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Open AccessArticle

Unexpected Diversity of Wolbachia Associated with Bactrocera dorsalis (Diptera: Tephritidae) in Africa

1
International Centre of Insect Physiology and Ecology (icipe), Kasarani, Nairobi 00100, Kenya
2
Unit for Environmental Sciences and Management, North-West University, Potchefstroom 2520, South Africa
3
MRC-University of Glasgow Centre for Virus Research, Henry Wellcome Building, Glasgow G61 1QH, UK
*
Author to whom correspondence should be addressed.
Insects 2019, 10(6), 155; https://doi.org/10.3390/insects10060155
Received: 8 February 2019 / Revised: 17 May 2019 / Accepted: 20 May 2019 / Published: 31 May 2019
Bactrocera dorsalis (Hendel) is an important pest of fruit-bearing plants in many countries worldwide. In Africa, this pest has spread rapidly and has become widely established since the first invasion report in 2003. Wolbachia is a vertically transmitted endosymbiont that can significantly influence aspects of the biology and, in particular, the reproduction of its host. In this study, we screened B. dorsalis specimens collected from several locations in Africa between 2005 and 2017 for Wolbachia using a PCR-based assay to target the Wolbachia surface protein wsp. Of the 357 individuals tested, 10 were positive for Wolbachia using the wsp assay. We identified four strains of Wolbachia infecting two B. dorsalis mitochondrial haplotypes. We found no strict association between the infecting strain and host haplotype, with one strain being present in two different host haplotypes. All the detected strains belonged to Super Group B Wolbachia and did not match any strains reported previously in B. dorsalis in Asia. These findings indicate that diverse Wolbachia infections are present in invasive populations of B. dorsalis. View Full-Text
Keywords: Wolbachia; Bactrocera dorsalis; oriental fruit fly; Wolbachia surface protein; mitochondrial COI haplotype Wolbachia; Bactrocera dorsalis; oriental fruit fly; Wolbachia surface protein; mitochondrial COI haplotype
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Gichuhi, J.; Khamis, F.M.; Van den Berg, J.; Ekesi, S.; Herren, J.K. Unexpected Diversity of Wolbachia Associated with Bactrocera dorsalis (Diptera: Tephritidae) in Africa. Insects 2019, 10, 155.

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