This Special Issue of Bioengineering on Stem Cell Approaches for Treatment of Lung and Airway Disease will highlight the application of stem cells in the lung, bringing together contributions from leading experts across the globe. Content will encompass original research and comprehensive reviews focused on the application of stem cells for (1) cell-based therapeutic approaches for attenuation and/or abrogation of disease, (2) ex vivo cell culture systems as platforms for disease modeling and therapeutics, and (3) tissue regeneration for replacement of damaged lung and airway tissue.

Dr. Golnaz Karoubi
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Bioengineering is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • lung disease
  • stem cells
  • cell therapy
  • lung bioengineering

Published Papers (2 papers)

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Research

14 pages, 4438 KiB  
Article
Orthotopic Ferret Tracheal Transplantation Using a Recellularized Bioengineered Graft Produces Functional Epithelia
by Albert C. Pai, Anthony M. Swatek, Thomas J. Lynch, Bethany A. Ahlers, Vitaly Ievlev, John F. Engelhardt and Kalpaj R. Parekh
Bioengineering 2023, 10(7), 777; https://doi.org/10.3390/bioengineering10070777 - 29 Jun 2023
Viewed by 945
Abstract
Tracheal grafts may be necessary to bridge long-segment defects after curative resection for airway obstructions. Bioengineered grafts have emerged as an appealing option, given the possibilities of altering the histologic and cellular profile of the conduit. We previously designed a bioreactor capable of [...] Read more.
Tracheal grafts may be necessary to bridge long-segment defects after curative resection for airway obstructions. Bioengineered grafts have emerged as an appealing option, given the possibilities of altering the histologic and cellular profile of the conduit. We previously designed a bioreactor capable of luminally decellularizing and recellularizing a ferret trachea with surface airway epithelia (SAE) basal cells (BCs), and we sought to assess the fate of these grafts when transplanted in an orthotopic fashion. As adjuncts to the procedure, we investigated the use of a vascular endothelial growth factor (VEGF)-laden hydrogel and of immunosuppression (IS) in graft revascularization and viability. IS was shown to limit early graft revascularization, but this effect could be counteracted with VEGF supplementation. Submucosal gland (SMG) loss was shown to be inevitable regardless of the revascularization strategy. Lastly, the bioengineered tracheas survived one month after transplant with differentiation of our implanted BCs that then transitioned into a recipient-derived functional epithelium. The work presented in this manuscript has important implications for future cellular and regenerative therapies. Full article
(This article belongs to the Special Issue Stem Cell-Based Approaches for Treatment of Lung Disease)
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13 pages, 2083 KiB  
Article
Evaluation of Structural Viability of Porcine Tracheal Scaffolds after 3 and 6 Months of Storage under Three Different Protocols
by Alberto Bruning Guimaraes, Aristides Tadeu Correia, Ronaldo Soares da Silva, Elizabete Silva dos Santos, Natalia de Souza Xavier Costa, Marisa Dolhnikoff, Marina Maizato, Idagene Aparecida Cestari, Paulo Manuel Pego-Fernandes and Paulo Francisco Guerreiro Cardoso
Bioengineering 2023, 10(5), 584; https://doi.org/10.3390/bioengineering10050584 - 12 May 2023
Cited by 1 | Viewed by 1137
Abstract
Tracheal replacement with a bioengineered tracheal substitute has been developed for long-segment tracheal diseases. The decellularized tracheal scaffold is an alternative for cell seeding. It is not defined if the storage scaffold produces changes in the scaffold’s biomechanical properties. We tested three protocols [...] Read more.
Tracheal replacement with a bioengineered tracheal substitute has been developed for long-segment tracheal diseases. The decellularized tracheal scaffold is an alternative for cell seeding. It is not defined if the storage scaffold produces changes in the scaffold’s biomechanical properties. We tested three protocols for porcine tracheal scaffold preservation immersed in PBS and alcohol 70%, in the fridge and under cryopreservation. Ninety-six porcine tracheas (12 in natura, 84 decellularized) were divided into three groups (PBS, alcohol, and cryopreservation). Twelve tracheas were analyzed after three and six months. The assessment included residual DNA, cytotoxicity, collagen contents, and mechanical properties. Decellularization increased the maximum load and stress in the longitudinal axis and decreased the maximum load in the transverse axis. The decellularization of the porcine trachea produced structurally viable scaffolds, with a preserved collagen matrix suitable for further bioengineering. Despite the cyclic washings, the scaffolds remained cytotoxic. The comparison of the storage protocols (PBS at 4 °C, alcohol at 4 °C, and slow cooling cryopreservation with cryoprotectants) showed no significant differences in the amount of collagen and in the biomechanical properties of the scaffolds. Storage in PBS solution at 4 °C for six months did not change the scaffold mechanics. Full article
(This article belongs to the Special Issue Stem Cell-Based Approaches for Treatment of Lung Disease)
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