Next Article in Journal
A Bioorthogonally Applicable, Fluorogenic, Large Stokes-Shift Probe for Intracellular Super-Resolution Imaging of Proteins
Next Article in Special Issue
Variations in Circulating Active MMP-9 Levels during Renal Replacement Therapy
Previous Article in Journal
Hypothalamic NAD+-Sirtuin Axis: Function and Regulation
Previous Article in Special Issue
Expression of MMP-2, MMP-9, and NGAL in Tissue and Serum of Patients with Vascular Aneurysms and Their Modulation by Statin Treatment: A Pilot Study
Article

An Oral Rinse Active Matrix Metalloproteinase-8 Point-of-Care Immunotest May Be Less Accurate in Patients with Crohn’s Disease

1
Department of Oral Pathology and Oral Radiology, Institute of Dentistry, University of Turku, 20520 Turku, Finland
2
Department of Pathology, Turku University Hospital, 20521 Turku, Finland
3
Department of Periodontology, Institute of Dentistry, University of Turku, 20520 Turku, Finland
4
Oral Health Care, Welfare Division, City of Turku, 20101 Turku, Finland
5
Department of Oral and Maxillofacial Disease, Helsinki University Hospital, University of Helsinki, 00014 Helsinki, Finland
6
Department of Dental Medicine, Karolinska Institute, 14152 Huddinge, Sweden
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(3), 395; https://doi.org/10.3390/biom10030395
Received: 3 February 2020 / Accepted: 26 February 2020 / Published: 4 March 2020
(This article belongs to the Special Issue Matrix Metalloproteinases in Health and Disease)
The diagnostic accuracy of point-of-care (PoC) applications may be compromised in individuals with additional inflammatory conditions. This cross-sectional study examined the performance of a commercial oral rinse active matrix metalloproteinase-8 (aMMP-8) PoC immunotest in individuals with (n = 47) and without Crohn’s disease (CD) (n = 41). Oral rinse collected from the participants was analyzed by the PoC immunotest. Molecular forms and fragments of salivary MMP-8 were detected by western immunoblotting. The sensitivity of the immunotest for periodontitis was 60.0% in the CD group and 90.0% in the control group. The respective specificity was 75.0% and 80.0%. In both groups, clinical diagnosis of periodontitis exhibited a significant association with the immunotest results, however, the odds ratio (OR) was more than ten-fold in controls (OR 54.3, 95% CI: 3.1–953, p = 0.006) in comparison to CD patients (OR 5.2, 95% CI: 1.3–21.6, p = 0.022). According to Western immunoblot results, the immunotest MMP-8 positivity was not related to elevated levels of molecular forms and fragments of MMP-8 in the CD group, as in the control group. The diagnostic accuracy of the aMMP-8 PoC oral rinse immunotest is reduced in CD patients, which may be related to lower levels or undetectable complexes. View Full-Text
Keywords: collagenases; Crohn’s disease; dental caries; mouth; periodontitis collagenases; Crohn’s disease; dental caries; mouth; periodontitis
Show Figures

Figure 1

MDPI and ACS Style

Rautava, J.; Gürsoy, U.K.; Kullström, A.; Könönen, E.; Sorsa, T.; Tervahartiala, T.; Gürsoy, M. An Oral Rinse Active Matrix Metalloproteinase-8 Point-of-Care Immunotest May Be Less Accurate in Patients with Crohn’s Disease. Biomolecules 2020, 10, 395. https://doi.org/10.3390/biom10030395

AMA Style

Rautava J, Gürsoy UK, Kullström A, Könönen E, Sorsa T, Tervahartiala T, Gürsoy M. An Oral Rinse Active Matrix Metalloproteinase-8 Point-of-Care Immunotest May Be Less Accurate in Patients with Crohn’s Disease. Biomolecules. 2020; 10(3):395. https://doi.org/10.3390/biom10030395

Chicago/Turabian Style

Rautava, Jaana, Ulvi K. Gürsoy, Adrian Kullström, Eija Könönen, Timo Sorsa, Taina Tervahartiala, and Mervi Gürsoy. 2020. "An Oral Rinse Active Matrix Metalloproteinase-8 Point-of-Care Immunotest May Be Less Accurate in Patients with Crohn’s Disease" Biomolecules 10, no. 3: 395. https://doi.org/10.3390/biom10030395

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop