Evaluation of the International Society for Cutaneous Lymphoma Algorithm for the Diagnosis of Early Mycosis Fungoides
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients and Samples
2.2. IHC Staining
2.3. DNA Extraction and TCR Gene Rearrangement Analysis
2.4. Statistical Analysis
3. Results
3.1. Clinicopathologic Features of Early MF
3.2. Determinative Immunopathologic Markers of Early MF
3.3. Molecular Features of Early MF
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Early MF (n = 38) | Non-MF (n = 22) | p-Value | |
---|---|---|---|
Sex (male:female) | 22:16 | 10:12 | 0.4254 |
Age (years, mean ± SD) | 43.13 ± 2.858 | 51.91 ± 3.896 | 0.0717 |
Total ISCL score (mean ± SD) | 4.868 ± 0.1261 | 2.0 ± 0.1861 | <0.0001 |
Clinical points | <0.0001 | ||
0 1 2 | 1 7 30 | 9 8 5 | |
Histopathologic points | 0.0500 | ||
0 1 2 | 3 15 20 | 5 12 5 | |
Molecular/biologic points | <0.0001 | ||
0 1 | 2 34 | 16 4 | |
Immunopathologic points | <0.0001 | ||
0 1 | 11 25 | 20 0 |
Early MF (n = 38) | Non-MF (n = 22) | p-Value | |
---|---|---|---|
IHC (%, ± SEM) CD2 CD3 CD5 CD7 | |||
84.08 ± 3.155 91.84 ± 1.722 81.18 ± 3.438 17.45 ± 2.648 | 92.27 ± 1.174 93.41 ± 0.764 92.50 ± 1.175 42.95 ± 4.763 | 0.0590 0.5065 0.0174 <0.0001 | |
Epidermal discordance CD2 CD3 CD5 CD7 | 0 0 2 15 | 0 0 0 0 | >0.9999 >0.9999 0.5402 0.0010 |
IHC | AUC | Muller’s Value | SE | 95% Confidence Interval | p-Value | Cut-Off (%) | Sensitivity (%) | Specificity (%) | |
---|---|---|---|---|---|---|---|---|---|
Lower | Upper | ||||||||
CD2 CD3 CD5 CD7 | 0.669 0.531 0.718 0.837 | Poor Fail Fair Good | 0.071 0.077 0.067 0.050 | 0.529 0.379 0.587 0.739 | 0.808 0.682 0.849 0.934 | 0.031 0.696 0.005 0.000 | 92.5 92.5 92.5 22.5 | 60.5 28.9 65.8 73.7 | 68.2 77.3 72.7 77.3 |
Early MF (n = 38) | Non-MF (n = 22) | p-Value | |
---|---|---|---|
TCR gene rearrangement TCR-γ TCR-δ TCR-β | <0.0001 | ||
33 (86.8%) 6 (15.8%) 16 (42.1%) | 4 (18.2%) 3 (13.6%) 0 (0.0%) | <0.0001 >0.9999 0.0002 |
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Ryu, H.-J.; Kim, S.-I.; Jang, H.-O.; Kim, S.-H.; Oh, S.-H.; Park, S.; Kim, S.-K. Evaluation of the International Society for Cutaneous Lymphoma Algorithm for the Diagnosis of Early Mycosis Fungoides. Cells 2021, 10, 2758. https://doi.org/10.3390/cells10102758
Ryu H-J, Kim S-I, Jang H-O, Kim S-H, Oh S-H, Park S, Kim S-K. Evaluation of the International Society for Cutaneous Lymphoma Algorithm for the Diagnosis of Early Mycosis Fungoides. Cells. 2021; 10(10):2758. https://doi.org/10.3390/cells10102758
Chicago/Turabian StyleRyu, Hyang-Joo, Sun-Il Kim, Hyung-Ook Jang, Se-Hoon Kim, Sang-Ho Oh, Sujin Park, and Sang-Kyum Kim. 2021. "Evaluation of the International Society for Cutaneous Lymphoma Algorithm for the Diagnosis of Early Mycosis Fungoides" Cells 10, no. 10: 2758. https://doi.org/10.3390/cells10102758