Research Progress on the Effect of Autolysis to Bacillus subtilis Fermentation Bioprocess

Ren, Kexin; Wang, Qiang; Hu, Mengkai; Chen, Yan; Xing, Rufan; You, Jiajia; Xu, Meijuan; Zhang, Xian; Rao, Zhiming

doi:10.3390/fermentation8120685

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Research Progress on the Effect of Autolysis to Bacillus subtilis Fermentation Bioprocess

by

Kexin Ren

^†,

Qiang Wang

^†,

Mengkai Hu

,

Yan Chen

,

Rufan Xing

,

Jiajia You

,

Meijuan Xu

,

Xian Zhang

^*

and

Zhiming Rao

^*

Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214000, China

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Fermentation 2022, 8(12), 685; https://doi.org/10.3390/fermentation8120685

Submission received: 2 November 2022 / Revised: 22 November 2022 / Accepted: 25 November 2022 / Published: 28 November 2022

(This article belongs to the Special Issue Applied Microorganisms and Industrial/Food Enzymes)

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Abstract

Bacillus subtilis is a gram-positive bacterium, a promising microorganism due to its strong extracellular protein secretion ability, non-toxic, and relatively mature industrial fermentation technology. However, cell autolysis during fermentation restricts the industrial application of B. subtilis. With the fast advancement of molecular biology and genetic engineering technology, various advanced procedures and gene editing tools have been used to successfully construct autolysis-resistant B. subtilis chassis cells to manufacture various biological products. This paper first analyses the causes of autolysis in B. subtilis from a mechanistic perspective and outlines various strategies to address autolysis in B. subtilis. Finally, potential strategies for solving the autolysis problem of B. subtilis are foreseen.

Keywords: Bacillus subtilis; cell autolysis; gene editing; fermentation technology; chassis cell

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MDPI and ACS Style

Ren, K.; Wang, Q.; Hu, M.; Chen, Y.; Xing, R.; You, J.; Xu, M.; Zhang, X.; Rao, Z. Research Progress on the Effect of Autolysis to Bacillus subtilis Fermentation Bioprocess. Fermentation 2022, 8, 685. https://doi.org/10.3390/fermentation8120685

AMA Style

Ren K, Wang Q, Hu M, Chen Y, Xing R, You J, Xu M, Zhang X, Rao Z. Research Progress on the Effect of Autolysis to Bacillus subtilis Fermentation Bioprocess. Fermentation. 2022; 8(12):685. https://doi.org/10.3390/fermentation8120685

Chicago/Turabian Style

Ren, Kexin, Qiang Wang, Mengkai Hu, Yan Chen, Rufan Xing, Jiajia You, Meijuan Xu, Xian Zhang, and Zhiming Rao. 2022. "Research Progress on the Effect of Autolysis to Bacillus subtilis Fermentation Bioprocess" Fermentation 8, no. 12: 685. https://doi.org/10.3390/fermentation8120685

APA Style

Ren, K., Wang, Q., Hu, M., Chen, Y., Xing, R., You, J., Xu, M., Zhang, X., & Rao, Z. (2022). Research Progress on the Effect of Autolysis to Bacillus subtilis Fermentation Bioprocess. Fermentation, 8(12), 685. https://doi.org/10.3390/fermentation8120685

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Research Progress on the Effect of Autolysis to Bacillus subtilis Fermentation Bioprocess

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