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Article

Zn-Doped CaP-Based Coatings on Ti–6Al–4V and Ti–6Al–7Nb Alloys Prepared by Magnetron Sputtering: Controllable Biodegradation, Bacteriostatic, and Osteogenic Activities

1
Laboratory of Physics of Nanostructured Biocomposites, Institute of Strength Physics and Materials Science SB RAS (ISPMS SB RAS), 634055 Tomsk, Russia
2
LLC “SPC SINTEL”, 634061 Tomsk, Russia
3
Center for Immunology and Cell Biotechnology, Immanuel Kant Baltic Federal University, 236029 Kaliningrad, Russia
4
Siberian Federal Scientific and Clinical Center of the Federal Medical-Biological Agency, 636070 Seversk, Russia
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Division of Traumatology and Orthopedics, Regional Clinical Emergency Hospital, 650000 Kemerovo, Russia
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Medical Officer for Science, Regional Clinical Emergency Hospital, 650000 Kemerovo, Russia
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Department of General Oncology, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, 634009 Tomsk, Russia
8
Research School of High-Energy Physics, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia
9
National Institute of Research and Development for Optoelectronics INOE2000, Magurele, 077125 Bucharest, Romania
10
Research School of Chemistry and Applied Biomedical Sciences, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia
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Department of Morphology and General Pathology, Siberian State Medical University, 634050 Tomsk, Russia
*
Authors to whom correspondence should be addressed.
Academic Editors: Seunghan Oh and Elvira De Giglio
Coatings 2021, 11(7), 809; https://doi.org/10.3390/coatings11070809
Received: 12 April 2021 / Revised: 24 June 2021 / Accepted: 29 June 2021 / Published: 3 July 2021
(This article belongs to the Special Issue Surface Modification of Medical Implants)
New TiNb-based alloys, such as Ti–6Al–7Nb, are currently being studied around the world as an alternative to other Ti alloys, e.g., instead of Ti–6Al–4V. We conducted a pilot study where thin (approximately 1.2 micron) CaP coatings containing low doses of Zn2+ (0.4–0.8 wt.%) were prepared by the radio frequency magnetron sputtering (RFMS) of Zn-hydroxyapatite (HA) target on Ti–6Al–4V and Ti–6Al–7Nb substrates and investigated their physicochemical properties, in vitro solubility, cytotoxicity, and antibacterial and osteogenic activities. The thickness of the obtained coatings was approximately 1.2–1.3 microns. Zn substitution did not result in roughness or structural or surface changes in the amorphous CaP coatings. The distributions of Ca, P, and Zn were homogeneous across the film thickness as shown by the EDX mapping of these elements. Zn doping of CaP coatings on both types of Ti-based alloys statistically influenced the results of the scratch-test. However, obtained values are satisfactory to use Zn-CaP coatings on biomedical implants. Increased Zn2+ release vs. tapered output of Ca and phosphate ions occurred during 5 weeks of an in vitro immersion test in 0.9% NaCl solution. Ti–6Al–7Nb alloy, unlike Ti–6Al–4V, promoted more linear biodegradation of CaP coatings in vitro. As a result, CaP-based surfaces on Ti–6Al–7Nb, compared with on Ti–6Al–4V alloy, augmented the total areas of Alizarin red staining in a 21-day culture of human adipose-derived mesenchymal stem cells in a statistically significant manner. Moreover, Zn–CaP coatings statistically reduced leukemic Jurkat T cell survival within 48 h of in vitro culture. Along with the higher solubility of the Zn–CaP surface, a greater reduction (4- to 5.5-fold) in Staphylococcus aureus growth was observed in vitro when 7-day extracts of the coatings were added into the microbial culture. Hence, Zn–CaP-coated Ti–6Al–7Nb alloy with controllable biodegradation as prepared by RFMS is a prospective material suitable for bone applications in cases where there is a risk of bacterial contamination with severe consequences, for example, in leukemic patients. Further research is needed to closely investigate the mechanical features and pathways of their solubility and antimicrobial, antitumor, and osteogenic activities. View Full-Text
Keywords: element mapping; thickness; hardness; roughness; in vitro solubility; cytotoxicity; leukemic cells; human adipose-derived mesenchymal stem cells; Alizarin red staining; Staphylococcus aureus growth element mapping; thickness; hardness; roughness; in vitro solubility; cytotoxicity; leukemic cells; human adipose-derived mesenchymal stem cells; Alizarin red staining; Staphylococcus aureus growth
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MDPI and ACS Style

Prosolov, K.A.; Mitrichenko, D.V.; Prosolov, A.B.; Nikolaeva, O.O.; Lastovka, V.V.; Belyavskaya, O.A.; Chebodaeva, V.A.; Glukhov, I.A.; Litvinova, L.S.; Shupletsova, V.V.; Khaziakhmatova, O.G.; Malashchenko, V.V.; Yurova, K.A.; Shunkin, E.O.; Fedorov, M.A.; Komkov, A.R.; Pavlenko, V.V.; Anisenya, I.I.; Sharkeev, Y.P.; Vladescu, A.; Khlusov, I.A. Zn-Doped CaP-Based Coatings on Ti–6Al–4V and Ti–6Al–7Nb Alloys Prepared by Magnetron Sputtering: Controllable Biodegradation, Bacteriostatic, and Osteogenic Activities. Coatings 2021, 11, 809. https://doi.org/10.3390/coatings11070809

AMA Style

Prosolov KA, Mitrichenko DV, Prosolov AB, Nikolaeva OO, Lastovka VV, Belyavskaya OA, Chebodaeva VA, Glukhov IA, Litvinova LS, Shupletsova VV, Khaziakhmatova OG, Malashchenko VV, Yurova KA, Shunkin EO, Fedorov MA, Komkov AR, Pavlenko VV, Anisenya II, Sharkeev YP, Vladescu A, Khlusov IA. Zn-Doped CaP-Based Coatings on Ti–6Al–4V and Ti–6Al–7Nb Alloys Prepared by Magnetron Sputtering: Controllable Biodegradation, Bacteriostatic, and Osteogenic Activities. Coatings. 2021; 11(7):809. https://doi.org/10.3390/coatings11070809

Chicago/Turabian Style

Prosolov, Konstantin A., Dmitrii V. Mitrichenko, Aleksandr B. Prosolov, Olga O. Nikolaeva, Vladimir V. Lastovka, Olga A. Belyavskaya, Valentina A. Chebodaeva, Ivan A. Glukhov, Larisa S. Litvinova, Valeria V. Shupletsova, Olga G. Khaziakhmatova, Vladimir V. Malashchenko, Kristina A. Yurova, Egor O. Shunkin, Maxim A. Fedorov, Andrei R. Komkov, Vladimir V. Pavlenko, Ilya I. Anisenya, Yurii P. Sharkeev, Alina Vladescu, and Igor A. Khlusov 2021. "Zn-Doped CaP-Based Coatings on Ti–6Al–4V and Ti–6Al–7Nb Alloys Prepared by Magnetron Sputtering: Controllable Biodegradation, Bacteriostatic, and Osteogenic Activities" Coatings 11, no. 7: 809. https://doi.org/10.3390/coatings11070809

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