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IL-3-Induced Immediate Expression of c-fos and c-jun Is Modulated by the IKK2-JNK Axis

Department of Biochemistry and Genome Biology, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Aomori, Japan
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Academic Editor: Stephen Yarwood
Cells 2022, 11(9), 1451; https://doi.org/10.3390/cells11091451
Received: 12 April 2022 / Accepted: 21 April 2022 / Published: 25 April 2022
(This article belongs to the Section Cell Signaling)
Interleukin (IL)-3 is a pleiotropic cytokine that regulates the survival, proliferation, and differentiation of hematopoietic cells. The binding of IL-3 to its receptor activates intracellular signaling, inducing transcription of immediate early genes (IEGs) such as c-fos, c-jun, and c-myc; however, transcriptional regulation under IL-3 signaling is not fully understood. This study assessed the role of the inhibitor of nuclear factor-κB kinases (IKKs) in inducing IL-3-mediated expression of IEGs. We show that IKK1 and IKK2 are required for the IL-3-induced immediate expression of c-fos and c-jun in murine hematopoietic Ba/F3 cells. Although IKK2 is well-known for its pivotal role as a regulator of the canonical nuclear factor-κB (NF-κB) pathway, activation of IKKs did not induce the nuclear translocation of the NF-κB transcription factor. We further revealed the important role of IKK2 in the activation of c-Jun N-terminal kinase (JNK), which mediates the IL-3-induced expression of c-fos and c-jun. These findings indicate that the IKK2-JNK axis modulates the IL-3-induced expression of IEGs in a canonical NF-κB-independent manner. View Full-Text
Keywords: IL-3; IKK; immediate early gene; JNK IL-3; IKK; immediate early gene; JNK
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MDPI and ACS Style

Fujita, H.; Fujita, T.; Fujii, H. IL-3-Induced Immediate Expression of c-fos and c-jun Is Modulated by the IKK2-JNK Axis. Cells 2022, 11, 1451. https://doi.org/10.3390/cells11091451

AMA Style

Fujita H, Fujita T, Fujii H. IL-3-Induced Immediate Expression of c-fos and c-jun Is Modulated by the IKK2-JNK Axis. Cells. 2022; 11(9):1451. https://doi.org/10.3390/cells11091451

Chicago/Turabian Style

Fujita, Hirotaka, Toshitsugu Fujita, and Hodaka Fujii. 2022. "IL-3-Induced Immediate Expression of c-fos and c-jun Is Modulated by the IKK2-JNK Axis" Cells 11, no. 9: 1451. https://doi.org/10.3390/cells11091451

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