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Methods and Protocols, Volume 2, Issue 4

December 2019 - 13 articles

Cover Story: Hypertrophic cardiomyopathy is a prevalent and complex cardiovascular disease with no effective therapeutics available. Disease modeling approaches have often generated contradictory results due to various model limitations and/or methodological constraints. Here, we provide three technically-refined and unbiased high-throughput protocols to phenotype cellular models of this disease. These will facilitate the discovery of novel pathological molecular mechanisms towards more efficient drug screening strategies to treat this condition. View this paper.
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Articles (13)

  • Article
  • Open Access
4 Citations
3,095 Views
21 Pages

11 December 2019

The DosiVox programme is used to reconstruct radiation transport in complex depositional environments. Using two archaeological case studies from ancient Egypt, the burial environments for a selection of ceramic vessels are reconstructed using the Do...

  • Article
  • Open Access
4 Citations
4,028 Views
12 Pages

Testing Luminescence Dating Methods for Small Samples from Very Young Fluvial Deposits

  • Joel Q. G. Spencer,
  • Sébastien Huot,
  • Allen W. Archer and
  • Marcellus M. Caldas

6 December 2019

The impetus behind this study is to understand the sedimentological dynamics of very young fluvial systems in the Amazon River catchment and relate these to land use change and modern analogue studies of tidal rhythmites in the geologic record. Initi...

  • Protocol
  • Open Access
9 Citations
8,315 Views
7 Pages

Optimized Extraction of Insect Genomic DNA for Long-Read Sequencing

  • Brenda Oppert,
  • Samantha Stoss,
  • Alaysha Monk and
  • Timothy Smith

23 November 2019

Long-read sequencing technologies continue to increase the length of reads, and at present can average read lengths of >20 kb up to 60–80 kb. Now the challenge is to extract genomic DNA of sufficient fragment size and quality to support long...

  • Article
  • Open Access
14 Citations
5,934 Views
15 Pages

Sampling Methods for Luminescence Dating of Subsurface Deposits from Cores

  • Michelle Nelson,
  • Tammy Rittenour and
  • Harriet Cornachione

22 November 2019

Study of subsurface deposits often requires coring or drilling to obtain samples for sedimentologic and geochemical analysis. Geochronology is a critical piece of information for stratigraphic correlation and rate calculations. Increasingly, luminesc...

  • Protocol
  • Open Access
1 Citations
3,426 Views
7 Pages

21 November 2019

The grain transfer protocol presents a step-by-step guide on how to successfully transfer positioned grains from a single-grain luminescence disc to a scanning electron microscope (SEM) specimen stub and how to transport them between laboratories. Si...

  • Protocol
  • Open Access
29 Citations
13,282 Views
15 Pages

A Method to Reuse Archived H&E Stained Histology Slides for a Multiplex Protein Biomarker Analysis

  • James P. Hinton,
  • Katerina Dvorak,
  • Esteban Roberts,
  • Wendy J. French,
  • Jon C. Grubbs,
  • Anne E. Cress,
  • Hina A. Tiwari and
  • Raymond B. Nagle

15 November 2019

Archived Hematoxylin and Eosin (H&E) stained pathology slides are routinely stored to index formalin-fixed paraffin-embedded (FFPE) sample tissue blocks. FFPE blocks are clinically annotated human tumor specimens that can be valuable in studies d...

  • Protocol
  • Open Access
7 Citations
4,823 Views
41 Pages

Characterising Mutational Spectra of Carcinogens in the Tumour Suppressor Gene TP53 Using Human TP53 Knock-in (Hupki) Mouse Embryo Fibroblasts

  • Lisa Hölzl-Armstrong,
  • Jill E. Kucab,
  • Michael Korenjak,
  • Mirjam Luijten,
  • David H. Phillips,
  • Jiri Zavadil and
  • Volker M. Arlt

13 November 2019

DNA in dividing cells is prone to mutagenesis, with mutations making key contributions to human disease including cancer. The tumour suppressor gene TP53 is the most frequently mutated gene in human tumours. Here, we present a robust protocol for stu...

  • Feature Paper
  • Article
  • Open Access
4 Citations
3,377 Views
11 Pages

Alkaline Extraction for Lead Determination in Different Types of Commercial Paints

  • David Romero-Estévez,
  • Gabriela S. Yánez-Jácome,
  • Karina Simbaña-Farinango,
  • Pamela Y Vélez-Terreros and
  • Hugo Navarrete

1 November 2019

In 2017, the World Health Organization and the United Nations Environment Programme formed the Global Alliance to Eliminate Lead Paint. All alliance member countries have pledged to develop control regulations that include lead threshold limits. To i...

  • Protocol
  • Open Access
9 Citations
5,561 Views
26 Pages

26 October 2019

Hypertrophic cardiomyopathy (HCM) is a prevalent and complex cardiovascular disease characterised by multifarious hallmarks, a heterogeneous set of clinical manifestations, and several molecular mechanisms. Various disease models have been developed...

  • Article
  • Open Access
16 Citations
3,001 Views
13 Pages

Assessing Pancreas Transplant Candidate Cardiac Disease: Preoperative Protocol Development at a Rapidly Growing Transplant Program

  • David St. Michel,
  • Tracy Donnelly,
  • Towanda Jackson,
  • Bradley Taylor,
  • Rolf N. Barth,
  • Jonathan S. Bromberg and
  • Joseph R. Scalea

17 October 2019

Pancreas transplant rates, despite improving outcomes, have decreased over the past two decades. This is due, in part, to ageing, increasingly co-morbid pancreas transplant candidates. There is a paucity of published data regarding coronary artery di...

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Methods Protoc. - ISSN 2409-9279