Journal of Fungi, Volume 9, Issue 9
2023 September - 89 articles
Cover Story: The fungus Pneumocystis jirovecii is responsible for life-threatening pneumonia in humans. To understand the pathogenicity of this fungus, a stable culture system is required. In a meticulous and systematic scientific investigation, we undertook a comprehensive literature review encompassing all animal-derived Pneumocystis species. Based on this review, we optimized the culture methodologies, the medium and growth conditions. Finally, we were able to cultivate P. jirovecii successfully. Our axenic flask culture without feeder cells using our newly developed DMEM-O3 medium was successful for up to 70 days. This long culture was necessary due to the extremely long P. jirovecii generation time of 7 to 14 days. View this paper - Issues are regarded as officially published after their release is announced to the table of contents alert mailing list .
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