Antimicrobial Activity of Nitric Oxide-Releasing Ti-6Al-4V Metal Oxide
AbstractTitanium and titanium alloy materials are commonly used in joint replacements, due to the high strength of the materials. Pathogenic microorganisms can easily adhere to the surface of the metal implant, leading to an increased potential for implant failure. The surface of a titanium-aluminum-vanadium (Ti-6Al-4V) metal oxide implant material was functionalized to deliver an small antibacterial molecule, nitric oxide. S-nitroso-penicillamine, a S-nitrosothiol nitric oxide donor, was covalently immobilized on the metal oxide surface using self-assembled monolayers. Infrared spectroscopy was used to confirm the attachment of the S-nitrosothiol donor to the Ti-Al-4V surface. Attachment of S-nitroso-penicillamine resulted in a nitric oxide (NO) release of 89.6 ± 4.8 nmol/cm2 under physiological conditions. This low concentration of nitric oxide reduced Escherichia coli and Staphylococcus epidermidis growth by 41.5 ± 1.2% and 25.3 ± 0.6%, respectively. Combining the S-nitrosothiol releasing Ti-6Al-4V with tetracycline, a commonly-prescribed antibiotic, increased the effectiveness of the antibiotic by 35.4 ± 1.3%, which allows for lower doses of antibiotics to be used. A synergistic effect of ampicillin with S-nitroso-penicillamine-modified Ti-6Al-4V against S. epidermidis was not observed. The functionalized Ti-6Al-4V surface was not cytotoxic to mouse fibroblasts. View Full-Text
Share & Cite This Article
Reger, N.A.; Meng, W.S.; Gawalt, E.S. Antimicrobial Activity of Nitric Oxide-Releasing Ti-6Al-4V Metal Oxide. J. Funct. Biomater. 2017, 8, 20.
Reger NA, Meng WS, Gawalt ES. Antimicrobial Activity of Nitric Oxide-Releasing Ti-6Al-4V Metal Oxide. Journal of Functional Biomaterials. 2017; 8(2):20.Chicago/Turabian Style
Reger, Nina A.; Meng, Wilson S.; Gawalt, Ellen S. 2017. "Antimicrobial Activity of Nitric Oxide-Releasing Ti-6Al-4V Metal Oxide." J. Funct. Biomater. 8, no. 2: 20.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.