New Sights of 3D Printing in Bioengineering: Updates and Directions

A special issue of Bioengineering (ISSN 2306-5354). This special issue belongs to the section "Nanobiotechnology and Biofabrication".

Deadline for manuscript submissions: closed (30 June 2024) | Viewed by 5484

Special Issue Editors


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Guest Editor
Division of Molecular Pharmaceutics and Drug Delivery, College of Pharmacy, University of Texas at Austin, Austin, TX 78712, USA
Interests: 3D bioprinting; 3D printing; continuous manufacturing; drug formulation and delivery; implantable medical devices; smart biomaterials
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Guest Editor
Department of Pharmaceutical Sciences and Experimental Therapeutics, The University of Iowa, Iowa City, IA, USA
Interests: 3D printing; devices; gas sensing; nanoparticles

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Guest Editor
Division of Molecular Pharmaceutics and Drug Delivery, College of Pharmacy, University of Texas at Austin, Austin, TX 78712, USA
Interests: 3D printing; devices; nanoparticles

Special Issue Information

Dear Colleagues,

A few years ago, researchers have been inspired by the promise of 3D Printing to produce complex biomedical devices, design better instruments, create patient specific devices, deliver drugs, replace lost tissues, guide wound healing, and regenerate tissues. From pre-surgical models to tooling molds and instrument prototypes, 3D Printing has evolved to create one-of-a-kind devices, implants, scaffolds for tissue engineering, diagnostic platforms, and drug delivery systemsThis highlights the fact that, numerous technical, regulatory, and commercial limitations must be overcome before 3D Printing can reach its full potential in biomedical applications.

The aim of this Special Issue, entitled “New sight of 3D printing in bioengineering: Updates and Directions, is to make relevant work known to our colleagues in the field. To achieve this, the Special Issue, edited by Professor Mo Maniruzzaman and Dr. L. R. Jaidev,  Dr. Niloofar Heshmati Aghda, invites scientists to submit research articles, review articles, and short communications focused on this topic.

We look forward to your valuable contributions to make this Special Issue a reference resource for future researchers in the field of 3D printing in bioengineering.

Dr. Mo Maniruzzaman
Dr. L. R. Jaidev
Dr. Niloofar Heshmati Aghda
Guest Editors

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Published Papers (2 papers)

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Research

16 pages, 4735 KiB  
Article
Composites of Polylactic Acid with Diatomaceous Earth for 3D-Printing Biocompatible Scaffolds: A Systematic Study of Their Mechanical, Thermal, and Biocompatibility Properties
by Lilliam Trejos-Soto, Gabriel O. Rivas-Hernández, Rodrigo Mora-Bolaños, Nathalia Vargas-Valverde, Abraham Valerio, Andrea Ulloa-Fernández, Jorge Oviedo-Quirós, Alfonso García-Piñeres, Sergio A. Paniagua, Carolina Centeno-Cerdas and Leonardo Lesser-Rojas
Bioengineering 2024, 11(11), 1059; https://doi.org/10.3390/bioengineering11111059 - 24 Oct 2024
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Abstract
This study explores the development of biocompatible scaffolds for bone regeneration, utilizing polylactic acid (PLA) combined with calcium phosphate as a pH buffer and diatomaceous earth as a biocompatibilizer. These materials were extruded and 3D-printed to enhance cell adhesion and biodegradability after enough [...] Read more.
This study explores the development of biocompatible scaffolds for bone regeneration, utilizing polylactic acid (PLA) combined with calcium phosphate as a pH buffer and diatomaceous earth as a biocompatibilizer. These materials were extruded and 3D-printed to enhance cell adhesion and biodegradability after enough cell growth. The biocompatibility of the resulting composites, with different proportions of the components and sterilization methods, was tested according to the ISO 10993 protocol. The optimal performance, with nearly zero cytotoxicity, was observed with 20 PLA/1 CP/1 DE mass ratios and gamma sterilization. Tension analysis and scanning electron microscopy (SEM) were applied to the 3D-printed composites, which were also analyzed by differential scanning calorimetry (DSC) to understand the origin of the tension properties better, which were comparable to those of cancellous bone. Degradation tests under physiological conditions for 13 weeks showed no significant mass loss. Furthermore, it was observed that cell adhesion, viability, proliferation, and osteoconduction are possible in the scaffolds studied, opening opportunities for future studies to substantiate the use of 3D-printed silica-filled composites as an alternative to homologous implants for various bone regeneration applications. Full article
(This article belongs to the Special Issue New Sights of 3D Printing in Bioengineering: Updates and Directions)
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16 pages, 12602 KiB  
Article
Advancing 3D Spheroid Research through 3D Scaffolds Made by Two-Photon Polymerization
by Eglė Vitkūnaitė, Eglė Žymantaitė, Agata Mlynska, Dovilė Andrijec, Karolina Limanovskaja, Grzegorz Kaszynski, Daumantas Matulis, Vidmantas Šakalys and Linas Jonušauskas
Bioengineering 2024, 11(9), 902; https://doi.org/10.3390/bioengineering11090902 - 9 Sep 2024
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Abstract
Three-dimensional cancer cell cultures have been a valuable research model for developing new drug targets in the preclinical stage. However, there are still limitations to these in vitro models. Scaffold-based systems offer a promising approach to overcoming these challenges in cancer research. In [...] Read more.
Three-dimensional cancer cell cultures have been a valuable research model for developing new drug targets in the preclinical stage. However, there are still limitations to these in vitro models. Scaffold-based systems offer a promising approach to overcoming these challenges in cancer research. In this study, we show that two-photon polymerization (TPP)-assisted printing of scaffolds enhances 3D tumor cell culture formation without additional modifications. TPP is a perfect fit for this task, as it is an advanced 3D-printing technique combining a μm-level resolution with complete freedom in the design of the final structure. Additionally, it can use a wide array of materials, including biocompatible ones. We exploit these capabilities to fabricate scaffolds from two different biocompatible materials—PEGDA and OrmoClear. Cubic spheroid scaffolds with a more complex architecture were produced and tested. The biological evaluation showed that the human ovarian cancer cell lines SKOV3 and A2780 formed 3D cultures on printed scaffolds without a preference for the material. The gene expression evaluation showed that the A2780 cell line exhibited substantial changes in CDH1, CDH2, TWIST, COL1A1, and SMAD3 gene expression, while the SKOV3 cell line had slight changes in said gene expression. Our findings show how the scaffold architecture design impacts tumor cell culture 3D spheroid formation, especially for the A2780 cancer cell line. Full article
(This article belongs to the Special Issue New Sights of 3D Printing in Bioengineering: Updates and Directions)
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