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Communication

Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis

1
Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80756, Taiwan
2
Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung 80756, Taiwan
3
College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
*
Author to whom correspondence should be addressed.
Diagnostics 2020, 10(5), 333; https://doi.org/10.3390/diagnostics10050333
Received: 22 April 2020 / Revised: 18 May 2020 / Accepted: 19 May 2020 / Published: 21 May 2020
Coronavirus disease 2019 (COVID-19), the current uncontrolled outbreak of infectious disease, has caused significant challenges throughout the world. A reliable rapid diagnostic test for COVID-19 is demanded worldwide. The real-time reverse transcriptase polymerase chain was one of the most quickly established methods in the novel viral pandemic and was considered as the gold standard for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this report, we illustrate our experience of applying a protocol from the Taiwan CDC and achieving assay optimization in the immediate circumstances to meet the urgent medical and public health needs. View Full-Text
Keywords: coronavirus disease 2019 (COVID-19); severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); molecular diagnostics; real-time reverse transcriptase polymerase chain (RT-PCR) coronavirus disease 2019 (COVID-19); severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); molecular diagnostics; real-time reverse transcriptase polymerase chain (RT-PCR)
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MDPI and ACS Style

Chen, C.-J.; Hsieh, L.-L.; Lin, S.-K.; Wang, C.-F.; Huang, Y.-H.; Lin, S.-Y.; Lu, P.-L. Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis. Diagnostics 2020, 10, 333. https://doi.org/10.3390/diagnostics10050333

AMA Style

Chen C-J, Hsieh L-L, Lin S-K, Wang C-F, Huang Y-H, Lin S-Y, Lu P-L. Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis. Diagnostics. 2020; 10(5):333. https://doi.org/10.3390/diagnostics10050333

Chicago/Turabian Style

Chen, Chao-Ju, Li-Ling Hsieh, Shu-Kai Lin, Chu-Feng Wang, Yi-Hui Huang, Shang-Yi Lin, and Po-Liang Lu. 2020. "Optimization of the CDC Protocol of Molecular Diagnosis of COVID-19 for Timely Diagnosis" Diagnostics 10, no. 5: 333. https://doi.org/10.3390/diagnostics10050333

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