Molecular Cloning and Expression Analysis of Three Suppressors of Cytokine Signaling Genes (SOCS5, SOCS6, SOCS7) in the Mealworm Beetle Tenebrio molitor

Patnaik, Bharat Bhusan; Kim, Bo Bae; Jo, Yong Hun; Bang, In Seok

doi:10.3390/insects10030076

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Molecular Cloning and Expression Analysis of Three Suppressors of Cytokine Signaling Genes (SOCS5, SOCS6, SOCS7) in the Mealworm Beetle Tenebrio molitor

Bharat Bhusan Patnaik

^1,2

Bo Bae Kim

¹,

Yong Hun Jo

^1,* and

In Seok Bang

^3,*

Division of Plant Biotechnology, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea

School of Biotech Sciences, Trident Academy of Creative Technology (TACT), Chandrasekharpur, Bhubaneswar, Odisha 751024, India

Department of Biological Science and the Research Institute for Basic Sciences, Hoseo University, Asan 31499, Korea

Authors to whom correspondence should be addressed.

Insects 2019, 10(3), 76; https://doi.org/10.3390/insects10030076

Received: 24 January 2019 / Revised: 6 March 2019 / Accepted: 9 March 2019 / Published: 16 March 2019

(This article belongs to the Special Issue Insect Immunity and Pathology)

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Abstract

Suppressors of cytokine signaling (SOCS) influence cytokine and growth factor signaling by negatively regulating the Janus kinase (JAK)-signal transducers and activators of transcription (STAT) pathway to maintain homeostasis during immune responses. However, functional characterization of SOCS family members in invertebrates is limited. Here, we identified and evaluated three SOCS genes (type I sub-family) in the mealworm beetle Tenebrio molitor. The full-length open reading frames (ORFs) of TmSOCS5, TmSOCS6, and TmSOCS7 comprised of 1389, 897, and 1458 nucleotides, encoding polypeptides of 462, 297, and 485 amino acids, respectively. The SH2 and SOCS box domains of the TmSOCS C-terminal region were highly conserved. Phylogenetic analysis revealed that these SOCS genes were clustered within the type I subfamily that exhibits the highest amino acid identity with Tribolium castaneum SOCS genes. Contrary to TmSOCS7 expression, the expression levels of TmSOCS5 and TmSOCS6 were lower in the larval, pupal, and adult stages. In larvae and adults, the expression levels of TmSOCS5 and TmSOCS6 were highest in the hemocytes and ovaries, respectively. SOCS transcripts were also highly upregulated in the hemocytes of T. molitor larvae within 3–6 h post-infection with the fungus Candida albicans. Collectively, these results provide valuable information regarding the involvement of TmSOCS type-I subfamily in the host immune response of insects. View Full-Text

Keywords: Tenebrio molitor; suppressor of cytokine signaling; insect immunity; gene expression

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Patnaik, B.B.; Kim, B.B.; Jo, Y.H.; Bang, I.S. Molecular Cloning and Expression Analysis of Three Suppressors of Cytokine Signaling Genes (SOCS5, SOCS6, SOCS7) in the Mealworm Beetle Tenebrio molitor. Insects 2019, 10, 76. https://doi.org/10.3390/insects10030076

AMA Style

Patnaik BB, Kim BB, Jo YH, Bang IS. Molecular Cloning and Expression Analysis of Three Suppressors of Cytokine Signaling Genes (SOCS5, SOCS6, SOCS7) in the Mealworm Beetle Tenebrio molitor. Insects. 2019; 10(3):76. https://doi.org/10.3390/insects10030076

Chicago/Turabian Style

Patnaik, Bharat B., Bo B. Kim, Yong H. Jo, and In S. Bang. 2019. "Molecular Cloning and Expression Analysis of Three Suppressors of Cytokine Signaling Genes (SOCS5, SOCS6, SOCS7) in the Mealworm Beetle Tenebrio molitor" Insects 10, no. 3: 76. https://doi.org/10.3390/insects10030076

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Molecular Cloning and Expression Analysis of Three Suppressors of Cytokine Signaling Genes (SOCS5, SOCS6, SOCS7) in the Mealworm Beetle Tenebrio molitor

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