Error in Figure
In the original publication [1], there was a mistake in Figure 2b as published. The wrong image was used for the Huh-7 control (2b tubulin). The corrected Figure 2b and appears below. The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.
Figure 2.
Effects of 5-Aza on cell cytoskeleton. (a,b) Immunostaining of β-actin and β-tubulin in JHH-6 and HuH-7 cells, respectively. Images were acquired with a Leica DM 2000 microscope. Red = β-actin; green = β-tubulin; blue = DAPI. Magnification 40X (bar = 100 μm). (c) Ratio of polymerized/cytosolic α-tubulin in 5-Aza treated vs. non-treated cells (NTC); data are expressed as mean ± SEM; JHH-6 NTC vs. JHH-6/5-Aza, § p = 0.04; HuH-7 NTC vs. HuH-7/5-Aza, $ p = 0.045, n = 3. (d,e) Quantification of the number of altered nuclei shape in 5-Aza-treated vs. non-treated cells. Data, expressed as mean ± SEM, are reported as percentage normalized to the average of non-treated cells (NTC). Abnormal nuclei in JHH6/NTC vs. JHH6/0,8-6 μM, * p < 0.0001 n = 60; HuH7/NTC vs. HuH7/6-30 μM, # p < 0.001 n = 60.
Reference
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