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Article

Development of Immunochromatographic Test Kit for Rapid Detection of Specific IgG4 Antibody in Whole-Blood Samples for Diagnosis of Human Gnathostomiasis

1
Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161, Thailand
2
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
3
Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand
4
Department of Parasitology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan
5
Faculty of Medicine, Mahasarakham University, Maha Sarakham 44000, Thailand
6
Clinical Immunology Unit, Srinagarind Hospital, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
*
Author to whom correspondence should be addressed.
Academic Editors: Chao-Min Cheng, Majid Ebrahimi Warkiani and Meysam Rezaei
Diagnostics 2021, 11(5), 862; https://doi.org/10.3390/diagnostics11050862
Received: 10 April 2021 / Revised: 4 May 2021 / Accepted: 8 May 2021 / Published: 11 May 2021
(This article belongs to the Special Issue Advanced Diagnostic Kit Development for Laboratory Diagnosis)
Human gnathostomiasis is a harmful food-borne zoonosis caused by roundworms of the genus Gnathostoma. The parasite can occasionally migrate to the central nervous system, causing life-threatening disease and death. Here, we report a new point-of-care (POC) test kit, the gnathostomiasis blood immunochromatographic test (GB-ICT) kit. The kit is based on recombinant Gnathostoma spinigerum antigen and detects specific IgG4 antibody in whole-blood samples (WBSs). The GB-ICT kit showed potentially high diagnostic values with simulated WBSs (n = 248), which were obtained by spiking patients’ sera with red blood cells. The accuracy, sensitivity, specificity, and positive and negative predictive values were 95.2%, 100%, 93.8%, 81.5%, and 100%, respectively. Ten WBSs from clinically suspected gnathostomiasis patients were all positive according to the GB-ICT kit, while 10 WBSs from healthy volunteers were negative. The GB-ICT kit is a simple and convenient POC testing tool using finger-prick blood samples: venous blood sampling and serum separation processes are not required. The GB-ICT kit can support clinical diagnosis in remote areas and field settings without sophisticated equipment facilities. View Full-Text
Keywords: human gnathostomiasis; immunochromatographic test kit; point-of-care test; whole-blood sample; serodiagnosis; IgG4 antibody human gnathostomiasis; immunochromatographic test kit; point-of-care test; whole-blood sample; serodiagnosis; IgG4 antibody
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MDPI and ACS Style

Janwan, P.; Intapan, P.M.; Sadaow, L.; Rodpai, R.; Yamasaki, H.; Boonroumkaew, P.; Sanpool, O.; Thanchomnang, T.; Sadee, P.; Maleewong, W. Development of Immunochromatographic Test Kit for Rapid Detection of Specific IgG4 Antibody in Whole-Blood Samples for Diagnosis of Human Gnathostomiasis. Diagnostics 2021, 11, 862. https://doi.org/10.3390/diagnostics11050862

AMA Style

Janwan P, Intapan PM, Sadaow L, Rodpai R, Yamasaki H, Boonroumkaew P, Sanpool O, Thanchomnang T, Sadee P, Maleewong W. Development of Immunochromatographic Test Kit for Rapid Detection of Specific IgG4 Antibody in Whole-Blood Samples for Diagnosis of Human Gnathostomiasis. Diagnostics. 2021; 11(5):862. https://doi.org/10.3390/diagnostics11050862

Chicago/Turabian Style

Janwan, Penchom; Intapan, Pewpan M.; Sadaow, Lakkhana; Rodpai, Rutchanee; Yamasaki, Hiroshi; Boonroumkaew, Patcharaporn; Sanpool, Oranuch; Thanchomnang, Tongjit; Sadee, Phuangphaka; Maleewong, Wanchai. 2021. "Development of Immunochromatographic Test Kit for Rapid Detection of Specific IgG4 Antibody in Whole-Blood Samples for Diagnosis of Human Gnathostomiasis" Diagnostics 11, no. 5: 862. https://doi.org/10.3390/diagnostics11050862

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