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Unexpected Cross-Reaction with Honigbergiella-Like DNA in a PCR for Detection of Bovine Tritrichomonas foetus

Department of Parasitology, National Veterinary Research Institute, Partyzantow Avenue 57, 24-100 Puławy, Poland
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Academic Editor: Stefania Perrucci
Pathogens 2021, 10(4), 441; https://doi.org/10.3390/pathogens10040441
Received: 3 February 2021 / Revised: 5 April 2021 / Accepted: 6 April 2021 / Published: 8 April 2021
The prevalence of bovine Tritrichomonas foetus infection has decreased almost to zero in most European countries, such as Poland, which has been Tritrichomonas foetus-free since 1997. However, trichomonosis is a notifiable disease and there is a duty to examine samples from cattle. In this study, we present an unexpected cross-reaction with Honigbergiella-like DNA in a specimen from a bull. The bovine sample was submitted to the Department of Parasitology National Veterinary Research Institute in Pulawy (NVRI) for confirmatory testing after having been examined at the Regional Veterinary Laboratory, during a routine T. foetus diagnosis. Positive results from microscopic observation and cultures were confirmed. Noteworthily, parasites grew on Diamond’s medium only after seven days of incubation, while optimal growth of trichomonads is generally observed after two to four days for this medium. Moreover, by using PCR we obtained positive results for the presence of T. foetus. However, sequencing of the amplification product revealed 99.62% identity with Honigbergiella sp. Our data suggest that false-positive results may occur in commonly used PCR tests. Thus, unexpected results should be carefully interpreted. View Full-Text
Keywords: Tritrichomonas foetus; cattle; PCR; cross-reaction; Honigbergiella sp. Tritrichomonas foetus; cattle; PCR; cross-reaction; Honigbergiella sp.
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MDPI and ACS Style

Dąbrowska, J.; Karamon, J.; Kochanowski, M.; Sroka, J.; Cencek, T. Unexpected Cross-Reaction with Honigbergiella-Like DNA in a PCR for Detection of Bovine Tritrichomonas foetus. Pathogens 2021, 10, 441. https://doi.org/10.3390/pathogens10040441

AMA Style

Dąbrowska J, Karamon J, Kochanowski M, Sroka J, Cencek T. Unexpected Cross-Reaction with Honigbergiella-Like DNA in a PCR for Detection of Bovine Tritrichomonas foetus. Pathogens. 2021; 10(4):441. https://doi.org/10.3390/pathogens10040441

Chicago/Turabian Style

Dąbrowska, Joanna; Karamon, Jacek; Kochanowski, Maciej; Sroka, Jacek; Cencek, Tomasz. 2021. "Unexpected Cross-Reaction with Honigbergiella-Like DNA in a PCR for Detection of Bovine Tritrichomonas foetus" Pathogens 10, no. 4: 441. https://doi.org/10.3390/pathogens10040441

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