Degradable Dextran Nanopolymer as a Carrier for Choline Kinase (ChoK) siRNA Cancer Therapy
JHU ICMIC Program, Division of Cancer Imaging Research, Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
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Academic Editor: Thomas Nann
Nanomaterials 2016, 6(2), 34; https://doi.org/10.3390/nano6020034
Received: 27 October 2015 / Revised: 19 January 2016 / Accepted: 4 February 2016 / Published: 22 February 2016
(This article belongs to the Special Issue Nanomaterials for Cancer Therapies)
Although small interfering RNA (siRNA) therapy has proven to be a specific and effective treatment in cells, the delivery of siRNA is a challenge for the applications of siRNA therapy. We present a degradable dextran with amine groups as an siRNA nano-carrier. In our nano-carrier, the amine groups are conjugated to the dextran platform through the acetal bonds, which are acid sensitive. Therefore this siRNA carrier is stable in neutral and basic conditions, while the amine groups can be cleaved and released from dextran platform under weak acid conditions (such as in endosomes). The cleavage and release of amine groups can reduce the toxicity of cationic polymer and enhance the transfection efficiency. We successfully applied this nano-carrier to deliver choline kinase (ChoK) siRNA for ChoK inhibition in cells.
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Keywords:
siRNA therapy; choline kinase; dextran; nano-polymer; siRNA delivery
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
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MDPI and ACS Style
Chen, Z.; Krishnamachary, B.; Bhujwalla, Z.M. Degradable Dextran Nanopolymer as a Carrier for Choline Kinase (ChoK) siRNA Cancer Therapy. Nanomaterials 2016, 6, 34. https://doi.org/10.3390/nano6020034
AMA Style
Chen Z, Krishnamachary B, Bhujwalla ZM. Degradable Dextran Nanopolymer as a Carrier for Choline Kinase (ChoK) siRNA Cancer Therapy. Nanomaterials. 2016; 6(2):34. https://doi.org/10.3390/nano6020034
Chicago/Turabian StyleChen, Zhihang; Krishnamachary, Balaji; Bhujwalla, Zaver M. 2016. "Degradable Dextran Nanopolymer as a Carrier for Choline Kinase (ChoK) siRNA Cancer Therapy" Nanomaterials 6, no. 2: 34. https://doi.org/10.3390/nano6020034
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