Next Article in Journal
Inhibitory Effect and Mechanism of Action of Quercetin and Quercetin Diels-Alder anti-Dimer on Erastin-Induced Ferroptosis in Bone Marrow-Derived Mesenchymal Stem Cells
Next Article in Special Issue
Diabetes Upregulates Oxidative Stress and Downregulates Cardiac Protection to Exacerbate Myocardial Ischemia/Reperfusion Injury in Rats
Previous Article in Journal
Influence of Postharvest Temperatures on Carotenoid Biosynthesis and Phytochemicals in Mature Green Chili (Capsicum annuum L.)
Previous Article in Special Issue
Exposure to Toxic Heavy Metals Can Influence Homocysteine Metabolism?
Article

MiR155-5p Inhibits Cell Migration and Oxidative Stress in Vascular Smooth Muscle Cells of Spontaneously Hypertensive Rats

1
Key Laboratory of Targeted Intervention of Cardiovascular Disease, Collaborative Innovation Center of Translational Medicine for Cardiovascular Disease, and Department of Physiology, Nanjing Medical University, Nanjing 211166, China
2
Department of Pathophysiology, Nanjing Medical University, Nanjing 211166, China
3
Department of Physiology and Pathophysiology, Cardiovascular Research Center, Xi’an Jiaotong University School of Medicine, Xi’an 710061, China
*
Author to whom correspondence should be addressed.
Antioxidants 2020, 9(3), 204; https://doi.org/10.3390/antiox9030204
Received: 1 February 2020 / Revised: 23 February 2020 / Accepted: 26 February 2020 / Published: 1 March 2020
(This article belongs to the Special Issue Free Radicals and Cardiovascular Diseases)
Migration of vascular smooth muscle cells (VSMCs) is essential for vascular reconstruction in hypertension and several vascular diseases. Our recent study showed that extracellular vesicles derived from vascular adventitial fibroblasts of normal rats inhibited VSMC proliferation by delivering miR155-5p to VSMCs. It is unknown whether miR155-5p inhibits cell migration and oxidative stress in VSMCs of spontaneously hypertensive rats (SHR) and in angiotensin II (Ang II)-treated VSMCs. The purpose of this study was to determine the role of miR155-5p in VSMC migration and its underlying mechanisms. Primary VSMCs were isolated from the aortic media of Wistar-Kyoto rats (WKY) and SHR. Wound healing assay and Boyden chamber assay were used to evaluate VSMC migration. A miR155-5p mimic inhibited, and a miR155-5p inhibitor promoted the migration of VSMC of SHR but had no significant effect on the migration of VSMC of WKY. The miR155-5p mimic inhibited angiotensin-converting enzyme (ACE) mRNA and protein expression in VSMCs. It also reduced superoxide anion production, NAD(P)H oxidase (NOX) activity, as well as NOX2, interleukin-1β (IL-1β), and tumor necrosis factor α (TNF-α) expression levels in VSMCs of SHR but not in VSMCs of WKY rats. Overexpression of miR155-5p inhibited VSMC migration and superoxide anion and IL-1β production in VSMCs of SHR but had no impact on exogenous Ang II-induced VSMC migration and on superoxide anion and IL-1β production in WKY rats and SHR. These results indicate that miR155-5p inhibits VSMC migration in SHR by suppressing ACE expression and its downstream production of Ang II, superoxide anion, and inflammatory factors. However, miR155-5p had no effects on exogenous Ang II-induced VSMC migration. View Full-Text
Keywords: microRNA; vascular smooth muscle cells; hypertension; oxidative stress; inflammation microRNA; vascular smooth muscle cells; hypertension; oxidative stress; inflammation
Show Figures

Figure 1

MDPI and ACS Style

Wu, N.; Ye, C.; Zheng, F.; Wan, G.-W.; Wu, L.-L.; Chen, Q.; Li, Y.-H.; Kang, Y.-M.; Zhu, G.-Q. MiR155-5p Inhibits Cell Migration and Oxidative Stress in Vascular Smooth Muscle Cells of Spontaneously Hypertensive Rats. Antioxidants 2020, 9, 204. https://doi.org/10.3390/antiox9030204

AMA Style

Wu N, Ye C, Zheng F, Wan G-W, Wu L-L, Chen Q, Li Y-H, Kang Y-M, Zhu G-Q. MiR155-5p Inhibits Cell Migration and Oxidative Stress in Vascular Smooth Muscle Cells of Spontaneously Hypertensive Rats. Antioxidants. 2020; 9(3):204. https://doi.org/10.3390/antiox9030204

Chicago/Turabian Style

Wu, Nan, Chao Ye, Fen Zheng, Guo-Wei Wan, Lu-Lu Wu, Qi Chen, Yue-Hua Li, Yu-Ming Kang, and Guo-Qing Zhu. 2020. "MiR155-5p Inhibits Cell Migration and Oxidative Stress in Vascular Smooth Muscle Cells of Spontaneously Hypertensive Rats" Antioxidants 9, no. 3: 204. https://doi.org/10.3390/antiox9030204

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop