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Volume 13
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10.3390/microorganisms13092045
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Article

New Marine Actinobacteria Strain, Micromonospora sp. SH-82: Characterization, Specialized Metabolites and Biological Activities

by
Alexandre Le Loarer
1,
Laurence Marcourt
2,3,
Rémy Marcellin-Gros
2,3,
Laurent Dufossé
1,
Chatragadda Ramesh
4,5,
Maile Anwesh
6,
Jérome Bignon
7,
Michel Frédérich
8,
Allison Ledoux
8,
Emerson Ferreira Queiroz
2,3,
Jean-Luc Wolfender
2,3,
Mireille Fouillaud
1,* and
Anne Gauvin-Bialecki
1
1
Laboratory of Chemistry and Biotechnology of Natural Products, Faculty of Science and Technology, University of La Réunion, 15 Avenue René Cassin, CS 92003, CEDEX 09, 97744 Saint-Denis, France
2
Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, CMU-Rue Michel-Servet 1, CH-1211 Geneva, Switzerland
3
School of Pharmaceutical Sciences, University of Geneva, CMU-Rue Michel-Servet 1, CH-1211 Geneva, Switzerland
4
Biological Oceanography Division, National Institute of Oceanography (CSIR-NIO), Panaji 403004, Goa, India
5
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, Uttar Pradesh, India
6
DBT-Centre for Microbial Informatics, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad 500046, Telangana, India
7
Institute of Chemistry of Natural Substances (ICSN), Centre National de la Recherche Scientifique (CNRS) UPR 2301, Université Paris-Saclay, 1, av. de la Terrasse, 91198 Gif-sur-Yvette, France
8
Pharmacognosy Laboratory, Department of Pharmacy, Centre Interfacultaire de Recherche sur le Médicament (CIRM), University of Liège, Campus du Sart-Tilman, Quartier Hôpital, Avenue Hippocrate, 15, B36, 4000 Liege, Belgium
*
Author to whom correspondence should be addressed.
Microorganisms 2025, 13(9), 2045; https://doi.org/10.3390/microorganisms13092045
Submission received: 15 May 2025 / Revised: 30 July 2025 / Accepted: 7 August 2025 / Published: 2 September 2025
(This article belongs to the Special Issue Editorial Board Members’ Collection Series: How Microbial Cell Factories Can Help Change the World)
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Abstract

The study of various microorganisms isolated from an Indian Ocean sponge, Scopalina hapalia ML-263, led to the selection of a promising Actinobacteria strain, Micromonospora sp. SH-82. Genomic analysis identified this strain as a new species, revealing the presence of 23 biosynthetic gene clusters (BGCs), some of which are associated with the synthesis of specialized metabolites such as polyketides deriving from polyketide synthases (PKSs). The strain was cultivated under favorable conditions for the production of bioactive molecules, resulting in the isolation and identification of seven microbial metabolites. Three of them are potentially novel, two erythronolides and one erythromycin, all characterized by a rare C10–C11 double bond. Some of these compounds also display atypical conformations, forming hemiacetals or spiroacetals. Their identification was achieved through detailed chemical analyses (NMR and ESI+-HRMS). A molecular networking approach was employed to assess the presence of potentially novel molecules in the microbial crude extract, supported by the identification of isolated molecules. Four molecules (1, 2, 3 and 5) were evaluated for their cytotoxic activities against cancer cell lines (HCT-116 and MDA-MB-231) and the immortalized retinal pigment epithelial RPE1 cells. No activity was observed in the latter, suggesting a lack of toxicity toward healthy cells. Moreover, megalomicin C1 (3), one of the isolated compounds, showed interesting antiplasmodial activity against Plasmodium falciparum 3D7, with an IC50 of 6.37 ± 2.99 µM.
Keywords: marine actinobacteria; Micromonospora; specialized metabolites; biosynthetic gene cluster; molecular network; megalomicin; erythromycin marine actinobacteria; Micromonospora; specialized metabolites; biosynthetic gene cluster; molecular network; megalomicin; erythromycin

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MDPI and ACS Style

Le Loarer, A.; Marcourt, L.; Marcellin-Gros, R.; Dufossé, L.; Ramesh, C.; Anwesh, M.; Bignon, J.; Frédérich, M.; Ledoux, A.; Ferreira Queiroz, E.; et al. New Marine Actinobacteria Strain, Micromonospora sp. SH-82: Characterization, Specialized Metabolites and Biological Activities. Microorganisms 2025, 13, 2045. https://doi.org/10.3390/microorganisms13092045

AMA Style

Le Loarer A, Marcourt L, Marcellin-Gros R, Dufossé L, Ramesh C, Anwesh M, Bignon J, Frédérich M, Ledoux A, Ferreira Queiroz E, et al. New Marine Actinobacteria Strain, Micromonospora sp. SH-82: Characterization, Specialized Metabolites and Biological Activities. Microorganisms. 2025; 13(9):2045. https://doi.org/10.3390/microorganisms13092045

Chicago/Turabian Style

Le Loarer, Alexandre, Laurence Marcourt, Rémy Marcellin-Gros, Laurent Dufossé, Chatragadda Ramesh, Maile Anwesh, Jérome Bignon, Michel Frédérich, Allison Ledoux, Emerson Ferreira Queiroz, and et al. 2025. "New Marine Actinobacteria Strain, Micromonospora sp. SH-82: Characterization, Specialized Metabolites and Biological Activities" Microorganisms 13, no. 9: 2045. https://doi.org/10.3390/microorganisms13092045

APA Style

Le Loarer, A., Marcourt, L., Marcellin-Gros, R., Dufossé, L., Ramesh, C., Anwesh, M., Bignon, J., Frédérich, M., Ledoux, A., Ferreira Queiroz, E., Wolfender, J.-L., Fouillaud, M., & Gauvin-Bialecki, A. (2025). New Marine Actinobacteria Strain, Micromonospora sp. SH-82: Characterization, Specialized Metabolites and Biological Activities. Microorganisms, 13(9), 2045. https://doi.org/10.3390/microorganisms13092045

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