Toxics

25 pages, 4055 KB

Open AccessArticle

Effects of Long-Term Heavy Metal Pollution on Microbial Community Structure in Soil

by Qiannuo Mi, Yan Wu, Huaisen Cai, Zuben Xu, Yue Zhao, Ronghao Guan, Xin Fan and Jianhua Guo

Toxics 2025, 13(9), 806; https://doi.org/10.3390/toxics13090806 - 22 Sep 2025

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Abstract

Heavy metal (HM) contamination of soil is a worldwide problem with adverse consequences for the environment and human health. Microorganisms, as the most active fraction in soil, play a pivotal role in assessing changes in soil quality and maintaining ecological equilibrium. Accordingly, screening [...] Read more.

Heavy metal (HM) contamination of soil is a worldwide problem with adverse consequences for the environment and human health. Microorganisms, as the most active fraction in soil, play a pivotal role in assessing changes in soil quality and maintaining ecological equilibrium. Accordingly, screening efficient microorganisms for remediating contaminated soils has emerged as a key research focus. This study employed high-throughput sequencing and conducted in situ field surveys to investigate the impacts of long-term HM pollution with varying severity on soil physicochemical properties, as well as the community structure and diversity of bacteria and fungi. The results showed that the major soil physiochemical properties and the bacterial and fungal β diversity significantly changed with the increase in HM pollution levels. The relative abundances of Chloroflexi, Myxococcota and Nitrospirota among bacteria, along with Chytridiomycota and Talaromyces among fungi, increased significantly with rising HM pollution levels. In low-, medium- and highly contaminated soils, the dominant bacterial species were OTU10618 (Micrococcales), OTU6447 (Chthoniobacterales), and OTU7447 (Burkholderiales), while the dominant fungal species were OTU3669 (Glomerellales), OTU397 (Olpidiales), and OTU2568 (Mortierellales). Bacterial communities were mainly affected by soil-available phosphorus, available cadmium (Cd) and available Pb, while fungal communities were predominantly influenced by soil-available phosphorus, soil organic carbon and total Pb content. These findings demonstrate that soil microorganisms in chronically HM-contaminated soils exhibit adaptive shifts, and this study thereby provides critical implications for assessing the remediation potential of diverse microbial taxa in HM-polluted soils. Full article

(This article belongs to the Special Issue Assessment and Remediation of Heavy Metal Contamination in Soil)

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25 pages, 4979 KB

Open AccessArticle

Single Super Phosphate Improves Lolium perenne Quality and Rhizosphere Microorganism Structure Under Combined Cadmium and Arsenic Stress

by Toe Toe Maw, Jiangdi Deng, Bo Li, Yanqun Zu and Zuran Li

Toxics 2025, 13(9), 805; https://doi.org/10.3390/toxics13090805 - 22 Sep 2025

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Abstract

Cadmium and arsenic co-contamination found in mining actions indicates major effluence in adjacent farmland soils, disturbing the plant physiology and soil’s microbial community. Phosphorus (P) plays a vital role in reducing soil contamination from Cd and As bioavailability and uptake by plants. However, [...] Read more.

Cadmium and arsenic co-contamination found in mining actions indicates major effluence in adjacent farmland soils, disturbing the plant physiology and soil’s microbial community. Phosphorus (P) plays a vital role in reducing soil contamination from Cd and As bioavailability and uptake by plants. However, the right P sources for remediation approaches are critical and still require further research in Cd- and As-contaminated soil. This study aimed to explore the effects of different phosphorus fertilizer sources on Lolium perenne growth and its physiological and rhizosphere microbial diversity under combined contamination with Cd and As. Pot experiments were performed with seven treatments including SSP (single super phosphate), DAP (diammonium phosphate), MAP (monoammonium phosphate), CaP (calcium phosphate), HighCaP (high calcium phosphate), RP (rock phosphate), and no phosphorus fertilizer application (CK) with five replications in the RCB design. The SSP treatment showed the greatest plant height (15.7 cm), hay yield (3567.6 kg·ha⁻¹), and enhanced antioxidant defense activities. It also achieved the highest phosphorus accumulation rate (0.63 g·kg⁻¹) with reduced Cd and As uptake. In addition, SSP promoted higher non-protein sulfhydryl (NPT) and phytochelatin synthetase (PCs) contents along with γ-glutamylcysteine synthetase (γ-ECS) activity, and enriched the rhizosphere microbial community, where the Sphingomonas abundance was 7.08% higher than for other treatments. Therefore, this result indicates that SSP can improve the yield and physiology in L. perenne, as well as soil the rhizosphere microbial community structure, while reducing Cd and As accumulation in plants under Cd and As stress. Full article

(This article belongs to the Special Issue Plant Responses to Heavy Metal)

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16 pages, 5553 KB

Open AccessArticle

Characterization and Source Analysis of Water-Soluble Ions in PM_2.5 at Hainan: Temporal Variation and Long-Range Transport

by Xinghong Xu, Wenshuai Xu, Xinxin Meng, Xiaocong Cao, Biwu Chu, Chuandong Du, Rongfu Xie, Zhaohe Zeng, Hui Sheng, Youjing Lin, Weijun Yan and Hong He

Toxics 2025, 13(9), 804; https://doi.org/10.3390/toxics13090804 - 22 Sep 2025

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Abstract

We explored the mass concentrations of water-soluble ions in PM_2.5 and their variations across different time scales and concentration levels. Using the Positive Matrix Factorization (PMF) model and backward trajectory analysis, we focused on identifying the sources of PM_2.5 and its [...] Read more.

We explored the mass concentrations of water-soluble ions in PM_2.5 and their variations across different time scales and concentration levels. Using the Positive Matrix Factorization (PMF) model and backward trajectory analysis, we focused on identifying the sources of PM_2.5 and its water-soluble ion fractions, with particular emphasis on regional transport. The findings reveal that the average mass concentration of total water-soluble ions in Hainan between 1 August 2021 and 31 July 2022 was 7.0 ± 4.4 µg m⁻³, constituting 73.5% ± 24.4% of PM_2.5. Secondary ions (SO₄²⁻, NO₃⁻, NH₄⁺) were dominant, accounting for 84.0% ± 12.4% of the total water-soluble ions, followed by sea-salt particles. Seasonal variations were pronounced, with the highest concentrations observed in winter and the lowest in summer. The results of the PMF analysis showed that secondary sources, combustion sources, dust sources, and oceanic sources are the main sources of PM_2.5 at the monitoring site. The potential sources and transport pathways of water-soluble ions exhibit distinct seasonal characteristics, with the land-based outflows from the YRD–PRD–Fujian corridor controlling Hainan’s PM_2.5 maxima, while southerly marine air delivers the annual minimum; seasonal alternation between dust/secondary aerosols (winter–spring), combustion (autumn), and oceanic dilution (summer) dictates the island’s air-quality rhythm. Full article

(This article belongs to the Special Issue Source and Components Analysis of Aerosols in Air Pollution)

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41 pages, 2278 KB

Open AccessReview

Heavy Metals and Microplastics as Emerging Contaminants in Bangladesh’s River Systems: Evidence from Urban–Industrial Corridors

by Raju Kumar Das, Mongsathowai Marma, Al Mizan, Gang Chen and Md Shahin Alam

Toxics 2025, 13(9), 803; https://doi.org/10.3390/toxics13090803 - 22 Sep 2025

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Abstract

Urban industrialization is a major driver of water pollution, particularly through emerging contaminants that pose significant health risks for humans and ecosystems. This critical review focuses on Bangladesh’s Buriganga and Dhaleshwari rivers, which pass through highly industrialized and urban areas, analyzing contaminant types, [...] Read more.

Urban industrialization is a major driver of water pollution, particularly through emerging contaminants that pose significant health risks for humans and ecosystems. This critical review focuses on Bangladesh’s Buriganga and Dhaleshwari rivers, which pass through highly industrialized and urban areas, analyzing contaminant types, sources, pathways, and impacts. By synthesizing data from studies published between 2005 and 2024, the paper examines pollutants such as heavy metals (e.g., Cr, Cd, Pb, Ni, Zn, Hg, As, Mn, Cu, Fe) and microplastics in water, sediments, and biota. The Buriganga River shows extreme heavy metal contamination, with surface water Cr concentrations reaching up to 167,160 μg/L, Pb up to 3830 μg/L, and Fe up to 30,000 μg/L, and sediment Cr up to 4249 μg/g, Pb up to 3312 μg/g, and Fe up to 15,435 μg/g. In contrast, the Dhaleshwari River exhibits elevated but comparatively lower heavy metal concentrations in surface water (e.g., Cr up to 3350 μg/L; Cd up to 1890 μg/L; Pb up to 1320 μg/L; Ni up to 1732 μg/L; Fe up to 6040 μg/L) and sediments (Cr up to 282 μg/g; Fe up to 14,375 μg/g). Microplastic contamination in Buriganga is widespread across water, sediments, and biota and dominated by polymers such as polyethylene (PE), polypropylene (PP), and polyethylene terephthalate (PET). Industrial discharges, particularly from the textile, leather, and metal processing industries, are identified as primary sources for heavy metals and microplastics. Additional inputs from domestic waste, agricultural runoff, and municipal sewage intensify pollution, with Cr, Cd, and Pb notably frequently exceeding safety thresholds. Microplastics, originating from municipal waste and atmospheric deposition, persist in these rivers, posing ecological and public health risks. The persistence and bioaccumulation of heavy metals and microplastics threaten aquatic biodiversity by disrupting food chains and pose significant risks to local communities that depend on these rivers for agriculture, fishing, and daily water use. This review highlights the urgent need for comprehensive bioaccumulation studies, long-term monitoring, and enhanced detection techniques to better assess contamination levels. Strengthening environmental regulations, improving waste management, and adopting sustainable industrial practices are critical to mitigating emerging contaminant impacts and safeguarding these vital river ecosystems and public health. Full article

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22 pages, 4983 KB

Open AccessArticle

Toxicological Impacts of Polypropylene Nanoparticles Similar in Size to Nanoplastics in Plastic-Bottle Injections on Human Umbilical Vein Endothelial Cells

by Jie Wang, Zhong-Lan Chen, Cheng-Gang Liang, Hui-Ying Yang, Xian-Fu Wu and Hui-Min Sun

Toxics 2025, 13(9), 802; https://doi.org/10.3390/toxics13090802 - 21 Sep 2025

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Abstract

Microplastic and nanoplastic (MNP) particles have been observed in various human organs. However, polypropylene (PP), one of the top three most commonly detected types of MNPs in terms of quantity, is also present in injections given for the infusion treatment of diseases, and [...] Read more.

Microplastic and nanoplastic (MNP) particles have been observed in various human organs. However, polypropylene (PP), one of the top three most commonly detected types of MNPs in terms of quantity, is also present in injections given for the infusion treatment of diseases, and there is a considerable knowledge gap concerning its adverse effects on the human cardiovascular system. In this study, we used commercial PP particles (500 nm), similar in size to nanoplastics (NPs) present in injections and greater than or equal in concentration to NPs in the blood of healthy individuals, as the experimental dose to study their toxicological effects on human umbilical vein endothelial cells. The results revealed that PP particles at 35 μg/mL, equivalent to 20 times the concentration of blood, reduced cell viability, induced oxidative stress, caused cytomembrane damage, increased the inflammatory response, promoted apoptosis, and inhibited cell migration and wound tissue healing. In addition, a NP concentration of up to 210 μg/mL decreased the level of zonula occludens-1. In conclusion, since we used spherical particles, a type of nanoplastic present in plastic-bottle injections in clinical treatment that induces toxicological effects, this study provides cellular-level insights into the ecological risks of NP exposure in the human body. Full article

(This article belongs to the Special Issue Toxicity Assessment and Safety Management of Nanomaterials)

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11 pages, 231 KB

Open AccessArticle

DEET Metabolite and Hearing Loss in United States Adults

by Rae T. Benedict, Gregory M. Zarus, Franco Scinicariello, Henry G. Abadin and Roberta Attanasio

Toxics 2025, 13(9), 801; https://doi.org/10.3390/toxics13090801 - 20 Sep 2025

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Abstract

Hearing loss (HL) causes numerous challenges for individuals. N,N-diethyl-meta-toluamide (DEET) is a common ingredient in insect repellants and some sunscreens. M-(diethylcarbamoyl) benzoic acid (DCBA) is a DEET metabolite measured in the urine of National Health and Nutrition Examination Survey participants. This study examines [...] Read more.

Hearing loss (HL) causes numerous challenges for individuals. N,N-diethyl-meta-toluamide (DEET) is a common ingredient in insect repellants and some sunscreens. M-(diethylcarbamoyl) benzoic acid (DCBA) is a DEET metabolite measured in the urine of National Health and Nutrition Examination Survey participants. This study examines potential associations between HL and urinary DCBA in U.S. adults. Speech-frequency hearing loss (SFHL) was defined as an average hearing threshold above 25 dB across four frequencies (0.5, 1, 2, and 4 kHz). In comparison, high-frequency hearing loss (HFHL) was based on the average threshold above 25 dB at three higher frequencies (3, 4, and 6 kHz) in the better hearing ear. Hearing loss severity was categorized as normal (≤25 dB), mild (26–40 dB), and moderate or worse (≥41 dB). Higher DCBA exposure was significantly associated with increased odds of high-frequency hearing loss (HFHL). Participants in the highest exposure quartile had greater odds of HFHL (aOR = 2.34; 95% CI: 1.14–4.81), with a dose–response trend (p < 0.05) confirmed in sensitivity analyses controlling for chronic conditions and inflammation. Multinomial models further showed elevated odds of both slight/mild (aOR = 2.15; 95% CI: 1.05–4.42) and moderate/worse HFHL (aOR = 2.84; 95% CI: 1.10–7.37), supporting the robustness of the association. To our knowledge, this is the first report of HL being associated with a DEET metabolite in a nationally representative cross-sectional sample. Full article

(This article belongs to the Section Human Toxicology and Epidemiology)

23 pages, 2328 KB

Open AccessArticle

Constructed Wetlands with Novel Substrate Exposed to Nano-Plastics: Mitigating the Effects of Substrate Enzyme and Ecological Processes

by Luming Wang, Juan Huang, Jing Tuo, Jin Xu and Xinwei Li

Toxics 2025, 13(9), 800; https://doi.org/10.3390/toxics13090800 - 20 Sep 2025

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Abstract

The widespread occurrence of nano-plastics (NPs) in aquatic environments poses emerging challenges to the pollutant removal performance and ecological stability of constructed wetlands (CWs). This study investigates the performance of calcium-modified (Ca-MBF) and manganese-modified basalt fiber (Mn-MBF) bio-nests as novel substrates to mitigate [...] Read more.

The widespread occurrence of nano-plastics (NPs) in aquatic environments poses emerging challenges to the pollutant removal performance and ecological stability of constructed wetlands (CWs). This study investigates the performance of calcium-modified (Ca-MBF) and manganese-modified basalt fiber (Mn-MBF) bio-nests as novel substrates to mitigate NP-induced inhibition of CWs. Laboratory-scale CWs were operated for 180 days to evaluate substrate-associated enzyme activities, microbial community structure, and functional gene profiles. Results showed that Mn-MBF bio-nests enhanced the activities of dehydrogenase (DHA), urease (UR), ammonia monooxygenase (AMO), nitrite oxidoreductase (NOR), nitrate reductase (NAR), nitrite reductase (NIR), and phosphatase (PST) by 86.2%, 65.5%, 127.0%, 62.8%, 131.5%, 65.3%, and 107.0%, respectively, compared with the control. In contrast, Ca-MBF bio-nests increased these enzyme activities by 48.6%, 53.5%, 67.0%, 30.6%, 95.0%, 45.3%, and 54.6%, respectively. MBF bio-nests also enhanced microbial diversity, enriched denitrifying and phosphorus-removing bacteria (e.g., Thauera, Plasticicumulans), and promoted extracellular polymeric substance secretion. Functional gene prediction indicated elevated abundances of nitrogen cycle-related genes, thereby enhancing nitrification, denitrification, and phosphorus removal processes. These synergistic effects collectively improved nitrification, denitrification, and phosphorus removal efficiency, with Mn-MBF showing superior performance. This study highlights MBF bio-nests as a sustainable strategy to enhance the resilience and long-term operational stability of CWs in environments impacted by nano-plastic pollution. Full article

(This article belongs to the Special Issue Emerging Contaminants at the Water–Food–Energy–Climate Nexus: Exposure Pathways and Sustainable Mitigation)

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42 pages, 2650 KB

Open AccessReview

A Review of Quantitative Structure–Activity Relationship (QSAR) Models to Predict Thyroid Hormone System Disruption by Chemical Substances

by Marco Evangelista and Ester Papa

Toxics 2025, 13(9), 799; https://doi.org/10.3390/toxics13090799 - 19 Sep 2025

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Abstract

Thyroid hormone (TH) system disruption by chemicals poses a significant concern due to the key role the TH system plays in essential body functions, including the metabolism, growth, and brain development. Animal-based testing methods are resource-demanding and raise ethical issues. Thus, there is [...] Read more.

Thyroid hormone (TH) system disruption by chemicals poses a significant concern due to the key role the TH system plays in essential body functions, including the metabolism, growth, and brain development. Animal-based testing methods are resource-demanding and raise ethical issues. Thus, there is a recognised need for new approach methodologies, such as quantitative structure–activity relationship (QSAR) models, to advance chemical hazard assessments. This review, covering the scientific literature from 2010 to 2024, aimed to map the current landscape of QSAR model development for predicting TH system disruption. The focus was placed on QSARs that address molecular initiating events within the adverse outcome pathway for TH system disruption. A total of thirty papers presenting eighty-six different QSARs were selected based on predefined criteria. A discussion on the endpoints and chemical classes modelled, data sources, modelling approaches, and the molecular descriptors selected, including their mechanistic interpretations, was provided. By serving as a “state-of-the-art” of the field, existing models and gaps were identified and highlighted. This review can be used to inform future research studies aimed at advancing the assessment of TH system disruption by chemicals without relying on animal-based testing, highlighting areas that require additional research. Full article

(This article belongs to the Section Novel Methods in Toxicology Research)

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19 pages, 3517 KB

Open AccessArticle

Effects of Nitrogen and Phosphorus on Estuarine Phytoplankton Communities in Aquatic Microcosms

by Jianan Ling, Chao Wei, Dongning Yang, Jiangning Zeng, Fangping Cheng, Xin Zheng and Zhanhong Yang

Toxics 2025, 13(9), 798; https://doi.org/10.3390/toxics13090798 - 19 Sep 2025

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Abstract

Phytoplankton serves as the primary producer in estuarine ecosystems, with its community structure and dynamics being directly influenced by the concentration and ratio of nitrogen (N) and phosphorus (P) nutrients. This study utilized raw water from the Yangtze Estuary to establish a series [...] Read more.

Phytoplankton serves as the primary producer in estuarine ecosystems, with its community structure and dynamics being directly influenced by the concentration and ratio of nitrogen (N) and phosphorus (P) nutrients. This study utilized raw water from the Yangtze Estuary to establish a series of ocean microcosm systems, setting up gradients of dissolved inorganic nitrogen (DIN) and reactive phosphate (SRP) concentrations to explore the reaction of phytoplankton communities over 30 days. The results indicated that total phytoplankton abundance significantly increased under prolonged exposure to high concentrations of DIN and SRP. However, the community diversity indices exhibited a declining tendency, indicating a simplification and increased instability of the community structure. Diatoms and dinoflagellates, the predominant phytoplankton taxa, differed in their response to DIN and SRP. Diatom abundance rose at elevated DIN concentrations and initially increased and then decreased at high SRP concentrations, while dinoflagellate abundance diminished at high DIN concentrations and persisted in increasing at elevated SRP concentrations. An ecological threshold is the critical point at which the structure or function of an ecosystem undergoes significant changes when subjected to external disturbances or internal changes. The Threshold Indicator Taxa Analysis (TITAN) was employed to identify indicator species within the microcosm systems, revealing that the ecological response thresholds of phytoplankton communities to DIN and SRP were 0.50 mg/L and 0.030 mg/L, respectively. This study quantitatively analyzed the environmental exposure concentrations of DIN and SRP at the community level and calculated the ecological response thresholds, providing fundamental data and a scientific basis for nitrogen and phosphorus management in estuaries. Full article

(This article belongs to the Section Ecotoxicology)

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16 pages, 5677 KB

Open AccessArticle

The Effect of Radon Concentration on MS Prevalence: A Door-to-Door Survey in the Fault Zone in Afyonkarahisar, Turkey

by Ülkü Türk Börü, Ahmet Yıldız, Metin Bağcı, Ayla Sandıkçıoğlu Gümüş, Elif Simin Issı, Furkan İncebacak, Hakan Acar and Cem Bölük

Toxics 2025, 13(9), 797; https://doi.org/10.3390/toxics13090797 - 19 Sep 2025

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Abstract

Background: Despite the identification of various environmental factors that increase the risk of multiple sclerosis (MS), the effects of many factors on the etiology of MS remain to be elucidated. In this study, we aimed to investigate the effects of radon, a factor [...] Read more.

Background: Despite the identification of various environmental factors that increase the risk of multiple sclerosis (MS), the effects of many factors on the etiology of MS remain to be elucidated. In this study, we aimed to investigate the effects of radon, a factor previously studied in relation to various other neurodegenerative diseases, on the epidemiology of MS. Methods: A door-to-door field study was conducted in residential areas with relatively high and low radon gas concentrations to determine the prevalence of MS. The study area comprises the Bolvadin and İhsaniye regions, which have different geological characteristics, such as seismic activity, active faults, and distributions of volcanic rocks. CR-39 detectors, with an accepted limit of 300 Bq/m³, were utilized to measure radon gas concentrations. During the screening field, the patients diagnosed with multiple sclerosis were confirmed with their hospital records. Mc Donald’s revised diagnostic criteria were used for multiple sclerosis diagnosis. Results: The regions were grouped into higher radon areas and lower radon areas. The İhsaniye city center, Kayıhan, Kemerkaya, Döğer, and Bolvadin city center were classified as higher radon regions, whereas Dişli, Yaylabağı, Gazlıgöl, and Özburun were identified as lower radon regions. A total of 40,841 individuals were surveyed in the field. The crude MS prevalence was 41.8/100,000 in settlements with high radon gas concentrations and 20.5/100,000 in settlements with low radon gas concentrations. Conclusions: In this study, we revealed that the prevalence of MS was greater in settlements with high radon gas concentrations than in settlements with low radon gas concentrations. These results demonstrated that radon gas is an important environmental risk factor in the etiopathogenesis of MS. Full article

(This article belongs to the Special Issue Neuronal Injury and Disease Induced by Environmental Toxicants)

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13 pages, 703 KB

Open AccessArticle

Health Symptoms Related to Polycyclic Aromatic Hydrocarbon (PAH) Exposure in Chiang Mai, Thailand: Associations with Biomarkers of Exposure and Oxidative Stress

by Xianfeng Cao, Sumed Yadoung, Phannika Tongchai, Anurak Wongta, Kanokwan Kulprachakarn, Peerapong Jeeno, Pichamon Yana, Udomsap Jaitham, Wenting Li, Kai Zhou, Xiao Zhang, Jianmei Gong, Natthapol Kosashunhanan and Surat Hongsibsong

Toxics 2025, 13(9), 796; https://doi.org/10.3390/toxics13090796 - 18 Sep 2025

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Abstract

Northern Thailand experiences seasonal surges in PM₂.₅ pollution, posing significant respiratory health risks. This cross-sectional study aimed to evaluate associations between PAHs exposure and early health biomarkers. In April 2024, 127 rural residents in Chiang Mai were recruited during a [...] Read more.

Northern Thailand experiences seasonal surges in PM₂.₅ pollution, posing significant respiratory health risks. This cross-sectional study aimed to evaluate associations between PAHs exposure and early health biomarkers. In April 2024, 127 rural residents in Chiang Mai were recruited during a high-exposure period (mean monthly PM_2.5 = 41.7 μg/m³). Participants reporting eye irritation and pneumonia showed significantly higher 8-iso-PGF₂α levels (p = 0.010 and 0.012, respectively). Smokers exhibited elevated CC16 levels (130.0 ± 65.3 ng/mL) compared to non-smokers (96.3 ± 39.9 ng/mL, p < 0.05). CC16 was also significantly associated with self-reported symptoms, including fatigue, poor sleep quality, and activity limitation. For example, participants who reported difficulty performing daily activities (i.e., disagreed with the statement “I can do things at home without any restrictions”) had significantly higher CC16 levels (108 ± 47 ng/mL) than those without such limitations (74 ± 35 ng/mL; p < 0.001). A weak but significant positive correlation was observed between respiratory rate and CC16 (R² = 0.334, p = 0.001). Interestingly, serum 8-iso-PGF₂α was inversely associated with diabetes (OR = 0.965; 95% CI: 0.935–0.997; p = 0.033), potentially indicating a compensatory or phenotype-specific oxidative stress response. In addition, CC16 levels were positively associated with diabetes (p = 0.022), suggesting altered epithelial responses in individuals with metabolic disease. CC16 and 8-iso-PGF₂α demonstrated significant associations with respiratory symptoms and metabolic status, suggesting their potential as early indicators for environmental health surveillance in haze-affected populations. Full article

(This article belongs to the Special Issue The Effect of Airborne Contaminants Exposure on Early Health Damage Biomarkers: 2nd Edition)

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18 pages, 1736 KB

Open AccessArticle

Insights into How Degradable Microplastics Enhance Cu²⁺ Mobility in Soil Through Interfacial Interaction

by Hongjia Peng, Bolun Yu, Zuhong Lin and Haipu Li

Toxics 2025, 13(9), 795; https://doi.org/10.3390/toxics13090795 - 18 Sep 2025

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Abstract

The incomplete degradation of degradable plastics may pose potential ecological risks, as it can generate degradable microplastics (DMPs), especially when these DMPs coexist with heavy metals in soil. Taking petrochemical-based poly(butylene adipate-co-terephthalate) (PBAT) and bio-based polylactic acid (PLA) as representative DMPs, this study [...] Read more.

The incomplete degradation of degradable plastics may pose potential ecological risks, as it can generate degradable microplastics (DMPs), especially when these DMPs coexist with heavy metals in soil. Taking petrochemical-based poly(butylene adipate-co-terephthalate) (PBAT) and bio-based polylactic acid (PLA) as representative DMPs, this study investigated how DMPs affect the adsorption–desorption behavior of Cu²⁺ in soil and the underlying mechanisms via batch equilibrium experiments and characterization analyses. The experiments revealed that ion exchange (accounting for 33.6–34.3%), oxygen-containing functional group complexation, and electrostatic interactions were the primary adsorption driving forces, with chemical adsorption playing the main role. Compared to the soil, the PBAT and PLA had smaller specific surface areas and pore volumes, fewer oxygen-containing functional groups, and especially lacked O-metal functional groups. They can dilute soil, clog its pores, and cover its active sites. 1% DMPs significantly reduced the soil’s equilibrium adsorption capacity (Q_e) (3.7–4.7%) and increased equilibrium desorption capacity (Q_De) (1.7–2.6%), thereby increasing the mobility and ecological risk of Cu²⁺. PBAT and PLA had no significant difference in effects on the adsorption, but their specific mechanisms were somewhat distinct. Faced with the prevalent, worsening coexistence of DMPs and heavy metals in soil, these findings contribute to the ecological risk assessment of DMPs. Full article

(This article belongs to the Special Issue Nano-Toxicity: Exploring the Ecotoxicological Impacts of Nanoparticles and Combined Exposures)

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14 pages, 1427 KB

Open AccessFeature PaperArticle

Modulation of Detoxification, Immune, and Epigenetic Systems by Two Aryl Organophosphorus Flame Retardants During Early Development in Zebrafish

by Montserrat Solé, Sílvia Joly, Sergi Omedes, Isabel Forner-Piquer and Laia Ribas

Toxics 2025, 13(9), 794; https://doi.org/10.3390/toxics13090794 - 18 Sep 2025

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Abstract

Organophosphorus flame retardants (OPFRs) are emerging alternatives to halogenated compounds, yet their environmental toxicity remains underexplored. This study evaluated the developmental toxicity of two aryl-OPFRs, triphenyl phosphate (TPP) and tricresyl phosphate (TCP), in zebrafish (Danio rerio) from 2 h to 5 [...] Read more.

Organophosphorus flame retardants (OPFRs) are emerging alternatives to halogenated compounds, yet their environmental toxicity remains underexplored. This study evaluated the developmental toxicity of two aryl-OPFRs, triphenyl phosphate (TPP) and tricresyl phosphate (TCP), in zebrafish (Danio rerio) from 2 h to 5 days post fertilization (hpf–dpf). Survival, hatching rate, and malformations were assessed across concentrations of 250–1000 µg/L, alongside with gene expression analysis at 5 dpf (250 and 500 µg/L) targeting detoxification (ces2), immune responses (il1β, casp9), and epigenetic markers (dnmt1, dnmt3). In vitro enzymatic assays evaluated interactions of both aryl-OPFRs with carboxylesterase (CE) and acetylcholinesterase (AChE) enzymes. While no significant morphological effects were observed, TPP showed higher toxicity than TCP. Notably, TCP (500 µg/L) downregulated genes linked to metabolism and immunity. CE activity and ces2 modulation may suggest CE as a potential biomarker for aryl-OPFR exposure. These findings, although at concentrations above the environmental ones, may be valuable for mechanistic purposes and underscore the need for further investigation in developmental toxicity given their lipophilic nature and distinct molecular responses. Full article

(This article belongs to the Special Issue Hazardous Pollutants in Marine Ecosystems)

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14 pages, 631 KB

Open AccessArticle

A Comparative Study of the Effect of Commonly Used Pesticides on Cervical Contractions in Pregnant Cows, In Vitro

by Michal Hubert Wrobel

Toxics 2025, 13(9), 793; https://doi.org/10.3390/toxics13090793 - 17 Sep 2025

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Abstract

Organochlorine insecticides (DDTs), organophosphate insecticides (malathion), carbamate insecticides (carbaryl and thiram), pyrethroid (cypermethrin and fenvalarate) insecticides, and herbicides (glyphosate and atrazine) were selected for this study because they disrupt cervical and myometrial function in the bovine oestrous cycle. However, their potential to affect [...] Read more.

Organochlorine insecticides (DDTs), organophosphate insecticides (malathion), carbamate insecticides (carbaryl and thiram), pyrethroid (cypermethrin and fenvalarate) insecticides, and herbicides (glyphosate and atrazine) were selected for this study because they disrupt cervical and myometrial function in the bovine oestrous cycle. However, their potential to affect reproductive success in cattle during pregnancy has not been directly confirmed. The aim of this study was to determine the effects of the investigated pesticides on cervical contractions in pregnant cows. Cervical strips from cows at 4–6 months of gestation were treated with the eight singular pesticides (used at non-toxic, environmental dose) or oestradiol (E2) under two different conditions (37.5 °C for 24 h and 4 °C for 48 h), which were applied to assess pesticide effects under both physiological and prolonged-exposure settings. The strength of the contractions was then measured. The findings of the study demonstrated that both the carbamates and glyphosate increased the force of cervical strip contractions to a greater extent than cypermethrin. In contrast, fenvalerate was observed to induce a state of cervical relaxation, analogous to the effects of E2, while DDT, malathion and atrazine exerted no effect on cervical motor function during the period of pregnancy under investigation. These preliminary findings indicate a potential impact of pesticides on cervical function during pregnancy, but should be interpreted with caution as they are based on isolated tissue at a single concentration. Further in vivo and dose–response studies are needed to confirm their biological and clinical relevance. Full article

(This article belongs to the Special Issue Endocrine-Disrupting Chemicals and Reproductive Toxicology)

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10 pages, 402 KB

Open AccessArticle

Environmental Exposure to Waterborne Pollutants and Colorectal Cancer Risk in Lebanon

by Magie Tamraz, Najib Al Ghossaini and Sally Temraz

Toxics 2025, 13(9), 792; https://doi.org/10.3390/toxics13090792 - 17 Sep 2025

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Abstract

Colorectal cancer (CRC) has a complex etiology involving genetic, lifestyle, and environmental factors. This study investigates the association between exposure to water contaminants and the risk of developing CRC in Lebanon. Population Attributable Fraction (PAF) was calculated using exposure prevalence from environmental reports [...] Read more.

Colorectal cancer (CRC) has a complex etiology involving genetic, lifestyle, and environmental factors. This study investigates the association between exposure to water contaminants and the risk of developing CRC in Lebanon. Population Attributable Fraction (PAF) was calculated using exposure prevalence from environmental reports and water quality assessments. Relative risks (RR) were obtained from multiple meta-analyses and epidemiological studies to quantify the contribution of five water contaminants to CRC incidence: nitrates, mercury, arsenic, trihalomethanes (THMs), and microbial pollutants (E. coli). A synthetic cohort was simulated using national exposure data and probabilistic techniques, employing multivariate logistic regression models to ensure precise CRC risk and controlling for lifestyle covariates. Adjusted PAF values were calculated using regression data to determine the proportion of CRC cases attributable to each water contaminant. THMs and E. coli showed the strongest association with CRC, with adjusted PAF values of 25.76% and 23.65%, respectively. Exposure to nitrates, arsenic, and mercury contributed less to CRC risk (1.02%, 0.52% and 2.20%, respectively). Multivariate regression confirmed that THMs and E. coli remained the strongest independent predictors of CRC risk (OR = 1.37, p = 0.001) and (OR = 1.79, p < 0.0001) among water contaminants, respectively. Our model-based approach carries limitations due to reliance on indirect exposure and risk estimates, which may introduce uncertainty stemming from data gaps and underlying assumptions. This study highlights the importance of water quality management in CRC prevention as exposure to drinking water contaminants contributes meaningfully to disease burden. Full article

(This article belongs to the Section Exposome Analysis and Risk Assessment)

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16 pages, 4482 KB

Open AccessArticle

Unveiling the Distribution Characteristics of Benzene-Based Pollutants in a Retired Industrial Park and Their Influence Factors: Soil Properties and Microbial Communities

by Lei Wang, Weizhen Chen, Xuejun Tan and Li Xie

Toxics 2025, 13(9), 791; https://doi.org/10.3390/toxics13090791 - 17 Sep 2025

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Abstract

With the transformation of industrial enterprises in China, the relocation of numerous factories has led to the emergence of retired industrial parks with serious pollution. This study investigated the contamination of benzene-based pollutants (BBPs) in soil and their relationship with soil texture, physicochemical [...] Read more.

With the transformation of industrial enterprises in China, the relocation of numerous factories has led to the emergence of retired industrial parks with serious pollution. This study investigated the contamination of benzene-based pollutants (BBPs) in soil and their relationship with soil texture, physicochemical properties, and microbial communities at a former factory site in Shanghai. The results indicated that benzene and toluene were the main pollutants in the region, accounting for 25.7–36.1% and 7.6–10.2% of the total pollutants, respectively. The horizontal contamination distribution pattern of BBPs at different sampling points were clearly related to the functional zoning of the area. Sampling points close to workshops and bathrooms possessed higher contamination levels of BBPs than those close to warehouses and office buildings. With the increase in sampling depth, the gradually rising soil density and soil porosity ratio reduced the adsorption capacity of soil for BBPs, thereby promoting the volatilization and release of BBPs in deeper soil layers to a certain extent, resulting in a “shallow > deep” trend for the content of BBPs. The abundance of norank_f__norank__o_norank__c__Bathyarchaeia in the soil may be the main functional microorganisms affecting the distribution of BBPs. Styrene and chlorobenzene exhibited significant correlations with microbial communities, primarily involving bacteria (Desulfobacterium, Thermincola, and Trichlorobacter) and archaea (including norank_f_Nitrosopumilaceae, norank_f_norank_o_norank_c_Nitrososphaeria, and Methanocella). This study identifies and analyzes the BBP contamination characteristics in a typical retired industrial park in Shanghai, providing valuable references for risk assessment and microbial remediation of such contaminated areas. Full article

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19 pages, 1265 KB

Open AccessReview

In Silico Forensic Toxicology: Is It Feasible?

by Ivan Šoša

Toxics 2025, 13(9), 790; https://doi.org/10.3390/toxics13090790 - 17 Sep 2025

Viewed by 384

Abstract

In silico forensic toxicology refers to the emerging application of computational models based on Quantitative Structure–Activity Relationships (QSARs), molecular docking, and predictions regarding Absorption, Distribution, Metabolism, Excretion, and Toxicity (ADMET) as used to predict the toxicological behavior of various substances, particularly in medico-legal [...] Read more.

In silico forensic toxicology refers to the emerging application of computational models based on Quantitative Structure–Activity Relationships (QSARs), molecular docking, and predictions regarding Absorption, Distribution, Metabolism, Excretion, and Toxicity (ADMET) as used to predict the toxicological behavior of various substances, particularly in medico-legal contexts. These computational models replicate metabolic pathways, providing insights into the metabolism of substances in the human body, while the results of this approach effectively reflect the necessary compounds, reducing the need for direct laboratory work. This review aims to evaluate whether forensic settings and in silico methods present a cost-effective strategy for investigating unknown substances, aiding in toxicological interpretations, and steering laboratory process analyses. Additionally, financial considerations, such as break-even analysis and Bland–Altman plots, were conducted, indicating that forensic labs conducting over 625 analyses each year can achieve cost efficiency by integrating in silico strategies, thus making them a viable alternative to conventional methods in high-throughput settings. Recent studies have emphasized how machine learning enhances predictive accuracy, thereby boosting forensic toxicology’s capacity to effectively evaluate toxicity endpoints. In silico methods are essential for cases involving novel psychoactive substances (NPSs) or unclear toxicological findings. They are also useful as a supporting method in legal contexts, as they uphold expert testimonies and reinforce evidence claims. The future of forensic toxicology is likely to see the increased implementation of AI-powered techniques, streamlining toxicological investigations and enhancing overall accuracy in forensic evaluations. Full article

(This article belongs to the Collection Predictive Toxicology)

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11 pages, 274 KB

Open AccessBrief Report

Examination of DNA Methylation Patterns in Children Born Premature with Prenatal Tobacco Smoke Exposure

by Olivia E. Gittens, Alonzo T. Folger, Xue Zhang, Lili Ding, Nehal A. Parikh and E. Melinda Mahabee-Gittens

Toxics 2025, 13(9), 789; https://doi.org/10.3390/toxics13090789 - 17 Sep 2025

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Abstract

Prenatal tobacco smoke exposure (TSE) has been associated with significant alterations in DNA methylation (DNAm), an epigenetic mechanism with potential functional consequences to child development. This pilot study aimed to investigate differential DNAm patterns in preterm children with and without prenatal TSE using [...] Read more.

Prenatal tobacco smoke exposure (TSE) has been associated with significant alterations in DNA methylation (DNAm), an epigenetic mechanism with potential functional consequences to child development. This pilot study aimed to investigate differential DNAm patterns in preterm children with and without prenatal TSE using reduced representation bisulfite sequencing (RRBS) to interrogate a wider array of sites than in more common approaches, namely microarrays. Buccal swabs were collected from 16 two-year-old children (7 with TSE, 9 without), and DNAm was quantified at over 1.3 million CpG sites. To identify differential DNAm, univariable analyses were first performed and followed by Bayesian beta-binomial hierarchical regression models for sequence count data including adjustment for potential confounders. False Discovery Rate correction was used to account for multiple comparisons. Significant differential methylation was observed at CpG sites within intronic regions of the CALN1 and LINGO1 genes and the distal intergenic region of the TBL1XR1 gene. These findings suggest that prenatal TSE may influence epigenetic regulation in genes involved in neurodevelopment. This study demonstrates the importance of RRBS in identifying novel DNAm changes associated with prenatal TSE and highlights the need for larger studies to validate and expand upon these preliminary findings. Full article

(This article belongs to the Special Issue Environmental Contaminants and Human Health—2nd Edition)

14 pages, 1759 KB

Open AccessReview

Lung Deposition of Particulate Matter as a Source of Metal Exposure: A Threat to Humans and Animals

by Joel Henrique Ellwanger, Marina Ziliotto and José Artur Bogo Chies

Toxics 2025, 13(9), 788; https://doi.org/10.3390/toxics13090788 - 17 Sep 2025

Viewed by 248

Abstract

The deposition of particulate matter (PM) in the lungs is a health problem that primarily affects individuals working in mines and other highly polluted environments. People living in large cities also accumulate PM in their lungs throughout their lives due to the high [...] Read more.

The deposition of particulate matter (PM) in the lungs is a health problem that primarily affects individuals working in mines and other highly polluted environments. People living in large cities also accumulate PM in their lungs throughout their lives due to the high levels of air pollution often observed in urban environments. In addition to the direct effects that the physical deposition of PM causes in the lungs, such as increased levels of inflammation and fibrosis, these pollutants can be associated with additional toxic effects, including genotoxicity and other molecular, cellular, and systemic alterations that can lead to the development of multiple diseases. This occurs because PM carries a number of toxic pollutants to the lungs, especially metals and metalloids such as arsenic, lead, cadmium, chromium, and mercury. Although the histopathological effects of occupational (pneumoconiosis) or environmental (anthracosis) deposition of PM in the human lungs are well described, little is discussed about how these morphological alterations can be a proxy for acute and chronic exposure to several toxic metals. Furthermore, the effects of PM–metal complexes on the health of animals, especially those living in urban environments, are often overlooked. In this context, this narrative review aims to discuss the impacts of lung-deposited PM–metal complexes on the health of multiple species, highlighting the broad effects caused by air pollution. Using the One Health approach, this article examines how environmental issues impact the health of both humans and animals. Full article

(This article belongs to the Special Issue Heavy Breathing: Unveiling the Impact of Heavy Metals on Lung Health)

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19 pages, 2358 KB

Open AccessArticle

Synergy of Arsenic and Graphene Oxide in Utero and Lactation Exacerbates Reproductive Disorders in Female Rat Offspring Undergoing Puberty and Maturity

by Reda H. ElMazoudy, Azza A. Attia and Tawfik A. Saleh

Toxics 2025, 13(9), 787; https://doi.org/10.3390/toxics13090787 - 17 Sep 2025

Viewed by 413

Abstract

Notably, the widespread ubiquity of arsenic and graphene oxide in the environment validates the occurrence of their co-exposure, posing significant threats to target organisms, including humans. Herein, prepuberty, puberty, and maturity were investigated using anogenital distance, vaginal opening, first estrus, reproductive hormone profiles, [...] Read more.

Notably, the widespread ubiquity of arsenic and graphene oxide in the environment validates the occurrence of their co-exposure, posing significant threats to target organisms, including humans. Herein, prepuberty, puberty, and maturity were investigated using anogenital distance, vaginal opening, first estrus, reproductive hormone profiles, cyclicity, sexual behaviour and pregnancy outcomes to assess the impact of exposure to arsenic and/or graphene oxide on the puberty of offspring female rats after maternal exposure during gestation and lactation periods. Zero-day pregnant Sprague Dawley females were randomly divided into four groups, each receiving a different treatment via drinking water from gestation day 0 to postnatal day 21: control group (CON, drinking water); arsenic group (ARS, 10 mg/L sodium arsenite); graphene oxide group (GOX, 0.5 mg/mL); and co-exposure group (ARS + GOX; 10 mg/L of arsenic combined with 0.5 mg/mL of graphene oxide). Individually or combined, arsenic and graphene oxide exposure increase the sexual retardation and female masculinization, as evidenced by a significant increase in anogenital distance, delay in the first estrus cycle, and prolongation in the timing of the vaginal opening. At maturity, the offspring exhibited a significant elevation of testosterone and a significant decrease in estradiol. Offspring females showed inhibited receptivity to their male mates, indicated by lower lordosis quotient and intensity. Additionally, there was an increase in the number of estrous cycles but a decrease in their duration. Moreover, an increase in implantation loss and the number of resorbed embryos, along with a reduction in viable fetuses. In conclusion, reproductive deterioration was more significant in the offspring exposed to combined arsenic and graphene oxide compared to those exposed to ARS or GOX alone, indicating that arsenic exposure is exacerbated when combined with graphene oxide during the experimental episode. Full article

(This article belongs to the Section Reproductive and Developmental Toxicity)

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14 pages, 2587 KB

Open AccessArticle

Mercury Exposure, Gene Expression, and Intelligence Quotient in Afro-Descendant Children from Two Colombian Regions

by Javier Galvis-Ballesteros, Margareth Duran-Izquierdo, Juan Valdelamar-Villegas, Lucellys Sierra-Marquez and Jesus Olivero-Verbel

Toxics 2025, 13(9), 786; https://doi.org/10.3390/toxics13090786 - 17 Sep 2025

Viewed by 1105

Abstract

The impact of mercury (Hg) on biological systems is well documented; however, the long-term effects of low-level exposure in children remain unclear, particularly with respect to oxidative stress and cognitive outcomes. This study evaluated Hg exposure and its associations with the gene expression [...] Read more.

The impact of mercury (Hg) on biological systems is well documented; however, the long-term effects of low-level exposure in children remain unclear, particularly with respect to oxidative stress and cognitive outcomes. This study evaluated Hg exposure and its associations with the gene expression and intelligence quotient (IQ) in two Afro-descendant child populations in Colombia. Hair total mercury (T-Hg) was quantified in 163 children under 7 years old, along with their sociodemographic data. Significant differences (p < 0.05) were found in fish consumption and mean hair T-Hg concentrations between children from Mahates (2.66 ± 0.30 meals/week; 0.32 ± 0.03 µg/g) and Zanjón (1.24 ± 0.09 meals/week; 0.24 ± 0.01 µg/g). The gene expression analysis revealed higher SOD1 expression in Mahates. The mean IQ scores were higher in Zanjón (74.7) than those in Mahates (71.7). Overall, Spearman’s correlation analysis showed no significant associations (p > 0.05) between T-Hg and the measured variables. The principal component analysis (PCA) revealed a clear separation between populations: Mahates, associated with a higher mercury burden and the upregulation of stress-response genes, and Zanjón, characterized by a better cognitive performance and lower mercury exposure. These findings suggest that despite the low overall exposure and non-significant bivariate correlations, the communities displayed distinct profiles, highlighting the value of integrated molecular–cognitive biomonitoring and motivate longitudinal studies addressing co-exposures and socioeconomic confounding. Full article

(This article belongs to the Section Human Toxicology and Epidemiology)

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13 pages, 456 KB

Open AccessArticle

Chronic Occupational Exposure to Chemical Mixtures Induces Genomic Instability in Paint Workers

by Servet Birgin İritaş, Merve Güdül Bacanlı, Gökçe Taner, Vugar Ali Türksoy, Lütfiye Tutkun, Ömer Hınç Yilmaz and Ayşe Nurşen Başaran

Toxics 2025, 13(9), 785; https://doi.org/10.3390/toxics13090785 - 17 Sep 2025

Viewed by 341

Abstract

This study’s objective was to evaluate genotoxic effects on automotive paint workers who are exposed to a complex mixture of VOCs, heavy metals, and solvents. Biological samples, including blood, urine, and buccal epithelial cells, were collected from 80 exposed workers and 80 demographically [...] Read more.

This study’s objective was to evaluate genotoxic effects on automotive paint workers who are exposed to a complex mixture of VOCs, heavy metals, and solvents. Biological samples, including blood, urine, and buccal epithelial cells, were collected from 80 exposed workers and 80 demographically matched control subjects. DNA damage was assessed using the alkaline COMET assay in lymphocytes and whole blood. The Buccal Micronucleus Cytome (BMCyt) assay was also employed to identify cytogenetic abnormalities. Additionally, trichloroacetic acid (TCA), hippuric acid (HA), phenol, and lead (Pb) levels were measured as biomarkers of exposure. A significant increase in DNA damage was observed in the lymphocytes and whole blood of exposed workers (p < 0.05) BMCyt analysis also revealed higher frequencies of micronuclei (MN), binucleated cells, condensed chromatin (CC), and karyorrhectic (KHC) and pyknotic cells (PYC) in buccal cells (p < 0.05). Elevated levels of urinary HA, phenol, TCA, and blood lead indicated systemic chemical exposure. DNA damage positively correlated with these biomarkers, supporting a strong link between chronic occupational exposure and genotoxicity. The findings from this study highlight the critical importance of implementing effective safety measures and consistent biomonitoring for paint workers to prevent adverse health effects. Full article

(This article belongs to the Section Exposome Analysis and Risk Assessment)

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23 pages, 2703 KB

Open AccessArticle

Ametryn and Clomazone Disrupt Mitochondrial Bioenergetics in Rat Liver: Evidence for Inhibition of Complexes I and II and ATP Synthase

by Heberth Paulo dos Santos Silva, Camila Ortiz, Camila Araújo Miranda, Paulo Francisco Veiga Bizerra, Carlos Manuel Palmeira and Fábio Erminio Mingatto

Toxics 2025, 13(9), 784; https://doi.org/10.3390/toxics13090784 - 16 Sep 2025

Viewed by 234

Abstract

Ametryn (AMT) and clomazone (CLZ) are commonly used herbicides frequently detected in food and water, raising concerns about potential health risks. This study investigated whether AMT and CLZ impair mitochondrial bioenergetics, a key mechanism linked to hepatotoxicity. Mitochondria were isolated from rat liver [...] Read more.

Ametryn (AMT) and clomazone (CLZ) are commonly used herbicides frequently detected in food and water, raising concerns about potential health risks. This study investigated whether AMT and CLZ impair mitochondrial bioenergetics, a key mechanism linked to hepatotoxicity. Mitochondria were isolated from rat liver and incubated with AMT or CLZ (50–200 µM) to assess respiration, membrane potential (Δψ), ATP production, and the activities of respiratory chain complexes and ATP synthase. Both herbicides significantly inhibited state 3 (ADP-stimulated) respiration with glutamate plus malate, without altering state 4 (basal) respiration. Concentrations above 100 µM reduced Δψ and ATP synthesis in glutamate plus malate or succinate-energized mitochondria. Enzymatic assays revealed inhibition of complex I by both herbicides, complex II by CLZ, and ATP synthase by both. These results highlight mitochondrial oxidative phosphorylation disruption by AMT and CLZ; however, further in situ and in vivo studies are necessary to fully understand their hepatotoxic potential. Full article

(This article belongs to the Section Agrochemicals and Food Toxicology)

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21 pages, 6258 KB

Open AccessArticle

Ecological and Human Health Risk Assessment of Heavy Metals in Mining-Affected River Sediments in the Peruvian Central Highlands

by María Custodio, Samuel Pizarro, Javier Huarcaya, Kevin Ortega and Dennis Ccopi

Toxics 2025, 13(9), 783; https://doi.org/10.3390/toxics13090783 - 16 Sep 2025

Viewed by 586

Abstract

Heavy metal contamination in rivers is a serious environmental and public health concern, especially in areas affected by mining. This study evaluated the levels of contamination and the associated ecological and carcinogenic risks in the sediments of the Cunas River, located in the [...] Read more.

Heavy metal contamination in rivers is a serious environmental and public health concern, especially in areas affected by mining. This study evaluated the levels of contamination and the associated ecological and carcinogenic risks in the sediments of the Cunas River, located in the central highlands of Peru. Sediment samples were collected from upstream and downstream sections. Several metals and metalloids were analyzed, including copper (Cu), chromium (Cr), iron (Fe), manganese (Mn), molybdenum (Mo), nickel (Ni), lead (Pb), vanadium (V), zinc (Zn), antimony (Sb), arsenic (As), and cadmium (Cd). The ecological risk assessment focused on ten of these elements, while carcinogenic and non-carcinogenic risks were assessed for seven metals selected based on their toxicological importance. The results showed that Cd and Pb concentrations were higher in the downstream section. Cd and As exceeded ecological risk thresholds. Regarding human health, As and Pb surpassed the acceptable limits for both the Hazard Index (HI) and the Potential Carcinogenic Risk (PCR). According to EPA guidelines, these values indicate a potentially significant lifetime cancer risk. The main exposure routes include direct contact with sediments and the consumption of aquatic organisms. Continuous monitoring, phytoremediation actions, and restrictions on the use of contaminated water are strongly recommended to reduce ecological and health risks. Full article

(This article belongs to the Special Issue Health Risk Assessment of Exposure to Emerging Contaminants)

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19 pages, 1090 KB

Open AccessReview

Microplastics in Drinking Water: A Review of Sources, Removal, Detection, Occurrence, and Potential Risks

by Ting Cai, Zhihe Tang, Tao Gu, Kun Tong, Xinwei Wang, Hao Chen, Xingnan Zhou, Zi Long, Chunmei Hao, Chunmao Chen and Rong Zeng

Toxics 2025, 13(9), 782; https://doi.org/10.3390/toxics13090782 - 15 Sep 2025

Viewed by 911

Abstract

The emergence of microplastics (MPs) in drinking water supply systems has become a significant environmental challenge. Although the potential impacts of MPs in drinking water on human health remain incompletely understood, the ingestion of MPs through drinking water has raised substantial public concern [...] Read more.

The emergence of microplastics (MPs) in drinking water supply systems has become a significant environmental challenge. Although the potential impacts of MPs in drinking water on human health remain incompletely understood, the ingestion of MPs through drinking water has raised substantial public concern regarding health risks. This review synthesizes contemporary scientific advances focusing on the following: I. the sources and fate of MPs in drinking water supply chains; II. comparative assessment of removal at treatment; III. detection techniques based on microscopy, spectroscopic, and thermal methods; and IV. the potential hazards of MPs to human health. This study aims to provide novel insights into understanding the threats posed by MPs in drinking water and to facilitate the development of effective monitoring strategies. Full article

(This article belongs to the Special Issue Identification of Emerging Pollutants and Human Exposure)

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16 pages, 5363 KB

Open AccessArticle

Neglected Microplastics and Their Risks in Rivers Throughout the Three Gorges Reservoir Area

by Jian-Yun Xie, Bo Li, Qun-Po Jia, Xiao Li, Qin Zhao, Pei-Dang Fan, Chang-Qing Wang and Liu-Yi Zhang

Toxics 2025, 13(9), 781; https://doi.org/10.3390/toxics13090781 - 15 Sep 2025

Viewed by 262

Abstract

Microplastics have become a global environmental issue, and this challenge has also emerged in river environments. In this study, the Three Gorges Reservoir (TGR) was selected as the research area, and microplastics in water and sediments were chosen as the objects. The occurrence [...] Read more.

Microplastics have become a global environmental issue, and this challenge has also emerged in river environments. In this study, the Three Gorges Reservoir (TGR) was selected as the research area, and microplastics in water and sediments were chosen as the objects. The occurrence characteristics of microplastics were presented in rivers of the TGR through the methods of field sample collection and laboratory testing, and the risks of microplastics were evaluated using the pollution risk index (PRI) and potential ecological risk index. The results showed that the average abundances of microplastics in water and sediments of the rivers from the TGR were 15,464 particles/m³ and 1838 particles/kg, respectively. Furthermore, the main colors of microplastics in water and sediments of the TGR were black and blue, and the particle sizes were concentrated in the range of 200 to 500 μm. Polypropylene and polyethylene were the major polymers of microplastics in the water of the TGR, while the polypropylene–polyethylene copolymer was dominant in sediments. The PRIs in the water of the Yangtze River and its tributary from the TGR area were 2.45 and 1.72, respectively, and their PRIs were 2.43 and 2.01 in sediments, respectively, showing a low risk of pollution (level I). The average comprehensive potential ecological risk indices (RIs) for the Yangtze River and its tributaries were 27.28 and 18.82 in the water of the TGR, respectively, indicating low and moderate risk grades. However, there was a significantly high risk in sediment, and the average RI was 130.57 for the Yangtze River and 70.05 for the tributaries. Importantly, the neglected risks of microplastics in the river environment of the TGR area have been revealed, which provides a critical basis for the prevention and control of microplastic pollution here. Full article

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13 pages, 1442 KB

Open AccessArticle

Degradation of Typical PPCPs During Anaerobic Digestion and in Soil

by Min Guo, Linyue Xu, Liguo Guo, Jie Hu and Ru Liu

Toxics 2025, 13(9), 780; https://doi.org/10.3390/toxics13090780 - 15 Sep 2025

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Abstract

Degradation is a key natural attenuation mechanism governing the fate of PPCPs during anaerobic digestion (AD) and subsequent soil exposure. Nevertheless, the combined impact of this sequential treatment (AD followed by land application) remains poorly understood. This study evaluated the degradation characteristics of [...] Read more.

Degradation is a key natural attenuation mechanism governing the fate of PPCPs during anaerobic digestion (AD) and subsequent soil exposure. Nevertheless, the combined impact of this sequential treatment (AD followed by land application) remains poorly understood. This study evaluated the degradation characteristics of nine PPCPs during mesophilic AD in three distinct soil types. The concentration changes in the nine PPCPs were monitored after 0, 5, 10, 15, 20, 25, and 30 days of anaerobic incubation at 38 °C, as well as after 0, 2, 5, 8, 10, 12, 15, 20, and 30 days of dark incubation at 25 °C with humidity at 75% in three soils. AD effectively removed sulfamethoxydiazine, ciprofloxacin, and oxytetracycline (>80%). The removal efficiencies for carbamazepine, progesterone, triclosan, naproxen, and megestrol acetate were relatively poor, with the removal rates ranging from 50% to 80%, while gemfibrozil exhibited minimal degradation (<50%). The degradation behavior of nine PPCPs fits well with first-order kinetic equations. Calculated half-lives (days) in the three soils were as follows: sulfamethoxydiazine (20.39 to 23.10), carbamazepine (36.48 to 77.02), megestrol acetate (11.18 to 20.39), progesterone (6.08 to 23.90), ciprofloxacin (11.75 to 63.01), oxytetracycline (13.08 to 30.14), naproxen (7.79 to 40.77), gemfibrozil (8.45 to 30.14), and triclosan (14.75 to 46.21). The corresponding R² values ranged from 0.8882 to 0.9320 for sulfamethoxydiazine, 0.8579 to 0.9248 for carbamazepine, 0.8745 to 0.9658 for megestrol acetate, 0.9026 to 0.9560 for progesterone, 0.8147 to 0.9571 for ciprofloxacin, 0.8136 to 0.9063 for oxytetracycline, 0.8961 to 0.9156 for naproxen, 0.8802 to 0.9497 for gemfibrozil, and 0.9099 to 0.9457 for triclosan. Soil physicochemical properties significantly influenced PPCP degradation rates. Gemfibrozil warrants immediate attention due to its poor degradation; the five PPCPs presenting moderate concern—namely carbamazepine, ciprofloxacin, oxytetracycline, naproxen, and triclosan—require further risk assessment, while sulfamethoxydiazine, megestrol acetate, and progesterone pose low persistence risk according to current evidence. Full article

(This article belongs to the Section Emerging Contaminants)

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19 pages, 10863 KB

Open AccessArticle

Effects of ZnFe₂O₄ Nanoparticles on Development and Rhythmic Behavior of Drosophila melanogaster

by Wenhao Yan, Yunfan Guo, Penghui Li, Ziyan Zhang, Jinjun Yang and Yongyan Sun

Toxics 2025, 13(9), 779; https://doi.org/10.3390/toxics13090779 - 14 Sep 2025

Viewed by 417

Abstract

Objectives: This study planned to determine the biological effects associated with ZnFe₂O₄-NPs exposure using Drosophila melanogaster as an in vivo model. Methods: ZnFe₂O₄-NPs were hydrothermally synthesized, and the development of offspring flies were [...] Read more.

Objectives: This study planned to determine the biological effects associated with ZnFe₂O₄-NPs exposure using Drosophila melanogaster as an in vivo model. Methods: ZnFe₂O₄-NPs were hydrothermally synthesized, and the development of offspring flies were evaluated via dietary exposure to different doses of ZnFe₂O₄-NPs (0, 200, 400, 600 μg/mL). Rhythmic behaviors of parent male flies were monitored. Results: Internalization of ZnFe₂O₄-NPs through the intestinal barrier occurred. Oral intake of ZnFe₂O₄-NPs decreased the eclosed adult numbers and perturbed the insect developmental process. In male flies, significant upregulation of HSPs and Turandot family genes was detected, accompanied by ROS reduction and suppressed antioxidant defense responses, and exposure of ZnFe₂O₄-NPs disrupted sleep patterns of males, including a reduction in sleep duration and aggravation of sleep fragmentation. Suppressed activity levels were also found after ZnFe₂O₄-NPs exposure. Significant increased expressions of circadian genes (Clk and Cyc) were detected, alongside elevation of neurotransmitter levels and related gene expressions. Conclusions: Overall, ZnFe₂O₄-NPs can perturb development process via inducing heat shock and detoxification response, and disrupted rhythmic behaviors may be attributed to elevation of neurotransmitter levels and upregulated gene expressions of circadian genes. Our findings may offer valuable insights for evaluating ecological risks of metal-based nanoparticles and suggest potential applications in developing novel pest management strategies by utilizing insect behavioral and physiological responses to nanomaterials. Full article

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15 pages, 809 KB

Open AccessArticle

Nationwide Monitoring and Risk Assessment of Pesticide Residues in Fishery Products

by Dong-ju Kim, Eun-been Oh, Jee-hyo Moon, Jeong-won Choi, Tae-hwa Kim, Seok-hee Lee, Ju-yeon Park, Chan-hyeok Kwon and Kee-sung Kyung

Toxics 2025, 13(9), 778; https://doi.org/10.3390/toxics13090778 - 14 Sep 2025

Viewed by 380

Abstract

Global production of fisheries and aquaculture products continues to increase, with the fisheries sector increasingly considered essential for global food security and nutrition. As public demand for seafood increases, implementation of safety management to minimize risks and ensure the safety of seafood products [...] Read more.

Global production of fisheries and aquaculture products continues to increase, with the fisheries sector increasingly considered essential for global food security and nutrition. As public demand for seafood increases, implementation of safety management to minimize risks and ensure the safety of seafood products becomes important. This study was conducted to monitor 198 chemicals, comprising 161 pesticides and 37 pesticide metabolites, and to assess their risks in saltwater and freshwater fish in the Republic of Korea, based on the analysis of 471 fishery samples (298 saltwater and 173 freshwater). Among the fifteen saltwater fish species analyzed, pesticides were detected in eight species (croaker, flatfish, sea bass, flounder, snapper, yellow tail, salmon, and gizzard shad) and in all seven freshwater species (carp, catfish, crucian carp, eel, leather carp, loach, and trout). Four pesticides (ethoxyquin, lufenuron, metaflumizone, and propiconazole) were detected in saltwater fish, while nine pesticides (ethoxyquin, ipfencarbazone, isoprothiolane, lufenuron, metaflumizone, oxadiargyl, pendimethalin, phoxim, and trichlorfon) were found in freshwater fish. Ethoxyquin was the most frequently detected pesticide in both fish types, mostly in the form of its metabolite, the ethoxyquin dimer. The estimated daily intake (EDI) was calculated based on the maximum concentrations of pesticides detected and the average consumption of fishery products by sex and age group. The hazard quotients, expressed as a percentage of acceptable daily intake (%ADI) and calculated using the EDI and ADI of the pesticides detected, were evaluated to be no more than 5.6%. These results suggest that consumption of saltwater and freshwater fish in the Republic of Korea poses a low risk to human health. This approach can be applied to pesticide residue monitoring and risk assessment in the fisheries sector, providing valuable data for evaluating contamination levels and supporting the regulation and management of chemical residues. Full article

(This article belongs to the Section Agrochemicals and Food Toxicology)

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24 pages, 10679 KB

Open AccessReview

Review of Health Hazards in High-Entropy Alloy Processing Under Laboratory Conditions and Risk Assessment Using a Simple Risk Scoring Model

by Sheetal Kumar Dewangan

Toxics 2025, 13(9), 777; https://doi.org/10.3390/toxics13090777 - 13 Sep 2025

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Abstract

Certain metal powders used in the synthesis and processing of high-entropy alloys (HEAs) pose significant health hazards, as many of these metals are toxic substances with no biological role in the human body. These metals can mimic essential elements or interfere with metabolic [...] Read more.

Certain metal powders used in the synthesis and processing of high-entropy alloys (HEAs) pose significant health hazards, as many of these metals are toxic substances with no biological role in the human body. These metals can mimic essential elements or interfere with metabolic processes (the chemical reactions in living organisms that sustain life), leading to detrimental health effects. While some metals, such as aluminum, can be eliminated from the body through natural biological processes, others tend to accumulate, causing chronic illnesses over time. This review examines the toxicity mechanisms and health impacts of metals used in HEA synthesis, focusing on laboratory-scale processing. It also identifies potential health risks associated with occupational exposure in laboratory environments, including the inhalation of toxic metal powders and nanoparticles. A simple risk scoring model is introduced to systematically assess and quantify these risks based on factors such as toxicity levels, exposure limits, and carcinogenic potential (the ability of a substance to cause cancer) as given by the Occupational Safety and Health Administration (OSHA) and the National Institute for Occupational Safety and Health (NIOSH) standards. The proposed model can be applied to evaluate the relative hazards of commonly used HEA constituent powders (e.g., Ni, Co, Cr, and Al), offering practical guidance for safer laboratory handling and material selection. By integrating this risk assessment framework, this review aims to enhance workplace safety, guide the development of better material handling practices, and assist researchers in mitigating health risks associated with HEA processing. Full article

(This article belongs to the Special Issue Health Risk Assessment of Exposure to Emerging Contaminants)

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Toxics, Volume 13, Issue 9 (September 2025) – 101 articles

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