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10.3390/cells11132027
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Article

CCL22-Polarized TAMs to M2a Macrophages in Cervical Cancer In Vitro Model

by
Qun Wang
1,
Kritika Sudan
2,
Elisa Schmoeckel
3,
Bernd Peter Kost
1,
Christina Kuhn
1,4,
Aurelia Vattai
1,
Theresa Vilsmaier
1,
Sven Mahner
1,
Udo Jeschke
1,4,* and
Helene Hildegard Heidegger
1
1
Department of Obstetrics and Gynecology, University Hospital, LMU Munich, 80377 Munich, Germany
2
Department of Cardiology, University Hospital Munich, LMU Munich, 81377 Munich, Germany
3
Department of Pathology, LMU Munich, 80377 Munich, Germany
4
Department of Obstetrics and Gynecology, University Hospital Augsburg, 86156 Augsburg, Germany
*
Author to whom correspondence should be addressed.
Cells 2022, 11(13), 2027; https://doi.org/10.3390/cells11132027
Submission received: 2 May 2022 / Revised: 20 June 2022 / Accepted: 23 June 2022 / Published: 25 June 2022
(This article belongs to the Special Issue Tumor Immune Microenvironment for Effective Therapy)
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Abstract

Macrophages are dynamic cells susceptible to the local microenvironment which includes tumor-associated macrophages (TAMs) in cancers. TAMs are a collection of heterogeneous macrophages, including M1 and M2 subtypes, shaped by various activation modes and labeled with various markers in different tumors. CCL22+-infiltrating cells are thought to be significantly associated with the prognosis of cervical cancer patients. Moreover, CCL22 is an established marker of M2a macrophages. Although the phenotypic identification of M1 and M2 macrophages is well established in mice and human macrophages cultured in a medium with fetal calf serum (FCS), fewer studies have focused on M2 subtypes. In addition, the question of whether CCL22 affects polarization of M2a macrophages remains unanswered. This study constructed a co-culture system to shape TAMs in vitro. We found that CCL22 was mainly secreted by TAMs but not cervical cancer cell lines. Human peripheral blood monocytes were differentiated into uncommitted macrophages (M0) and then polarized to M1, M2a, M2b, and M2c macrophages using LPS plus IFNr, IL-4, LPS plus IL1β, and IL-10, respectively. Using flowcytometry, we found CD80++ was the marker of M1 and M2b, CD206++ was the marker of M2a, and CD163++ was the marker of M2c, compared with M0 macrophages. By regulating CCL22, we found that the mean fluorescence intensity (MFI) of CD206 in TAMs was significantly affected compared to the control group. Therefore, CCL22 could polarize TAMs of cervical cancer toward M2a macrophages. In conclusion, our study revealed that CCL22 could be a therapeutic target for cervical cancer, which might be because of its role in regulating macrophage polarization.
Keywords: cervical cancer; TAMs; CCL22; M2a macrophages cervical cancer; TAMs; CCL22; M2a macrophages

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MDPI and ACS Style

Wang, Q.; Sudan, K.; Schmoeckel, E.; Kost, B.P.; Kuhn, C.; Vattai, A.; Vilsmaier, T.; Mahner, S.; Jeschke, U.; Heidegger, H.H. CCL22-Polarized TAMs to M2a Macrophages in Cervical Cancer In Vitro Model. Cells 2022, 11, 2027. https://doi.org/10.3390/cells11132027

AMA Style

Wang Q, Sudan K, Schmoeckel E, Kost BP, Kuhn C, Vattai A, Vilsmaier T, Mahner S, Jeschke U, Heidegger HH. CCL22-Polarized TAMs to M2a Macrophages in Cervical Cancer In Vitro Model. Cells. 2022; 11(13):2027. https://doi.org/10.3390/cells11132027

Chicago/Turabian Style

Wang, Qun, Kritika Sudan, Elisa Schmoeckel, Bernd Peter Kost, Christina Kuhn, Aurelia Vattai, Theresa Vilsmaier, Sven Mahner, Udo Jeschke, and Helene Hildegard Heidegger. 2022. "CCL22-Polarized TAMs to M2a Macrophages in Cervical Cancer In Vitro Model" Cells 11, no. 13: 2027. https://doi.org/10.3390/cells11132027

APA Style

Wang, Q., Sudan, K., Schmoeckel, E., Kost, B. P., Kuhn, C., Vattai, A., Vilsmaier, T., Mahner, S., Jeschke, U., & Heidegger, H. H. (2022). CCL22-Polarized TAMs to M2a Macrophages in Cervical Cancer In Vitro Model. Cells, 11(13), 2027. https://doi.org/10.3390/cells11132027

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