Organoid Cultures: Revolutionizing Tissue Engineering and Therapeutics for Precision Medicine

A special issue of Bioengineering (ISSN 2306-5354). This special issue belongs to the section "Regenerative Engineering".

Deadline for manuscript submissions: closed (31 August 2024) | Viewed by 3172

Special Issue Editor


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Guest Editor
Department of Neurology and Neurosurgery, Division of Brain Tumor, School of Medicine, The Johns Hopkins University, Baltimore, MD 21218, USA
Interests: biomaterials; tissue engineering; regenerative medicine; precision medicine; organoid cultures

Special Issue Information

Dear Colleagues,

This Special Issue of Bioengineering, titled "Organoid Cultures: Revolutionizing Tissue Engineering and Therapeutics for Precision Medicine," is dedicated to exploring the profound impact of organoid cultures on advancing the fields of tissue engineering and therapeutics, with a specific focus on their role in shaping the landscape of precision medicine.

Organoids, three-dimensional structures that replicate the architecture and function of native organs, have emerged as transformative tools in biomedical research. We invite researchers and experts from diverse disciplines to contribute original research and review articles that shed light on recent advances in the use of organoid cultures.

The featured articles aim to provide a comprehensive overview of the state-of-the-art methodologies and applications of organoid cultures in precision medicine. Researchers are encouraged to delve into topics such as engineering strategies for organoid development, innovative biomaterials, and the therapeutic implications of organoid-based approaches. This Special Issue seeks to capture the multidisciplinary nature of this rapidly evolving field, showcasing contributions that span bioengineering, cell biology, regenerative medicine, and clinical applications.

Organoids hold tremendous promise for precision medicine due to their ability to faithfully recapitulate the complexity of human tissues. They serve as invaluable models for studying disease mechanisms, drug responses, and patient-specific treatment strategies. Through this Special Issue, we aim to foster a deeper understanding of the potential and challenges of using organoid cultures, creating a dialogue to accelerate the translation of research findings into clinical applications.

As we embark on this journey through the realms of organoid-based tissue engineering and therapeutics, we anticipate that the collective knowledge shared within this Special Issue will catalyze further innovations, inspire collaborative efforts, and contribute to the ongoing evolution of precision medicine. We invite you to engage with the diverse perspectives presented here and join us in shaping the future of organoid research for the benefit of personalized healthcare.

Dr. Sanika Suvarnapathaki
Guest Editor

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Keywords

  • organoid cultures
  • tissue engineering
  • precision medicine
  • therapeutic biomaterials
  • regenerative medicine

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Published Papers (1 paper)

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Research

11 pages, 4821 KiB  
Article
Formation and Long-Term Culture of hiPSC-Derived Sensory Nerve Organoids Using Microfluidic Devices
by Takuma Ogawa, Souichi Yamada, Shuetsu Fukushi, Yuya Imai, Jiro Kawada, Kazutaka Ikeda, Seii Ohka and Shohei Kaneda
Bioengineering 2024, 11(8), 794; https://doi.org/10.3390/bioengineering11080794 - 5 Aug 2024
Viewed by 2874
Abstract
Although methods for generating human induced pluripotent stem cell (hiPSC)-derived motor nerve organoids are well established, those for sensory nerve organoids are not. Therefore, this study investigated the feasibility of generating sensory nerve organoids composed of hiPSC-derived sensory neurons using a microfluidic approach. [...] Read more.
Although methods for generating human induced pluripotent stem cell (hiPSC)-derived motor nerve organoids are well established, those for sensory nerve organoids are not. Therefore, this study investigated the feasibility of generating sensory nerve organoids composed of hiPSC-derived sensory neurons using a microfluidic approach. Notably, sensory neuronal axons from neurospheres containing 100,000 cells were unidirectionally elongated to form sensory nerve organoids over 6 mm long axon bundles within 14 days using I-shaped microchannels in microfluidic devices composed of polydimethylsiloxane (PDMS) chips and glass substrates. Additionally, the organoids were successfully cultured for more than 60 days by exchanging the culture medium. The percentage of nuclei located in the distal part of the axon bundles (the region 3−6 mm from the entrance of the microchannel) compared to the total number of cells in the neurosphere was 0.005% for live cells and 0.008% for dead cells. Molecular characterization confirmed the presence of the sensory neuron marker ISL LIM homeobox 1 (ISL1) and the capsaicin receptor transient receptor potential vanilloid 1 (TRPV1). Moreover, capsaicin stimulation activated TRPV1 in organoids, as evidenced by significant calcium ion influx. Conclusively, this study demonstrated the feasibility of long-term organoid culture and the potential applications of sensory nerve organoids in bioengineered nociceptive sensors. Full article
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