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Applied SciencesApplied Sciences
  • Review
  • Open Access

25 May 2024

33 Pages

Application of Natural Edible Coating to Enhance the Shelf Life of Red Fruits and Their Bioactive Content

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and
1
Department of Pharmacy and Health and Nutritional Sciences, University of Calabria, 87036 Arcavacata di Rende, Italy
2
Department of Food and Human Nutrition, University of Manitoba, Winnipeg, MB R3T 2N2, Canada
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Authors to whom correspondence should be addressed.
This article belongs to the Special Issue Antioxidant Compounds in Food Processing

Abstract

Red fruits contain bioactive substances including phenolic acids and flavonoids, which provide many health advantages for the human body. Industries find them intriguing because of their color and their ability to prevent chronic ailments such as metabolic, degenerative, and cardiovascular disorders. Nevertheless, the resilience of these organic molecules is influenced by several environmental, physical, and chemical phenomena. Therefore, the beneficial health properties of red fruits may diminish during postharvest processing. In this scenario, many postharvest methods have been implemented to enhance the shelf life and preserve the bioactive components of red fruits. The objectives of this review were to provide a comprehensive assessment of the health benefits of red fruits, and to explore the possibilities of edible coatings in retaining their freshness and protecting their bioactive contents. Co-occurrence networks were built using VOSviewer software to produce a two-dimensional map based on term frequency, and the examination of the 1364 keywords obtained from the scientific papers revealed the presence of at least 71 co-occurrences that provide insight into many natural components used in edible coatings for red fruits, such as proteins, polysaccharides, lipids, phospholipids, and minerals. The review examined their composition, functioning, application techniques, limits, safety considerations, legal regulations, and potential future developments. This review has shown that an edible coating may act as a protective layer on the surface of the fruit, alter the interior gas composition, reduce water loss, and postpone fruit ripening, thereby enhancing the health-promoting properties.

1. Introduction

Consumer behaviors have been greatly influenced by the substantial demand for health-conscious food items. It is commonly recognized that consuming fruits and vegetables provides significant health benefits and helps to protect against many ailments. Cheung et al. (2021) emphasized that consuming a diverse range of fruits and vegetables daily might significantly lower the risk of death [1], particularly in relation to cardiovascular disease, chronic illnesses, and cancer [2]. Red fruits, commonly referred to as berries, are diminutive fruits which are highly valued for their crimson color, tangy-sweet flavor, and advantageous effects on well-being [3]. There are several types of red fruits, but the most important ones are from the Rosaceae (strawberries, sweet cherries, raspberries, and blackberries), Ericaceae (blueberries, cranberries), Myrtaceae (Jaboticaba), and Arecaceae (Açai) families. These fruits are rich in essential nutrients and have low caloric contents. They may be consumed either in their raw form or processed into various products, such as juice, fruit pulp, jams, sweets, and fermented drinks [3].
Berries with a certain color, including blackberries (Rubus species), black raspberries (Rubus occidentalis), blueberries (Vaccinium corymbosum), cranberries (Vaccinium macrocarpon), red raspberries (Rubus idaeus), and strawberries (Fragaria ananassa), are frequently consumed by humans either in their natural state or after undergoing processing [4]. Red fruits are high in phenolic chemicals, with the main types being phenolic acids (hydroxycinnamic and hydroxybenzoic acids), flavonoids (flavanols, flavonols, and anthocyanins), and stilbenes (resveratrol). These are distributed in varying amounts in different regions of the fruit (epicarp, endocarp, and seeds). Furthermore, while polyphenols may be found in many plant matrices, red fruits are known to contain a high concentration of anthocyanins, which are responsible for their color [3]. Hence, the popularity and consumption of red fruits have experienced substantial growth. This can be attributed to their rich nutritional content, distinct taste, flavor, and beneficial properties for health promotion. Red fruits are recognized as dietary sources of bioactive compounds, which contribute to their known health benefits [5,6,7,8].
In red fruits, flavonoids, specifically anthocyanins, play a crucial role in determining their color, and they possess various beneficial effects such as antioxidant, antimicrobial, anti-inflammatory, anticancer, and cardioprotective properties [9]. However, these compounds are prone to degradation during plant growth, food processing, and gastrointestinal processes, which ultimately reduces their bioaccessibility [3]. Consequently, the primary obstacle in distributing high-quality berries concerns the need to prevent their deterioration and prolong their storage capacity. Berry fruits are particularly vulnerable to pathogen attack, over-ripening, excessive respiration, softening rate, susceptibility to mechanical damages, and decay [4,9]. The most effective techniques for preserving quality during the postharvest period are immediate precooling and storage at low temperatures. Manipulating and regulating the composition of gases in the environment, specifically increasing carbon dioxide (CO2) levels up to 20 kPa and oxygen (O2) levels between 5 and 10 kPa, may inhibit the development of microorganisms and delay the senescent process. However, these modified atmospheres may also have an impact on the presence of bioactive chemicals, and the specific reaction to these technologies varies depending on the cultivar [9]. Therefore, to mitigate the loss of red fruits and prolong their storage duration, it is necessary to investigate novel preservation techniques [10]. Hence, the utilization of edible coatings is a contemporary approach to preserve the quality of red fruits and decrease food waste [11,12]. It serves to prolong the postharvest lifespan and mitigate the deterioration of quality [13]. Moreover, it presents an environmentally friendly substitute to synthetic packaging materials [10,14].
Edible coatings are composed of food-grade ingredients that are safe for human consumption, and are formed into thin, semi-solid films [15]. These are specifically engineered to possess a range of functional characteristics that prevent the decay of fruits during storage and distribution. These characteristics include safeguarding against physical damage, regulating the movement of vapor and fluids, and creating barriers against UV (ultraviolet) and visible light [16]. In addition, they may be modified to include preservatives (such as antimicrobials or antioxidants) that extend the shelf life (“active packaging”) or sensors that detect changes in food quality, freshness, or safety (“smart packaging”) [15,17]. Food surfaces may be coated with a homogeneous layer of the required thickness through the use of methods such as panning, brushing, spraying, or dipping [18]. For edible films and coatings, it is important to use substances that are considered safe for consumption. These substances should be considered “generally recognized as safe” (GRAS) by the Food and Drug Administration (FDA) in the United States or by equivalent regulations in other jurisdictions [15,18]. In recent years, there has been a significant effort to produce effective and economically viable edible coatings as an alternative to petroleum-based coatings in order to address related issues [15,19]. Specifically, there has been an increase in consumer apprehension over the adverse ecological effects of non-biodegradable single-use plastics [16]. Natural edible coatings may address these issues and provide further advantages, such as improved health via nutritional fortification [15,20]. Hence, the advancement of edible coatings that might improve the quality and prolong the shelf life of red fruits may lead to increased consumption, thereby resulting in positive health outcomes.
The objectives of this review were to (i) analyze the health benefits of red fruits, (ii) evaluate the postharvest management and technological uses of red fruits, (iii) examine the existing literature on the potential applications of innovative edible coatings for red fruits, focusing on their ability to maintain the quality of bioactive compounds, prevent degradation, and reduce microbial contamination, and (iv) present scientific evidence concerning the effects of various sustainable and healthy edible coatings on the quality of bioactive compounds, sensory acceptability, antimicrobial properties, and shelf life of red fruits, with the aim of minimizing quality deterioration.

2. Materials and Methods

A literature review was performed for English language articles in Scopus to search for published articles relating to the application of natural edible coatings on red fruits and the impact on their bioactive content and shelf life. To gather scientific documents on the topic, the following search terms were used in the article titles, abstracts, and/or keywords of Scopus databases, using English as the filter language: “red AND fruits, AND health AND promoting, AND natural AND edible AND coating, AND bioactive, AND shelf life”. The collected documents obtained through the scientific database were then manually analyzed via a reference manager tool (Zotero version 6.2) to exclude duplicates and any documents not relevant in food processing and human nutrition. The keywords analysis was based on both “all keywords” in the titles and abstracts of the collected documents and the “authors keywords”. VOSviewer was used for the bibliometric mapping [21]. In this latter case, a co-occurrence network was built considering a minimum number of five occurrences for each keyword.

3. Results

3.1. Bibliometric Analysis

Bibliometric data outcomes of the scientific search in Scopus databases, using the keywords (red AND fruits, AND health AND promoting, AND natural AND edible AND coating, AND bioactive, AND shelf life) returned 132 scientific articles published between 2013 and 2024. These articles mainly defined the terms “red fruit health benefits,” “edible coating”, “benefits”, “application method”, and “influence on bioactive compounds and shelf life”. There were eighteen subject categories in all, and, for nine of them, at least seven publications could be located. Engineering and agricultural and biological sciences were the most popular topics in edible coating research, as shown in Figure 1, which lists the top 10 research areas based on the quantity of publications. These two fields combined made up 60% of all articles. Scientists’ principal attention is thus on redesigning naturally edible coatings and contrasting them with conventional coatings.
Figure 1. Bibliometric data: documents by subject area.

3.2. Keywords Clustering

The co-occurrence network of keywords shown in Figure 2 was acquired using VOSviewer version 1.6.18. The examination of the keywords included in the articles that were retrieved provides useful assistance, as does its visual depiction as a co-occurrence network (Figure 2). From the 136 scientific publications available in the Scopus databases, a total of 1364 keywords were obtained. These keywords were then sorted and categorized according to how frequently they appeared in the red fruits and edible coating topic. Figure 2, which shows the network of terms with at least five occurrences, provides important information. There were seventy-one keywords in all, arranged into four clusters. The first group was symbolized by red color and was primarily concerned with health-promoting qualities. It included terms such as bioactive compounds, antioxidants, functional foods, carotenoids, health benefits, fruits, vegetables, and food waste. This cluster depicts the variability and diversity of red fruits studied by scientists and nutritionists to better understand their influence on human health. The green color helped us to identify the second cluster, which is primarily concerned with food preservation and shelf life, using terms such as food packaging, active packaging, packaging materials, food manufacturing, food preservation, nanotechnology, and their influence on food safety and quality. This demonstrates that one of the primary goals of scientific study was to link edible coatings to shelf life and bioactive content preservation. The inclusion of the term “nutraceuticals” is of major value in characterizing the health-promoting features of this cluster, which is closely associated with red fruits. The blue cluster focuses on the production and application of edible coatings, with keywords including chemistry, biochemistry, spray drying, electrospinning, and encapsulation. The smallest cluster, in yellow, comprised terms related to natural edible coating and sustainability, including proteins, plant extracts, biological characteristics, metabolism, sustainable development, chitosan, biopolymers, and biodegradability. This cluster serves as a junction/continuity point for the red, green, and blue clusters, all strongly connected to subjects relating to sustainable food processing and nutrition.
Figure 2. Network of keywords frequently co-occurring in Scopus databases regarding red fruits and edible coating.

4. Discussion

4.1. Red Fruits: Health-Promoting Properties and Benefits

The term “red fruits” refers to a collection of fruits that are either black or red in color, often in the form of berries. Examples include strawberries, cherries, red raspberries, black raspberries, blackberries, cranberries, blueberries, blackcurrants, and grapes [5]. Red fruits are widely acknowledged for their abundance of essential nutrients, such as vitamins (A, C, and E), minerals (calcium, phosphorus, iron, magnesium, potassium, sodium, manganese, and copper), as well as dietary fibers and antioxidants, particularly anthocyanins and ellagitannins [5,6,7,22]. Vitamin C, also known as ascorbic acid, is the predominant chemical found in red fruits and serves as a primary antioxidant in this fruit category. Blueberries have the highest concentration of ascorbic acid among fruits, whereas raspberries have a comparable amount to strawberries, and blackberries have a lesser concentration [5,23]. Cranberries have been recently classified as a novel functional food and nutraceutical, being recognized for their distinctive role in preserving urinary tract health [24].
Red fruits are rich in phenolic compounds, specifically phenolic acids (hydroxycinnamic and hydroxybenzoic acids), flavonoids (flavanols, flavonols, and anthocyanins), and stilbenes (resveratrol), which are the primary classes of phenolic compounds found in these fruits [5]. Similarities in the phenolic content can be observed among red fruits belonging to the same plant family and genus. Flavonols and hydroxycinnamic acids are commonly found in the Ericaceae family, specifically in the Vaccinium genus (which includes bilberries, blueberries, cranberries, and lingonberries) [5,25,26,27,28,29]. On the other hand, gooseberries, black currants, and red currants (which belong to the Grossulariaceae family, genus Ribes) are mainly characterized by the presence of flavonols [30,31]. Ellagic acid is the primary phenolic compound found in fruits belonging to the Rosaceae family, namely the species Fragaria and Rubus (strawberries and red raspberries) [31,32,33]. Although red fruits may seem identical, each species has its own characteristics. As an example, Häkkinen et al. documented that blueberries have significant amounts of quercetin and caffeic acid [34], while bilberries and lingonberries retain lower levels of quercetin [5]. Phenolic compounds possess antioxidant capabilities that hinder the spread of free radicals by stabilizing them via donating a single electron or a hydrogen atom [35,36]. Hence, consuming red fruits with antioxidant characteristics on a regular basis lowers the levels of RONS in the body, hence reducing the likelihood of developing the mentioned illnesses [3,37,38]. For instance, Huang et al. demonstrated that strawberries contain abundant anti-inflammatory phenolics, specifically anthocyanins [39]. These compounds have been proven to reduce the inflammation and oxidative stress that occur after a meal in overweight individuals who are otherwise healthy. This effect is particularly pronounced when the strawberry drink is consumed prior to the meal, as indicated by Cosme et al. [5].
Also, anthocyanins are widely recognized as the predominant and most significant category of phenolic compounds found in red fruits, responsible for their blue, purple, red, or black pigmentation [5]. Furthermore, the composition of anthocyanins in fruits and their extracts is associated with several biological characteristics, particularly in reducing the likelihood of multiple illnesses caused by oxidative damage to cells [40,41,42,43,44]. In addition, they possess antibacterial characteristics, as shown by Widyarman et al. [45], and antiviral activities, as shown by Figueira et al. [46]. Moreover, these chemicals play a crucial role in determining the distinct sensory characteristics of red fruits and facilitating the development of diverse industrial technologies. The efficacy of red fruits in combating metabolic illnesses has been shown via rigorous testing on both animal models and people, as shown via the findings presented in Table 1 [3]. Studies have shown that red fruits like acai and jaboticaba may effectively regulate metabolic syndrome by lowering fasting glucose, insulin, and total cholesterol levels [3,47]. Additionally, these fruits have been found to have positive effects on various health issues [42]. The regular consumption of fruits that are high in anthocyanins might potentially lower the chance of developing many forms of cancer, thanks to their antioxidant characteristics [43,44,48].
Table 1. Scientific evidence of the benefits of the health-promoting properties of red fruits.
Research has shown that acai can prevent the growth of cancerous cells in the brain [48]. Similarly, blackberries have been found to inhibit the growth of human colorectal cells [44]. Blueberries have been shown to have a similar effect on acute myeloid leukemia (AML) [43]. Additionally, strawberries have been found to be effective against colorectal cancer [52]. Recent research showed that consuming one cup of blueberries and two cups of strawberries per day effectively decreased inflammation in human blood [3]. Hence, red fruits may serve as a viable natural option for mitigating the antecedents of several inflammatory conditions [53]. Anthocyanins and other phenolic compounds derived from red fruits, such as blackberries [46], acai [50], and blueberries, have been linked to neuroprotective benefits, including the prevention of dementia and the enhancement of cognitive function [40]. The neuroprotective properties of red fruits, particularly those rich in flavonoids, have been investigated in scientific studies. Quercetin, a flavonoid found in red fruits like berries, citrus fruits, and red wine, has demonstrated neuroprotective effects. It acts as an antioxidant and anti-inflammatory agent, contributing to the prevention of various diseases [78]. Additionally, anthocyanins, another type of flavonoid present in red fruits, can penetrate the blood-brain barrier and offer potential neuroprotective benefits [79]. Bortolini et al. found that concentrations of 20 ug/mL of blackberry, black raspberry, blueberry, cranberry, red raspberry, and strawberry extracts were effective in preserving the cellular viability of bv-2 microglia when exposed to oxidative stress [3]. These concentrations reduced the regulation of caspase 3/7 activity by 35.5%, 40.7%, 46.3%, 54.9%, and 46.9%, respectively. The study conducted by Shao et al. demonstrates the neuroprotective effects of blueberry anthocyanins [80]. These effects include protection against neural morphology changes caused by perfluorooctane sulfonate, alterations in neurotransmitter levels, changes in neural-related gene expression levels, and DNA damage. The primary association of this protective mechanism is in the capacity of anthocyanins to diminish the presence of free radicals. Nevertheless, a more comprehensive understanding of the intricate neuroprotective processes arising from phytochemicals found in red fruits is necessary [3]. Due to the numerous health benefits provided by berry fruits, red fruits can be regarded as a natural substitute for managing various chronic illnesses [3]. Additionally, they have the potential to be utilized in the development of innovative technologies for industries [81,82,83,84].

4.2. Red Fruit: Postharvest Management and Technological Applications

The handling of red fruits presents a range of difficulties. Berries are devoid of a safeguarding outer layer and are very prone to spoilage, mostly due to their vulnerability to physical harm, dehydration, and fungal deterioration [85]. In addition, berries are classified as non-climacteric fruits, meaning that they must be picked when they are fully mature or very close to it. Once separated from the plant, most berries will not continue to ripen naturally, and their eating quality will not increase after harvest [9]. Under some circumstances, fruits may undergo coloration during storage. However, if they are picked prematurely, their texture, sweetness, and acidity may not reach complete development [9,86]. The quality of fruit for the market is primarily assessed based on physicochemical factors such as size, full color, gloss, a firm and crisp texture, a lack of decay, injuries and bruises, a harmonious balance between sweetness and acidity, green sepals, and a distinctive perfume [9]. The primary factors contributing to loss and rejection include weight reduction, the appearance of bruises and cuts, signs of mildew and decay, alterations in color, juice leaking, and sepal wilt [9]. The level of fruit maturity at harvest and the way the fruit is handled are two crucial determinants of the postharvest preservation quality. The level of ripeness at harvest significantly impacts the shelf life of the berries, their storage characteristics, and the likelihood of sales [87]. Immature fruit may have a greater capacity for storage, but they are unlikely to acquire the desired sensory features. On the other hand, over-mature fruit usually has a limited shelf life due to an increased susceptibility to decay [9,88]. To achieve the highest level of quality at the time of harvest and preserve this quality during transportation and commercialization until the fruit is eaten, it is crucial to pick berries when they have reached the ideal level of maturity [9,89]. To prevent excessive handling and harm to the fruit, it is recommended to manually collect, sort, classify, and pack berries in the field straight into the final container for the purpose of selling them while fresh.
Red fruits are used for several industrial uses, being processed into food and drinks as well as medicinal and nutraceutical items. Red fruits are highly valued for their perishability, phenolic content (particularly anthocyanins), and sensory qualities, making them desirable for both fresh consumption and industrial processing [3]. Therefore, there is a growing market for fruits that have undergone little processing, have been sterilized, peeled, and sliced, and are ready to be consumed [82]. Red fruits are utilized in the production of various fruit-based products, including juices, wines, non-alcoholic beverages, dairy products, fermented beverages like kombuchas, purees, pulps, desserts, jellies, and different types of vinegar [90,91,92,93]. Furthermore, novel technologies catering to the chemical, pharmaceutical, and food sectors have been developed, alongside their traditional uses. Red fruit growers may capitalize on this market by offering several solutions to enhance the value of the raw material [3]. The traditional method of processing fruits into juice and fermented drinks generates a significant quantity of waste or by-products, particularly fruit pomaces. These pomaces are composed of leftover pieces of fruits, such as epicarp, endocarp, mesocarp, and seeds. The majority of fruit pomace is comprised of the fruit’s endocarp and seeds [3]. Consequently, this leftover material has a high concentration of carbohydrates and dietary fibers, as well as various phenolics that may be extracted for industrial purposes [94,95]. In addition, food compositions such as cookies also include fruit pomaces, sugars, and dietary fibers [96]. Consequently, the use of fruit pomaces might facilitate the creation of novel environmentally friendly products that possess the advantageous characteristics of the enhanced chemicals derived from red fruits [3].
Red fruits have a short shelf life because they contain a lot of water, have significant postharvest activity, and are prone to fruit rot and darkening [9]. The elevated respiration rates of these fruits result in modifications in texture, color, taste, and nutritional composition during storage. These alterations play a vital role in assessing fruit quality and customer satisfaction [97]. Their limited shelf life is also attributed to deterioration induced by rot-causing bacteria and rapid softening rates [98]. Ethylene in storage may enhance respiration rates and promote the formation of gray mold. Water loss is a physiological issue that may impact berries during storage. This leads to fruit shriveling, a loss of gloss, and has a significant influence on the degradation of anthocyanin [9]. The process of fruit aging is hastened by water loss, and the fruit becomes unsuitable for sale after it has lost more than 6% of its weight [9]. To minimize water loss during the postharvest handling of berries, it is important to promptly precool them and store them in appropriate packaging at the optimal temperature and relative humidity [9,99].
Postharvest washing, immediate quick chilling, and storage at low temperatures are the primary procedures used to preserve the quality and stability of bioactive chemicals, and to prevent the deterioration of fruits and vegetables [9,100,101]. In addition, postharvest infections are often managed using synthetic fungicides [102] and by keeping the produce in controlled or modified atmospheres with elevated levels of [9,103]. Nevertheless, these approaches do have some constraints. It is not advisable to wash berries before selling them since it may easily damage the peel, and the time it takes to dry them slows the cooling process and increases the risk of infection by harmful germs [102]. In addition, chemical fungicides can have various adverse impacts on both food safety and the environment. This raises concerns among the public regarding environmental pollution, the potential contamination of berries due to fungicide residues, and the challenge of controlling fungal diseases caused by the emergence of fungicide-tolerant strains of pathogens [9,104].

4.3. Study on Red Fruits and the Implementation of Cutting-Edge Edible Coating

Pham et al. state that the primary purpose of developing edible coatings was to substitute and reduce the use of potentially hazardous chemicals and synthetic substances that might pose risks to consumers’ well-being [105]. An edible coating is a thin layer made from biological or chemical components that is applied to the surface of a product. It may be constituted by a single layer or by many layers [106]. Edible coatings aid in preserving phytonutrients (such as antioxidants, phenolics, and pigments) and regulating the physicochemical properties (such as respiration rate, weight loss, total dissolved solids, and pH) of fruits over an extended period [107]. Consequently, the decay of fruit is postponed, the quality of fruit is maintained, and the duration for which fruit remains fresh is prolonged [108,109]. To be efficient, edible coatings must fulfil various functional criteria, including the following: (i) being free of toxic substances and safe for human consumption; (ii) exhibiting exceptional barrier properties with respect to water, humidity, O2, CO2, and C2H4; and (iii) enhancing the visual and textural characteristics of the coated products [110]. The application of the coating must not modify the sensory characteristics of the fruit [105,111]. Hence, it is important to meticulously develop the formulation of the edible coating throughout its creation. Moreover, the coating must regulate the gas exchange to prevent fruit fermentation and the development of unpleasant off-flavors [105,112].

4.3.1. Edible Coatings and Their Operational Principles

An edible coating is a thin layer made from edible substances and applied to food items to extend their shelf life [113,114]. Currently, there is a growing interest in edible coatings and films compared to other synthetic plastic packaging materials, largely since they are biodegradable and can be consumed. They are also compatible with food [115,116,117]. Edible coatings are often derived from biodegradable polymers as a substitute for nondegradable packaging materials that generate significant waste [107,118]. Edible films and coatings are a viable technological solution for preserving food quality, ensuring safety, and enhancing usefulness [10]. To be considered suitable, the materials must fulfil certain criteria, such as exhibiting favorable sensory characteristics, possessing effective barrier qualities, demonstrating sufficient mechanical resilience, maintaining biochemical, physicochemical, and microbiological stability, ensuring safety for human consumption, being environmentally friendly, and being reasonably priced [10]. Edible coatings are produced using natural raw ingredients, including polysaccharides, proteins, lipids, and their mixtures. In addition to their ability to preserve food, edible coatings are designed with chemicals, such as antioxidants, flavorings, and sweeteners, to achieve active packaging and improve the nutritional and sensory characteristics of the food [119,120].
Edible coatings and films can maintain the visual appeal, firmness, moisture content, and can extend the storage duration of fruits. This is due to their ability to act as barriers against moisture and gas transmission, prevent lipid oxidation, regulate enzymatic activities, and inhibit microbial spoilage [10,112,121]. Edible coatings alter the surrounding environment of fruits by modifying the gas composition inside the fruit, thus slowing down the respiration rate and ethylene generation. This effectively reduces the physiological degradation of fruits [4]. Moreover, it has been shown that edible coatings including antimicrobial substances, such as organic acids, plant essential oils, and polypeptides, effectively hinder the proliferation of microbes [122]. Edible coatings can decrease enzyme activity, minimize the occurrence of browning reactions, and prevent texture softening [10]. Furthermore, it has the capacity to preserve the inherent volatile flavor and color components [123]. The edible coatings shield food products from microbial contaminants, diminish the effects of deterioration [124], extend the storage duration [125,126], and minimize lipid oxidation and moisture loss in food products [10,127,128].

4.3.2. Formulation of Edible Coating for Red Fruits Application

Edible coatings are primarily categorized into three primary types. Coatings can be made from various types of materials, including polysaccharides (such as starch, chitosan, cellulose, alginate, pectin, and gums), proteins (such as zein, whey protein, wheat gluten, casein, soy protein, egg albumin, and gelatin), lipids (such as waxes and fatty acids), and composite materials. Moreover, coatings can be formed by combining multiple substances together [129,130]. Table 2 provides a summary of the several edible coatings that have been applied to red fruits, as documented in the literature.
Table 2. Composition of edible coatings for red fruit applications with their impact on the quality attributes of berries.
Polysaccharides are naturally occurring polymers that are used in the production of edible coatings. Polysaccharide-based coatings for food preservation often use starch, gums, and chitosan as fundamental constituents [130]. The use of these coatings offers several benefits, such as cost-effectiveness and enhanced accessibility. Although several polysaccharides exhibit reduced water vapor barrier properties, polysaccharides like alginate and carrageenan are notably hygroscopic and possess thick film characteristics. The polysaccharide-based edible coating presents both antioxidant and antibacterial properties [130].
Protein-based edible coatings are derived from both plant and animal sources. Examples of plant-based protein coating materials include zein (derived from maize), soy protein, gluten (obtained from wheat), and other similar substances. On the other hand, animal-based protein sources encompass whey protein, milk protein casein, egg albumin, and gelatin [130]. They exhibit a significantly greater ability to create a barrier against mechanical strength, organoleptic and aroma retention, and high oxygen permeability. However, they do not possess a moisture barrier due to their hydrophilic properties, which can be enhanced by incorporating hydrophobic substances like lipids [130].
Edible coatings made from lipids, such as acetylated monoglycerides, waxes, vegetable oils, or minerals, enhance the appearance of fresh fruit by giving it a shiny and glossy appearance. Lipid coatings, being hydrophobic, may effectively mitigate the effects of oxygen, water, light, and other environmental factors on the quality of stored products. Additionally, they can reduce the rate of water evaporation from food items [130]. In addition, they provide protection against the detrimental effects that mostly occur in cold storage facilities [18,130].
In a recent study by Khalid et al. [130], there has been a significant focus on developing bilayer or composite coatings that include polysaccharides, proteins, and/or lipids. The aim was to enhance the functional quality of these coatings. Every covering substance has a unique but limited role. The combination of two different coating ingredients may enhance the functionality. The main purpose of composites is to maximize the performance of the combination while maintaining the stability of the individual components [130]. The combination of several materials may lead to the development of composite edible coatings that possess distinctive characteristics.

4.3.3. Application Technique for Applying an Edible Coating on Red Fruits

Different procedures may be used to apply edible coatings onto red fruits, depending on the state of the coating ingredient, which can be a liquid, suspension, emulsion, or powder. The choice of an effective coating method influences not only the preservation effect of the coatings created on the food products, but also the manufacturing cost and process efficiency [156]. Table 3 summarizes the major different coating methods with their advantages and disadvantages. The selection of an appropriate application procedure is crucial to maximize the efficacy of the coating and produce the most promising outcomes [157].
Table 3. Main process parameters, benefits, and drawbacks of edible coating application techniques.
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Immersion (Dipping Coating Method)
The immersion technique is widely used due to its ease of implementation and cost-effectiveness. This process involves the application of a liquid, suspension, or emulsion coating. The process consists of the following three specific stages: (i) immersion and residence, (ii) deposition, and (iii) solvent evaporation [105,157,167]. To begin, submerge the food in the coating at a consistent speed, ensuring that the food contacts and retains the coating on its surface [157]. Deposition is a process used to acquire tiny layers of coating. In this process, the food is immersed and then kept still to allow the surplus coating to be eliminated via deposition [168]. The last stage, solvent evaporation, entails the removal of any surplus coating by means of either drying equipment or natural atmospheric conditions, thus allowing for the evaporation and drying of the food [157]. The immersion period might deviate from 5 to 30 s depending on the food product and edible coating purpose [169]. Prior research has demonstrated that the density and structure of the coatings formed through the process of dipping are greatly influenced by various factors. These factors include the duration of immersion, the speed at which the object is withdrawn, the number of dip-coating cycles, the properties of the coating solution, such as density, viscosity, and surface tension, the characteristics of the substrate surface, and the conditions during the drying process [105,170]. This approach has several benefits, since the whole surface of the food is consistently coated, and it may be applied to diverse surfaces. Nevertheless, the dipping approach has certain drawbacks. It may not be good for fruits with sensitive skins or membranes, since they may be harmed during immersion [171]. Dipping commonly results in a thick layer, leading to substantially reduced fruit respiration and potentially accumulate unwanted residues, and thus resulting in the development of microorganisms within the coating itself [157,160], damage food surfaces, and degraded function [105]. In addition, germs and debris from the fruit surface may contaminate the coating solution, thereby demanding industrial up-scaling. Another downside of the dipping strategy is the considerable amount of solution required for coating per unit mass of the product to assure proper dipping conditions [105,111].
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Spreading Technique
The spreading method is excellent for coating solutions with a high viscosity. In general, the wetness level and spreading rate are the major criteria used to characterize how the coating solution is dispersed across the food surface [105]. Several elements impact the efficacy of coating deposition via spreading, including the substrate quality, especially the drying conditions, liquid properties, and surface geometry [105,172]. This approach is employed for covering fruit with a tiny surface area, such as grapes [171,173]. The coating substance is applied to the surface of the fruits using a brush by skilled operators [171]. Thus, the human component has a substantial influence on the quality of the coating and thickness uniformity [105].
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Spraying Methods
The edible coating material may also be sprayed to the surface of red fruits using a spray nozzle via spraying technique [171,174]. Spraying is the procedure of utilizing a collection of nozzles to disperse tiny coating droplets over the fruit surface [105,157]. There are several types of spraying, such as air spray atomization, which involves low speed and cylindrical spray flow, air-assisted airless atomization, used for highly viscous coatings, and pressure atomization spraying, where coatings are applied using pressure instead of air [105,157,160]. The biopolymer is dissolved into a suitable solvent in order to generate a solution. The solution is then atomized into a fine mist using a spray nozzle, which is sprayed onto the surface of the fruit or vegetable [171,175]. When spraying, the pressure, viscosity, surface temperature, and coating tension are critical elements that greatly determine the final coating performance [157,160,176]. The benefit of spraying is that it is a rapid and effective approach that can be readily scaled up for commercial production [171] and to have the capacity to apply two or more distinct coatings, thus generating a multi-layered coating [160]. Additionally, the thickness of the coating layer may be adjusted with this process [157]. Adding antibacterial agents or antioxidants to the biopolymer solution may help to increase the coated produce’s shelf life [171]. The spraying method permits multi-layer applications such as interlayer solutions, as well as uniform coatings with homogenous thicknesses [177]. Additionally, the coating thickness is larger than that when using the dipping technique, owing to the low viscosity of the solution [105,160]. However, the spraying approach also has significant disadvantages [178].
➢
Pan coating technique
The pan coating process comprises putting the food products within a revolving container, where the coating is subsequently sprayed or sprinkled, thus guaranteeing a homogenous integration onto the food surface [157]. Subsequently, the coating is cured [179]. The pan coating process is used to manufacture thin or thick layers of coatings onto hard surfaces, enabling for the simultaneous coating of many food items, even with size variability, thus providing a clear, flexible, and shiny coating material [157]. The primary drawback of this technology is that it takes some time to achieve, considering the necessity for water evaporation throughout the process [157]. Moreover, the coating must be applied gradually; otherwise, it may cause the food items to cling together within the revolving container [157,165].
➢
Electro-spraying
Electro-spraying is a process in which a liquid is atomized into small droplets using an electric field. The electro-spraying method utilizes a strong electric field to produce charged droplets that have a precise and limited size distribution. These droplets are of micrometric and sub-micrometric dimensions [180]. The electro-spraying approach may modify droplet characteristics, such as size and layer thickness, by adjusting the flow velocity and solution viscosity [105,181].
➢
Vacuum Infusion
Vacuum infusion is used for fresh fruits and vegetables that have a porous structure [112,171]. The biopolymer solution is injected into the vacuum chamber, where it permeates the pores of the fruits [182]. Implementing this technique on food items like carrots has the potential to enhance their sensory properties and nutritional content, while also prolonging their shelf life and maintaining their firmness. This might be particularly advantageous for the preservation of fruits with robust skins or membranes that need a more substantial coating [183]. Vacuum infusion is not suitable for fruits with delicate structures as they may be damaged during the vacuum process [171,184].
➢
Layer by Layer (Multilayer Coating)
The layer-by-layer deposition technique relies on the electrostatic interactions between the food surface and the charged polyelectrolytes. The electrostatic interactions enhance the attachment of the coating to the food surface and may be used to produce coatings which consist of several thin layers that are chemically or physically interconnected [105]. Each layer has a distinct function, such as avoiding the infiltration of pathogenic organisms into the fruit or restricting the oxygen supply [171,185]. The efficacy of such connected multilayer coating is enhanced in comparison to typical edible coatings [105,186]. The use of a multilayer coating technique to enhance the density of coating layers during the storage of fruits after harvest has been recorded for polysaccharides and charged polyelectrolytes that can form hydrogen and covalent bonds. Polysaccharides and charged polyelectrolytes have been shown to be effective in preserving fruits when using the multi-layer coating technique to enhance the integrity of the coatings [105,187]. The efficacy of the multilayer coating technique in prolonging the storage duration of various types of fresh produce, such as strawberries, has been shown. The approach is ecologically sustainable as it minimizes the reliance on chemical preservatives and packing materials [171,188].
➢
Solution Casting Process
The process of solution casting for the application of bio-coatings entails dissolving the biopolymer in an appropriate solvent and then putting it onto the surface of the fruit [189]. Solution casting is a technique used to produce independent films inside a Petri dish or a similar container. Dipping and spraying are the predominant techniques used to apply substances to fruits, with the solvent, often water, evaporating while the fruit undergoes drying. Prudent consideration should be given to the selection of the solvent for dissolving the biopolymer, as it must exhibit compatibility with both the biopolymer itself and the fruit or vegetable intended to be coated [171]. Upon the application of the solution onto the fruit surface, the solvent undergoes evaporation, resulting in the formation of a residual thin coating. The thickness of the coating may be controlled via adjusting the concentration of the biopolymer solution or the number of coating layers applied [171,175,189]. The advantage of solution casting lies in its simplicity, cost-effectiveness, and scalability for large-scale commercial manufacturing [190]. Furthermore, the thickness and homogeneity of the coating may vary depending on the application procedure, thereby affecting its efficacy [191]. Ultimately, solution casting is a very promising technique for acquiring bio-coatings that may effectively preserve fruits. This technology has the potential to enhance food quality and minimize food waste, as shown by recent studies conducted by Moeini et al. [192] and Ungureanu et al. [171].
➢
Three-Dimensional Food Printing Method
Three-dimensional food printing is the process of using a three-dimensional printer to sequentially place materials in layers, resulting in the creation of a food item with a distinct structure, texture, and, perhaps, nutritional composition [193]. The use of 3D printing technology has the potential to fabricate accurate and consistent edible coatings on fruits and vegetables, hence improving their durability and overall quality. This has the potential to be used for incorporating natural preservatives or antimicrobials into printed food, hence prolonging the freshness of perishable goods [171]. The use of 3D printing technology may contribute to the mitigation of food waste through enabling the production of edible items from fruits and vegetables that would otherwise be rejected due to their visual flaws. While it does not specifically preserve fresh food, it may effectively enhance the use of gathered fruits and vegetables. Although still in its early stages, 3D printing shows potential in producing biodegradable packaging for fruits and vegetables, particularly red fruits [171]. This might be devised to provide safeguarding and perhaps to integrate conservation methods (e.g., modified atmosphere packing) [194].
➢
Cross-Linked Coating Method
Cross-linking refers to the act of joining polymer chains together via covalent and non-covalent connections [105]. Cross-linking is the chemical bonding process that connects polymer chains, resulting in the formation of a three-dimensional network of interlinked chains [171]. Cross-linked coatings are often formed via applying the coating solution onto the food surface with methods such as spraying, dipping, or spreading. Subsequently, a cross-linking agent is introduced to enhance the density and durability of the coating [105]. This method is employed in the development of coatings to enhance their functionality, as the cross-linked arrangement generally yields enhanced mechanical robustness, resistance to water, and stability [155]. Regarding the preservation of fresh fruits and vegetables, the cross-linked coating approach has several benefits such as enhanced mechanical characteristics, chemical and thermal durability, and improved molecular migration [105,195]. Cross-linked coatings possess denser polymer networks, resulting in decreased gas permeability (e.g., oxygen, carbon dioxide) and water vapor, hence retarding the ripening process and minimizing moisture loss in fruits and vegetables. These coatings generally exhibit enhanced mechanical strength and increased resistance to abrasion or damage, hence assuring the preservation of the protective layer during handling and transportation [171]. Cross-linked coatings exhibit enhanced resistance to dissolution and degradation, resulting in heightened stability and durability throughout extended storage periods. Cross-linked coatings may serve as a framework for enclosing and dispensing active substances, such as antibacterial and antioxidant chemicals [171]. The cross-linked structure enables the regulated release of these substances, thereby improving the longevity and safety of the product. Chitosan, a biopolymer obtained from natural sources, is often used in edible coatings. It may undergo cross-linking via the use of agents like geminin [171,196].
Cross-linking is very efficient for biopolymer materials composed of proteins or polysaccharides [105]. Proteins are preferred over polysaccharides in this approach because proteins have a higher abundance of functional groups (Dai et al., 2020). Nevertheless, it is crucial to emphasize that a comprehensive evaluation of the safety of the cross-linking agents and the possible transfer of compounds from the coating to the food must be conducted in order to guarantee food safety. The coatings must consistently adhere to the relevant food safety norms and standards [171].

4.4. Studies That Examine the Effects of Different Sustainable and Healthy Edible Coatings on the Bioactive Compound Quality, Sensory Quality/Acceptability, Antimicrobial Properties, and Shelf Life of Red Fruits

In search of environmentally friendly substitutes, bioactive compounds derived from by-products can be employed to impart their activities in edible coatings, giving them characteristics like antimicrobial, antioxidant, and anti-enzymatic qualities, as well as therapeutic advantages for health, like being anticholesterolemic and other attributes [197]. Edible films and coatings are frequently made from many basic compounds that are then combined with other compounds to improve their functions [198]. The results of several studies on the application of edible coatings onto red fruits and its effects on the quality of the bioactive components, sensory quality and acceptability, antibacterial capabilities, and shelf life of red fruits are summarized in Table 4.
Table 4. Application of edible coatings onto red fruits and its effects on the quality of the bioactive components, sensory quality and acceptability, antibacterial capabilities, and shelf life of red fruits.

4.5. Constraints, Safety Considerations, Legal Regulations, and Future Developments of Edible Coatings for Red Fruits

In Duguma’s work [10], it is noted that, although edible coatings have many benefits, there are several obstacles that hinder their widespread use in industrial settings. Tahir et al. determined that a high concentration of gum in the edible coating might have a detrimental impact on the sensory perceptions of coated vegetables [199]. In addition to the negative impact on the sensory quality of some edible coatings, the instability of bioactive chemicals, as well as the weak film-forming characteristics and surface adherence, provide additional challenges that restrict the commercial use of Aloe vera gel as an edible coating [200]. The use of essential oil to enhance the antimicrobial characteristics of edible coatings leads to drawbacks such as a limited solubility in water, strong odor, and high tendency to evaporate [201]. Furthermore, the absence of suitable materials possessing the necessary functions, as well as the financial burden associated with acquiring and installing the coating equipment, are additional obstacles that impede the implementation of edible coatings. The hydrophobic properties of most edible packing materials, together with the inadequate control over the temperature and relative humidity, provide obstacles that hamper the widespread use of edible coatings in the industry [10]. Furthermore, the lack of approved standards for the application of diverse edible coatings poses difficulties in terms of regulatory and safety concerns. The use of appropriate edible coatings, the optimization of edible coating concentration, the integration of edible coatings with flavor enhancers, and nanoencapsulation techniques may effectively mitigate some drawbacks associated with edible coatings [10]. Additionally, conducting a washing process at the conclusion of storage has the potential to enhance the taste of the edible coating [202]. Nayak et al. proposed that incorporating nanotechnologies such as nanoencapsulation and multilayer systems into edible coatings might address the current limitations of these coatings [203]. This involves the creation of next generation edible coatings that can release active substances. In a similar vein, Bakhy and Zidan found that incorporating nanoelements into edible coatings effectively extended the shelf life of goods by mitigating the development of pathogens and enhancing the quality of fruits and vegetables [204].
Food safety and regulations differ among nations. As per EU and US standards, edible films and coating might be classified as food components, additives, contact materials, or packaging materials [205]. Matloob et al. [162] stressed in their review that edible coatings should adhere to the necessary requirements for food components, as these rules are essential for ensuring the quality of the edible product [206]. To ensure the quality and safety of edible coatings, it is necessary to use constituents that have been generally recognized as safe (GRAS) according to the regulations set by the Food and Drug Administration (FDA) [18,205]. This is important for maintaining the safety of the product and ensuring that it can be consumed without any concerns [162]. However, it is possible for edible coatings to undergo a metamorphosis into poisonous compounds because of the changes that take place during the process of film development [207]. The use of various cross-linking agents to improve the film characteristics and interactions with gastrointestinal contents may also induce the generation of harmful compounds [208]. The research conducted by Roșu et al. examined the impact of graphene oxide and its derivatives on the cytotoxicity of a methylcellulose-based film on human lungs [209]. The findings revealed that reduced graphene oxide exhibited a lesser toxicity when compared to graphene oxide. Despite the impact of the modification methods and ingredient selection being crucial for ensuring the safety of edible films, it is seldom addressed in research concerning edible films [205]. Certain edible films may include substances that have the potential to induce hypersensitivity reactions. Hence, irrespective of the little quantity used, it is essential to inform the client about the presence of a substantial toxin in edible coating films and provide an appropriate warning [162,210]). Users should evaluate and accept each component used in the coatings based on their sensory perception [211]. Furthermore, it is crucial to consider the toxicity and genotoxicity of edible coatings as a significant issue during assembly, as highlighted by Matloob et al. [162]. Under these conditions, organic oils often used in edible coatings as a germicidal compound, although being GRAS (generally recognized as safe) by the European Commission and the United States, might potentially cause hypersensitivity reactions [212]. Specific guidelines exist for including antioxidants and antimicrobials in food compositions [162,213].
Therefore, as evident from the previous section, although edible coatings have the potential to enhance the quality, shelf life, and safety of fresh and minimally processed fruits, their commercial use remains relatively restricted [205]. There are several factors contributing to this phenomenon as follows: the increased expenses; the challenge of consistently producing consistent coatings that adhere well to the fruit; the subpar mechanical, barrier, and optical characteristics of many edible coatings; the potential for allergenic reactions with protein-based coating materials; undesirable sensory qualities; and the challenges of scaling up to mass production [205]. There are some regions that are expected to see growth soon. Nanotechnology may be used to produce edible coatings that possess enhanced functional characteristics through the integration of organic or inorganic nanoparticles into biopolymer films [205]. The addition of these nanoparticles can be utilized to control and adjust the optical, mechanical, and barrier characteristics of coatings. Furthermore, it can aid in the integration of functional components such as colors, flavors, antimicrobials, antioxidants, vitamins, and nutraceuticals ([205]. Furthermore, layer-by-layer techniques may be used to easily fabricate multilayer coatings with improved functional characteristics. The process of microencapsulation may enhance the long-term viability and safety of probiotic strains, thus allowing them to be integrated into edible coatings [214]. Nevertheless, further investigation is necessary to ensure the economic feasibility of several emerging technologies.
Edible films and coatings extend the shelf life and enhance food quality through protecting against physical and mechanical damage, producing a regulated environment, and functioning as a semipermeable barrier for gases, vapors, and water. Guimarães et al. describe edible coatings [215], as carriers of living microorganisms, as a new strategy for biopreservation and healthier foods. However, improper handling and storage can cause microbial degradation and spoilage. Despite their ability to contain antimicrobial chemicals, coatings that are incorrectly formulated or applied may generate an environment that is conducive to bacterial development. Overall, well-coated fruits exhibited less microbial deterioration [216]. Furthermore, aerobic mesophilic bacteria were dramatically decreased, entirely suppressing mold and yeast development on food surfaces without negatively influencing consumer approval [217].

5. Conclusions

Red fruits contain abundant bioactive components, which have been shown to have significant beneficial impacts on human nutrition and health. These benefits may be attributed to the presence of many health-promoting substances, such as organic acids, phenolics, and sugars. Multiple research projects have been undertaken to examine the efficacy of edible coatings in maintaining the quality and extending the shelf life of red fruits. This review has shown that an edible coating may act as a protective layer on the surface of the fruit, alter the interior gas composition, reduce water loss, and postpone fruit ripening. The primary hurdles in edible coating methods lie in finding materials that may effectively prolong the shelf life of red fruits while preserving their sensory and nutritional qualities. This needs additional study and attention. Additionally, the functional efficacy and nutritional quality of these coatings may be improved via integrating additives such as pigments, tastes, substances that prevent oxidation, substances that inhibit the growth of microorganisms, devices that detect changes, essential nutrients, and substances that provide health benefits. Nevertheless, more investigation is necessary to ascertain novel and more efficient compositions, as well as to develop edible coatings that can be inexpensively manufactured in huge quantities. It is important to consider the potential allergenicity of the coating materials.

Author Contributions

Conceptualization, M.R.L., J.O.G.E. and M.P.; methodology, J.O.G.E.; investigation, J.O.G.E.; writing—original draft preparation, M.R.L., J.O.G.E., M.P. and P.P.; writing—review and editing, M.R.L., J.O.G.E., M.P. and P.P.; visualization, J.O.G.E. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Institutional Review Board Statement

Not applicable.

Data Availability Statement

Not applicable.

Conflicts of Interest

The authors declare no conflicts of interest.

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