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Article

Clinical Relevance of Antibiotic Susceptibility Profiles for Screening Gram-negative Microorganisms Resistant to Beta-Lactam Antibiotics

1
Department of Microbiology, School of Medicine, University of Granada-ibs, 18012 Granada, Spain
2
Department of Microbiology, Hospital Universitario Virgen de las Nieves-ibs, 18012 Granada, Spain
3
Department of Emergency, Hospital de la Agencia Sanitaria Alto Guadalquivir, 14550 Montilla, Spain
4
Department of Investigation, Hospital Universitario Virgen de las Nieves, 18012 Granada, Spain
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(10), 1555; https://doi.org/10.3390/microorganisms8101555
Received: 15 September 2020 / Revised: 29 September 2020 / Accepted: 30 September 2020 / Published: 9 October 2020
(This article belongs to the Special Issue Control and Detection of Multiple Antibiotic Resistant Pathogens)
The increasing resistance to antibiotics is compromising the empirical treatment of infections caused by resistant bacteria. Rapid, efficient, and clinically applicable phenotypic methods are needed for their detection. This study examines the phenotypic behavior of β-lactam-resistant Gram-negative bacteria grown on ChromID ESBL medium with ertapenem, cefoxitin, and cefepime disks, reports on the coloration of colonies, and establishes a halo diameter breakpoint for the detection of carbapenemase-producing bacteria. We studied 186 β-lactam-resistant Gram-negative microorganisms (77 with extended spectrum beta lactamase (ESBL), 97 with carbapenemases, and 12 with AmpC β-lactamases (AmpC)). Susceptibility profiles of Gram-negative bacteria that produced ESBL, AmpC, and carbapenemases were similar to the expected profiles, with some differences in the response to cefepime of ESBL-producing microorganisms. Coloration values did not differ from those described by the manufacturer of ChromID ESBL medium. In the screening of carbapenemase production, inhibition halo diameter breakpoints for antibiotic resistance were 18 mm for Enterobacterales and ertapenem, 18 mm for Pseudomonas and cefepime, and 16 mm for Acinetobacter baumannii and cefepime. This innovative phenotypic approach is highly relevant to clinical laboratories, combining susceptibility profiles with detection by coloration of high-priority resistant microorganisms such as carbapenemase-producing A. baumannii, carbapenemase-producing Pseudomonas spp., and ESBL and/or carbapenemase-producing Enterobacterales. View Full-Text
Keywords: beta-lactam antibiotics; resistance; Enterobacterales; Pseudomonas; Acinetobacter beta-lactam antibiotics; resistance; Enterobacterales; Pseudomonas; Acinetobacter
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MDPI and ACS Style

Montiel-Riquelme, F.; Calatrava-Hernández, E.; Gutiérrez-Soto, M.; Expósito-Ruiz, M.; Navarro-Marí, J.M.; Gutiérrez-Fernández, J. Clinical Relevance of Antibiotic Susceptibility Profiles for Screening Gram-negative Microorganisms Resistant to Beta-Lactam Antibiotics. Microorganisms 2020, 8, 1555. https://doi.org/10.3390/microorganisms8101555

AMA Style

Montiel-Riquelme F, Calatrava-Hernández E, Gutiérrez-Soto M, Expósito-Ruiz M, Navarro-Marí JM, Gutiérrez-Fernández J. Clinical Relevance of Antibiotic Susceptibility Profiles for Screening Gram-negative Microorganisms Resistant to Beta-Lactam Antibiotics. Microorganisms. 2020; 8(10):1555. https://doi.org/10.3390/microorganisms8101555

Chicago/Turabian Style

Montiel-Riquelme, Francisco, Elisabeth Calatrava-Hernández, Miguel Gutiérrez-Soto, Manuela Expósito-Ruiz, José M. Navarro-Marí, and José Gutiérrez-Fernández. 2020. "Clinical Relevance of Antibiotic Susceptibility Profiles for Screening Gram-negative Microorganisms Resistant to Beta-Lactam Antibiotics" Microorganisms 8, no. 10: 1555. https://doi.org/10.3390/microorganisms8101555

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