The Effect of Tobacco Smoking and Smoking Cessation on Urinal miRNAs in a Pilot Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Population
2.2. Sample Collection and Proceeding
2.3. miRNA Microarray Processing and qPCR
2.4. Data Analysis and Statistics
3. Results
3.1. Urine Cotinine
3.2. Urine miRNA Pattern
3.3. The Urine miRNA Profiles of Current/Ex-Smokers Versus Never Smokers in a Baseline Design Study
3.4. The Effect of Short-Term and Long-Term Abstinence in the Same Patient
3.5. Validation with qPCR
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Analysed Group (Total Number of Samples, n) | Age (Years) Mean (Min.-Max.) | Sex (F:M) | Pack-Year Mean * (Min.-Max.) | Urine Cotinine (ng/mL) Mean (SD, Min.-Max.) | Exhaled CO (ppm) Mean (SD, Min.-Max.) | Urine Creatinine (mmol/l) (SD, Min.-Max.] |
Never smokers (6) | 54 (39–83) | 3:3 | 0 | <10 | 0 | 10.81 (4.88, 2.34–15.66) |
Current smokers (10) | 48 (27–73) | 2:8 | 29 (7–83) | 6140 (3184, 1325–11,712) | 12.4 (7.183, 1.000–25.000) | 12.86 (7.50, 2.69–29.89) |
Short-term successful ex-smokers (10) | 48 (27–73) | 2:8 | 29 (7–83) | 684 (1346, 10–4359) | 0.400 (1.265, 0.000–4.000) | 11.96 (7.99, 2.22–25.53) |
Long-term successful ex-smokers (7) | 46 (27–67) | 2:5 | 33 (7–83) | 20 (16, 10–54) | 0.286 (0.488, 0.000–1.000) | 12.88 (5.11, 3.48–18.65) |
Analysed Group (Total Number of Samples, n) | Age (Years) Mean (Min.-Max.) | Sex (F:M) | Pack-Year * Mean (Min.-Max.) | Urine Cotinine (ng/mL) Mean (SD, Min.-Max.) | Exhaled CO (ppm) Mean (SD, Min.-Max.) | Urine Creatinine (mmol/l) (SD, Min.-Max.) |
Never smokers (18) | 50 (38–83) | 9:9 | 0 | <10 | 0 | 10.81 (4.88, 2.34–15.66) |
Current smokers (25) | 47 (25–69) | 10:15 | 35 (5–170) | 4851 (2689, 736–11,712) | 8.857 (6.139, 1.000–25.000) | 10.71 (7.95, 2.05–29.89) |
Short-term successful ex-smokers (25) | 47 (25–69) | 10:15 | 35 (5–170) | 546 (875, 12–3285) | 0.286 (0.810, 0.000–4.000) | 9.69 (5.74, 2.22–22.54) |
Long-term successful ex-smokers (25) | 47 (25–69) | 10:15 | 35 (5–170) | 24 (15, 14–54) | 0.107 (0.315, 0.000–1.000) | 12.87 (4.36, 3.48– 8.65) |
Vs. Never Smokers | Mature miRNA | logFC | p Value | adj. P. Val |
---|---|---|---|---|
current smokers | hsa-miR-3613-5p | −4.86 | 3.30e-10 | 8.51e-07 |
hsa-miR-3921 | −4.14 | 5.43e-07 | 6.99e-04 | |
hsa-miR-5094 | −1.09 | 1.12e-06 | 9.65e-04 | |
hsa-miR-337-3p | −2.38 | 2.84e-06 | 1.83e-03 | |
hsa-miR-3620-5p | 1.64 | 7.19e-05 | 3.71e-02 | |
short-term ex-smokers | hsa-miR-3613-5p | −5.77 | 5.58e-12 | 1.44e-08 |
hsa-miR-337-3p | −2.70 | 3.11e-07 | 3.04e-04 | |
hsa-miR-3921 | −4.24 | 3.54e-07 | 3.04e-04 | |
hsa-miR-5094 | −1.13 | 6.26e-07 | 4.04e-04 | |
hsa-miR-7159-5p | −0.44 | 1.37e-05 | 7.05e-03 | |
hsa-miR-1298-3p | −1.70 | 6.77e-05 | 2.91e-02 | |
hsa-miR-5004-5p | −0.55 | 1.37e-04 | 0.05 | |
hsa-miR-6501-3p | −0.40 | 1.64e-04 | 0.05 | |
hsa-miR-3620-5p | 1.51 | 2.05e-04 | 0.06 | |
long-term ex-smokers | hsa-miR-3613-5p | −5.33 | 2.23e-10 | 5.75e-07 |
hsa-miR-5094 | −0.90 | 6.45e-05 | 0.08 |
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Navratilova, Z.; Losse, S.; Petrova, P.; Sikorova, K.; Chabronova, A.; Petrek, M. The Effect of Tobacco Smoking and Smoking Cessation on Urinal miRNAs in a Pilot Study. Life 2020, 10, 191. https://doi.org/10.3390/life10090191
Navratilova Z, Losse S, Petrova P, Sikorova K, Chabronova A, Petrek M. The Effect of Tobacco Smoking and Smoking Cessation on Urinal miRNAs in a Pilot Study. Life. 2020; 10(9):191. https://doi.org/10.3390/life10090191
Chicago/Turabian StyleNavratilova, Zdenka, Stanislav Losse, Pavla Petrova, Katerina Sikorova, Alzbeta Chabronova, and Martin Petrek. 2020. "The Effect of Tobacco Smoking and Smoking Cessation on Urinal miRNAs in a Pilot Study" Life 10, no. 9: 191. https://doi.org/10.3390/life10090191