Next Article in Journal
Fibrosis Distinguishes Critical Limb Ischemia Patients from Claudicants in a Transcriptomic and Histologic Analysis
Previous Article in Journal
Short-Term Results of Ocriplasmin versus Prompt Vitrectomy for Macular Hole. Which Performs Better?
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
JCM
Volume 9
Issue 12
10.3390/jcm9123973
jcm-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Chronic Rhinosinusitis: MALDI-TOF Mass Spectrometry Microbiological Diagnosis and Electron Microscopy Analysis; Experience of the 2nd Otorhinolaryngology Clinic of Cluj-Napoca, Romania

by
Ionuț Isaia Jeican
1,2,
Lucian Barbu Tudoran
3,4,
Adrian Florea
5,
Mirela Flonta
6,
Veronica Trombitas
1,
Anda Apostol
1,
Mihai Dumitru
7,8,
Maria Aluaș
9,*,
Lia Monica Junie
10 and
Silviu Albu
1
1
Department of Head and Neck Surgery and Otorhinolaryngology, University Clinical Hospital of Railway Company, Iuliu Hatieganu University of Medicine and Pharmacy, 16-20 Republicii, 400015 Cluj-Napoca, Romania
2
Department of Anatomy and Embryology, Iuliu Hatieganu University of Medicine and Pharmacy, 3-5 Clinicilor, 400006 Cluj-Napoca, Romania
3
Electron Microscopy Laboratory Prof. C. Craciun, Faculty of Biology and Geology, Babes-Bolyai University, 5-7 Clinicilor, 400006 Cluj-Napoca, Romania
4
Electron Microscopy Integrated Laboratory, National Institute for R&D of Isotopic and Molecular Technologies, 67-103 Donath, 400293 Cluj-Napoca, Romania
5
Department of Cell and Molecular Biology, Iuliu Hatieganu University of Medicine and Pharmacy, 6 Louis Pasteur, 400349 Cluj-Napoca, Romania
6
Infectious Diseases Hospital of Cluj-Napoca, 23 Iuliu Moldovan, 400348 Cluj-Napoca, Romania
7
Department of Head and Neck Surgery and Otorhinolaryngology, Bucharest University Emergency Hospital, 169 Splaiul Independenței, 010271 Bucharest, Romania
8
Department of Anatomy and Embryology, Carol Davila University of Medicine and Pharmacy, 1 Frosa Sarandy, 050474 Bucharest, Romania
9
Department of Oral Health, Iuliu Hatieganu University of Medicine and Pharmacy, 15 Victor Babes, 400012 Cluj-Napoca, Romania
10
Department of Microbiology, Iuliu Hatieganu University of Medicine and Pharmacy, 6 Louis Pasteur Street, 400349 Cluj-Napoca, Romania
*
Author to whom correspondence should be addressed.
J. Clin. Med. 2020, 9(12), 3973; https://doi.org/10.3390/jcm9123973
Submission received: 3 November 2020 / Revised: 27 November 2020 / Accepted: 2 December 2020 / Published: 8 December 2020
(This article belongs to the Section Otolaryngology)
Browse Figures
Versions Notes

Abstract

(1) Background: Chronic rhinosinusitis (CRS) represents a wide range of infectious-inflammatory processes affecting, simultaneously, the nose and paranasal sinuses mucosa. The paper presents outcomes of the investigation of CRS microbiological characteristics in a group of 32 patients. (2) Methods: The purulent samples were collected during functional endoscopic sinus surgery. Agar plates were incubated and examined. All types of colonies were identified using Matrix-Assisted Laser Desorption - Ionisation-Time of Flight Mass Spectrometry (MALDI-TOF MS). For scanning electron microscopy, samples were fixed and sputter-coated with 10 nm gold and analyzed using a scanning electron microscope. For transmission electron microscopy, samples were fixed, postfixed, and dehydrated. After polymerization, ultrathin sections were collected on carbon coated copper grids and analyzed with Jeol JEM1010 TEM. (3) Results: Positive microbiological diagnosis was obtained in 62.5% of cases. The most frequent species found are Staphylococcus aureus and Streptococcus constellatus subsp. pharyngis. Corynebacterium aurimucosum and Eggerthia catenaformis were unreported species in CRS until the present. Biofilm was evidenced in 43.7% of sinus mucosa samples. Ciliary disorientation, atrophy, and no ciliated cells were also identified. (4) Conclusion: The microbial factor—pathogen or opportunistic—is one of the most important pathological links in chronic rhinosinusitis. MALDI-TOF MS allows easily and quickly identification of germs.
Keywords: chronic rhinosinusitis (CRS); odontogenic sinusitis; biofilms; MALDI-TOF MS; endoscopic sinus surgery chronic rhinosinusitis (CRS); odontogenic sinusitis; biofilms; MALDI-TOF MS; endoscopic sinus surgery

Share and Cite

MDPI and ACS Style

Jeican, I.I.; Barbu Tudoran, L.; Florea, A.; Flonta, M.; Trombitas, V.; Apostol, A.; Dumitru, M.; Aluaș, M.; Junie, L.M.; Albu, S. Chronic Rhinosinusitis: MALDI-TOF Mass Spectrometry Microbiological Diagnosis and Electron Microscopy Analysis; Experience of the 2nd Otorhinolaryngology Clinic of Cluj-Napoca, Romania. J. Clin. Med. 2020, 9, 3973. https://doi.org/10.3390/jcm9123973

AMA Style

Jeican II, Barbu Tudoran L, Florea A, Flonta M, Trombitas V, Apostol A, Dumitru M, Aluaș M, Junie LM, Albu S. Chronic Rhinosinusitis: MALDI-TOF Mass Spectrometry Microbiological Diagnosis and Electron Microscopy Analysis; Experience of the 2nd Otorhinolaryngology Clinic of Cluj-Napoca, Romania. Journal of Clinical Medicine. 2020; 9(12):3973. https://doi.org/10.3390/jcm9123973

Chicago/Turabian Style

Jeican, Ionuț Isaia, Lucian Barbu Tudoran, Adrian Florea, Mirela Flonta, Veronica Trombitas, Anda Apostol, Mihai Dumitru, Maria Aluaș, Lia Monica Junie, and Silviu Albu. 2020. "Chronic Rhinosinusitis: MALDI-TOF Mass Spectrometry Microbiological Diagnosis and Electron Microscopy Analysis; Experience of the 2nd Otorhinolaryngology Clinic of Cluj-Napoca, Romania" Journal of Clinical Medicine 9, no. 12: 3973. https://doi.org/10.3390/jcm9123973

APA Style

Jeican, I. I., Barbu Tudoran, L., Florea, A., Flonta, M., Trombitas, V., Apostol, A., Dumitru, M., Aluaș, M., Junie, L. M., & Albu, S. (2020). Chronic Rhinosinusitis: MALDI-TOF Mass Spectrometry Microbiological Diagnosis and Electron Microscopy Analysis; Experience of the 2nd Otorhinolaryngology Clinic of Cluj-Napoca, Romania. Journal of Clinical Medicine, 9(12), 3973. https://doi.org/10.3390/jcm9123973

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop