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Alterations in Synthesis and Repair of DNA during the Development of Loach Misgurnus fossilis

Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov Square, 123182 Moscow, Russia
Author to whom correspondence should be addressed.
Academic Editor: Andy Wessels
J. Dev. Biol. 2016, 4(1), 6;
Received: 16 December 2015 / Revised: 18 January 2016 / Accepted: 20 January 2016 / Published: 27 January 2016
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Using a modified radiolabeled primer extension method (we named this modification misGvA—“misincorporation of G versus A”) we have investigated the DNA synthesis and repair at early and late stages of development of loach Misgurnus fossilis. The misincorporation activity of DNA polymerase iota (Pol ι) in wild-type loach could not be detected by this method at any stage of loach development. In transgenic loach overexpressing human Pol ι we have shown that the bypassing of DNA synthesis arrest after incorporation of mismatched nucleotide by Pol ι (the T-stop) was not associated with this enzyme. Non-transgenic loach larvae are virtually lacking the capacity for error correction of DNA duplex containing a mismatched nucleotide. Such repair activity develops only in the adult fish. It appears that the initial stages of development are characterized by more intensive DNA synthesis, while in terminal stages the repair activities become more prominent. The misGvA approach clearly indicates substantial changes in the DNA synthesis intensity, although the role of particular replicative and repair DNA polymerases in this process requires further study. View Full-Text
Keywords: DNA polymerase iota; loach; DNA synthesis; DNA repair DNA polymerase iota; loach; DNA synthesis; DNA repair

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Gening, L.V.; Lakhin, A.V.; Makarova, I.V.; Nenasheva, V.V.; Andreeva, L.E.; Tarantul, V.Z. Alterations in Synthesis and Repair of DNA during the Development of Loach Misgurnus fossilis. J. Dev. Biol. 2016, 4, 6.

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