TATA Binding Protein (TBP) Promoter Drives Ubiquitous Expression of Marker Transgene in the Adult Sea Anemone Nematostella vectensis

Admoni, Yael; Kozlovski, Itamar; Lewandowska, Magda; Moran, Yehu

doi:10.3390/genes11091081

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TATA Binding Protein (TBP) Promoter Drives Ubiquitous Expression of Marker Transgene in the Adult Sea Anemone Nematostella vectensis

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Department of Ecology, Evolution and Behavior, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel

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These authors contributed equally to this work.

Genes 2020, 11(9), 1081; https://doi.org/10.3390/genes11091081

Received: 14 August 2020 / Revised: 13 September 2020 / Accepted: 14 September 2020 / Published: 16 September 2020

(This article belongs to the Section Animal Genetics and Genomics)

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Abstract

Nematostella vectensis has emerged as one as the most established models of the phylum Cnidaria (sea anemones, corals, hydroids and jellyfish) for studying animal evolution. The availability of a reference genome and the relative ease of culturing and genetically manipulating this organism make it an attractive model for addressing questions regarding the evolution of venom, development, regeneration and other interesting understudied questions. We and others have previously reported the use of tissue-specific promoters for investigating the function of a tissue or a cell type of interest in vivo. However, to our knowledge, genetic regulators at the whole organism level have not been reported yet. Here we report the identification and utilization of a ubiquitous promoter to drive a wide and robust expression of the fluorescent protein mCherry. We generated animals containing a TATA binding protein (TBP) promoter upstream of the mCherry gene. Flow cytometry and fluorescent microscopy revealed expression of mCherry in diverse cell types, accounting for more than 90% of adult animal cells. Furthermore, we detected a stable mCherry expression at different life stages and throughout generations. This tool will expand the existing experimental toolbox to facilitate genetic engineering and functional studies at the whole organism level. View Full-Text

Keywords: Nematostella vectensis; transgenic animal; ubiquitous promoter; gene expression

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Admoni, Y.; Kozlovski, I.; Lewandowska, M.; Moran, Y. TATA Binding Protein (TBP) Promoter Drives Ubiquitous Expression of Marker Transgene in the Adult Sea Anemone Nematostella vectensis. Genes 2020, 11, 1081. https://doi.org/10.3390/genes11091081

AMA Style

Admoni Y, Kozlovski I, Lewandowska M, Moran Y. TATA Binding Protein (TBP) Promoter Drives Ubiquitous Expression of Marker Transgene in the Adult Sea Anemone Nematostella vectensis. Genes. 2020; 11(9):1081. https://doi.org/10.3390/genes11091081

Chicago/Turabian Style

Admoni, Yael, Itamar Kozlovski, Magda Lewandowska, and Yehu Moran. 2020. "TATA Binding Protein (TBP) Promoter Drives Ubiquitous Expression of Marker Transgene in the Adult Sea Anemone Nematostella vectensis" Genes 11, no. 9: 1081. https://doi.org/10.3390/genes11091081

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TATA Binding Protein (TBP) Promoter Drives Ubiquitous Expression of Marker Transgene in the Adult Sea Anemone Nematostella vectensis

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