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Article

Transcriptomic Analysis Reveals Cu/Zn SODs Acting as Hub Genes of SODs in Hylocereus undatus Induced by Trypsin during Storage

by 1,†, 2,†, 2, 2, 2 and 2,3,4,*
1
College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang 471023, China
2
College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China
3
Henan Engineering Research Center of Food Microbiology, Luoyang 471000, China
4
National Demonstration Center for Experimental Food Processing and Safety Education, Luoyang 471000, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Antioxidants 2020, 9(2), 162; https://doi.org/10.3390/antiox9020162
Received: 2 January 2020 / Revised: 14 February 2020 / Accepted: 15 February 2020 / Published: 17 February 2020
It has been revealed by us that superoxide scavenging is a new activity of trypsin. In this study, the synergistic mechanisms of trypsin and superoxide dismutases (SODs) were evaluated in Hylocereus undatus (pitaya). Trypsin significantly improved the storage quality of H. undatus, including weight loss impediment and decrease of cellular injury. The regulatory mechanisms of 16 SOD genes by trypsin were revealed using transcriptomic analysis on H. undatus. Results revealed that important physiological metabolisms, such as antioxidant activities or metal ion transport were induced, and defense responses were inhibited by trypsin. Furthermore, the results of protein–protein interaction (PPI) networks showed that besides the entire ROS network, the tiny SODs sub-network was also a scale-free network. Cu/Zn SODs acted as the hub that SODs synergized with trypsin during the storage of H. undatus. View Full-Text
Keywords: Hylocereus undatus (H. undatus); cytoscape; protein-protein interaction (PPI); superoxide dismutase (SOD); trypsin Hylocereus undatus (H. undatus); cytoscape; protein-protein interaction (PPI); superoxide dismutase (SOD); trypsin
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MDPI and ACS Style

Pang, X.; Li, X.; Liu, X.; Cai, L.; Li, B.; Li, X. Transcriptomic Analysis Reveals Cu/Zn SODs Acting as Hub Genes of SODs in Hylocereus undatus Induced by Trypsin during Storage. Antioxidants 2020, 9, 162. https://doi.org/10.3390/antiox9020162

AMA Style

Pang X, Li X, Liu X, Cai L, Li B, Li X. Transcriptomic Analysis Reveals Cu/Zn SODs Acting as Hub Genes of SODs in Hylocereus undatus Induced by Trypsin during Storage. Antioxidants. 2020; 9(2):162. https://doi.org/10.3390/antiox9020162

Chicago/Turabian Style

Pang, Xinyue, Xinling Li, Xueru Liu, Luning Cai, Bairu Li, and Xin Li. 2020. "Transcriptomic Analysis Reveals Cu/Zn SODs Acting as Hub Genes of SODs in Hylocereus undatus Induced by Trypsin during Storage" Antioxidants 9, no. 2: 162. https://doi.org/10.3390/antiox9020162

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