Abstract
Lactiplantibacillus species are widely recognized for their probiotic potential, yet the genetic determinants underlying strain-specific functional traits remain incompletely characterized. This study aimed to obtain the draft genome sequence of Lactiplantibacillus plantarum CIDCA 83114, a kefir-derived strain with previously demonstrated probiotic and antimicrobial properties, and to identify genetic features potentially associated with these traits and with strain safety. Whole-genome sequencing was performed on an Illumina MiSeq platform, genome assembly was generated using SPAdes, and functional annotation was conducted using Prokka and KEGG-based tools. The genome comprises a 3.27 Mb chromosome and five putative plasmid sequences. KEGG analysis identified 36 complete metabolic pathways, including biosynthetic routes for amino acid and vitamin biosynthesis. A plantaricin gene cluster was identified, consistent with the genetic potential for bacteriocin production. Core-genome phylogenetic analysis positioned Lpb. plantarum CIDCA 83114 among dairy-associated Lpb. plantarum isolates. Importantly, genomic screening revealed no acquired antibiotic-resistance or virulence-associated genes. The few resistance-related loci detected were annotated as resistance-associated but could not be confirmed as acquired resistance determinants; they were chromosomally located and showed no association with the plasmid sequences or prophage-associated regions identified in this assembly. Overall, the genomic features identified in Lpb. plantarum CIDCA 83114 provide a molecular basis consistent with its previously characterized metabolic, probiotic, and antimicrobial properties, while supporting its genomic safety profile.