Overexpression of Douglas-Fir LEAFY COTYLEDON1 (PmLEC1) in Arabidopsis Induces Embryonic Programs and Embryo-like Structures in the lec1-1 Mutant but Not in Wild Type Plants
Abstract
:1. Introduction
2. Results
2.1. PmLEC1 Rescues the lec1-1 Null Mutant, Inhibits Vegetative Development and Leads to the Formation of Embryo-like Structures in Abnormal T1 Seedlings
2.2. Ectopic Expression of PmLEC1 in the lec1-1PmLEC1 T2 Generation Induces Embryonic Programs and Spontaneous Formation of Embryo-like Structures
2.3. Overexpression of PmLEC1 in Wild Type Plants Reduces Apical Dominance but Does Not Activate Embryonic Programs
2.4. PmLEC1 Protein Accumulation in Transgenic Plants Correlates with Embryo-like Character and Suppression of Vegetative Development
3. Discussion
3.1. Douglas-Fir PmLEC1 Is Functionally Homologous to Arabidopsis LEC1 and Induces Embryonic Programs in the Arabidopsis lec1-1 Null Mutant
3.2. Transgene Silencing May Account for the Episodes of Normal Development Observed in lec1-1PmLEC1 T1 and T2 Transgenic Plants
3.3. PmLEC1 May Function Prior to Embryogenesis
3.4. LEC1 by Itself Cannot Induce De Novo Embryogenesis
3.5. The AtLEC1 Promoter Region May Exert Control over Embryogenesis
4. Materials and Methods
4.1. Plant Material
4.2. Construction of Expression Cassettes
4.3. Agrobacterium-Mediated Plant Transformation
4.4. DNA Isolation and PCR Analysis
4.5. RNA Isolation and RT-PCR Analysis
4.6. Western Blot Analysis
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Vetrici, M.A.; Yevtushenko, D.P.; Misra, S. Overexpression of Douglas-Fir LEAFY COTYLEDON1 (PmLEC1) in Arabidopsis Induces Embryonic Programs and Embryo-like Structures in the lec1-1 Mutant but Not in Wild Type Plants. Plants 2021, 10, 1526. https://doi.org/10.3390/plants10081526
Vetrici MA, Yevtushenko DP, Misra S. Overexpression of Douglas-Fir LEAFY COTYLEDON1 (PmLEC1) in Arabidopsis Induces Embryonic Programs and Embryo-like Structures in the lec1-1 Mutant but Not in Wild Type Plants. Plants. 2021; 10(8):1526. https://doi.org/10.3390/plants10081526
Chicago/Turabian StyleVetrici, Mariana A., Dmytro P. Yevtushenko, and Santosh Misra. 2021. "Overexpression of Douglas-Fir LEAFY COTYLEDON1 (PmLEC1) in Arabidopsis Induces Embryonic Programs and Embryo-like Structures in the lec1-1 Mutant but Not in Wild Type Plants" Plants 10, no. 8: 1526. https://doi.org/10.3390/plants10081526