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Micromachines, Volume 15, Issue 5 (May 2024) – 65 articles

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12 pages, 10693 KiB  
Article
Capillary Force-Driven Quantitative Plasma Separation Method for Application of Whole Blood Detection Microfluidic Chip
by Xiaohua Fang, Cuimin Sun, Peng Dai, Zhaokun Xian, Wenyun Su, Chaowen Zheng, Dong Xing, Xiaotian Xu and Hui You
Micromachines 2024, 15(5), 619; https://doi.org/10.3390/mi15050619 - 01 May 2024
Viewed by 188
Abstract
Separating plasma or serum from blood is essential for precise testing. However, extracting precise plasma quantities outside the laboratory poses challenges. A recent study has introduced a capillary force-driven membrane filtration technique to accurately separate small plasma volumes. This method efficiently isolates 100–200 [...] Read more.
Separating plasma or serum from blood is essential for precise testing. However, extracting precise plasma quantities outside the laboratory poses challenges. A recent study has introduced a capillary force-driven membrane filtration technique to accurately separate small plasma volumes. This method efficiently isolates 100–200 μL of pure human whole blood with a 48% hematocrit, resulting in 5–30 μL of plasma with less than a 10% margin of error. The entire process is completed within 20 min, offering a simple and cost-effective approach to blood separation. This study has successfully addressed the bottleneck in self-service POCT, ensuring testing accuracy. This innovative method shows promise for clinical diagnostics and point-of-care testing. Full article
(This article belongs to the Special Issue Recent Advances in Lab-on-a-Chip and Their Biomedical Applications)
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25 pages, 11771 KiB  
Article
An Experimental Investigation of R600a Condensation in a Multiport Microchannel
by Burak Çoban and Lütfullah Kuddusi
Micromachines 2024, 15(5), 618; https://doi.org/10.3390/mi15050618 - 01 May 2024
Viewed by 214
Abstract
This study aims to provide condensation heat transfer coefficients of R600a (isobutane) refrigerant under mass fluxes between 50 and 98 kg/m2·s at saturation temperatures of 35 °C, 40 °C and 45 °C. Additionally, experiments are conducted with varying inlet vapour quality [...] Read more.
This study aims to provide condensation heat transfer coefficients of R600a (isobutane) refrigerant under mass fluxes between 50 and 98 kg/m2·s at saturation temperatures of 35 °C, 40 °C and 45 °C. Additionally, experiments are conducted with varying inlet vapour quality to understand its effect on the condensation heat transfer measurement. An aluminium multiport microchannel with a hydraulic diameter () of 0.399 mm is used, where a plexiglass cover is mounted on the top of the microchannels to observe the flow conditions. A 1D heat transfer through the aluminium block is assumed, and heat flux through the refrigerant to the coolant is measured to obtain condensation heat transfer coefficients of R600a. The results showed that decreasing saturation temperature and increasing vapour quality increase the condensation heat transfer coefficient. Increasing refrigerant mass flux increases the heat transfer coefficient up to a specific mass flux. It is observed that the effect of inlet vapour quality becomes significant as introduced quality decreases due to increasing fluctuation. Full article
11 pages, 1191 KiB  
Article
Temperature-Decoupled Single-Crystal MgO Fiber-Optic Fabry–Perot Vibration Sensor Based on MEMS Technology for Harsh Environments
by Chengxin Su, Pinggang Jia, Aihao Zhao, Jiacheng Tu, Jia Liu, Qianyu Ren and Jijun Xiong
Micromachines 2024, 15(5), 616; https://doi.org/10.3390/mi15050616 - 01 May 2024
Viewed by 199
Abstract
A high-temperature-resistance single-crystal magnesium oxide (MgO) extrinsic Fabry–Perot (FP) interferometer (EFPI) fiber-optic vibration sensor is proposed and experimentally demonstrated at 1000 °C. Due to the excellent thermal properties (melting point > 2800 °C) and optical properties (transmittance ≥ 90%), MgO is chosen as [...] Read more.
A high-temperature-resistance single-crystal magnesium oxide (MgO) extrinsic Fabry–Perot (FP) interferometer (EFPI) fiber-optic vibration sensor is proposed and experimentally demonstrated at 1000 °C. Due to the excellent thermal properties (melting point > 2800 °C) and optical properties (transmittance ≥ 90%), MgO is chosen as the ideal material to be placed in the high-temperature testing area. The combination of wet chemical etching and direct bonding is used to construct an all-MgO sensor head, which is favorable to reduce the temperature gradient inside the sensor structure and avoid sensor failure. A temperature decoupling method is proposed to eliminate the cross-sensitivity between temperature and vibration, improving the accuracy of vibration detection. The experimental results show that the sensor is stable at 20−1000 °C and 2−20 g, with a sensitivity of 0.0073 rad (20 °C). The maximum nonlinearity error of the vibration sensor measurement after temperature decoupling is 1.17%. The sensor with a high temperature resistance and outstanding dynamic performance has the potential for applications in testing aero-engines and gas turbine engines. Full article
(This article belongs to the Special Issue Micro/Nano Sensors: Fabrication and Applications)
16 pages, 1438 KiB  
Article
DAM SRAM CORE: An Efficient High-Speed and Low-Power CIM SRAM CORE Design for Feature Extraction Convolutional Layers in Binary Neural Networks
by Ruiyong Zhao, Zhenghui Gong, Yulan Liu and Jing Chen
Micromachines 2024, 15(5), 617; https://doi.org/10.3390/mi15050617 - 30 Apr 2024
Viewed by 156
Abstract
This article proposes a novel design for an in-memory computing SRAM, the DAM SRAM CORE, which integrates storage and computational functionality within a unified 11T SRAM cell and enables the performance of large-scale parallel Multiply–Accumulate (MAC) operations within the SRAM array. This design [...] Read more.
This article proposes a novel design for an in-memory computing SRAM, the DAM SRAM CORE, which integrates storage and computational functionality within a unified 11T SRAM cell and enables the performance of large-scale parallel Multiply–Accumulate (MAC) operations within the SRAM array. This design not only improves the area efficiency of the individual cells but also realizes a compact layout. A key highlight of this design is its employment of a dynamic aXNOR-based computation mode, which significantly reduces the consumption of both dynamic and static power during the computational process within the array. Additionally, the design innovatively incorporates a self-stabilizing voltage gradient quantization circuit, which enhances the computational accuracy of the overall system. The 64 × 64 bit DAM SRAM CORE in-memory computing core was fabricated using the 55 nm CMOS logic process and validated via simulations. The experimental results show that this core can deliver 5-bit output results with 1-bit input feature data and 1-bit weight data, while maintaining a static power consumption of 0.48 mW/mm2 and a computational power consumption of 11.367 mW/mm2. This showcases its excellent low-power characteristics. Furthermore, the core achieves a data throughput of 109.75 GOPS and exhibits an impressive energy efficiency of 21.95 TOPS/W, which robustly validate the effectiveness and advanced nature of the proposed in-memory computing core design. Full article
(This article belongs to the Special Issue Emerging Memory Materials and Devices)
19 pages, 3328 KiB  
Article
Dimension Prediction and Microstructure Study of Wire Arc Additive Manufactured 316L Stainless Steel Based on Artificial Neural Network and Finite Element Simulation
by Yanyan Di, Zhizhen Zheng, Shengyong Pang, Jianjun Li and Yang Zhong
Micromachines 2024, 15(5), 615; https://doi.org/10.3390/mi15050615 - 30 Apr 2024
Viewed by 174
Abstract
The dimensional accuracy and microstructure affect the service performance of parts fabricated by wire arc additive manufacturing (WAAM). Regulating the geometry and microstructure of such parts presents a challenge. The coupling method of an artificial neural network and finite element (FE) is proposed [...] Read more.
The dimensional accuracy and microstructure affect the service performance of parts fabricated by wire arc additive manufacturing (WAAM). Regulating the geometry and microstructure of such parts presents a challenge. The coupling method of an artificial neural network and finite element (FE) is proposed in this research for this purpose. Back-propagating neural networks (BPNN) based on optimization algorithms were established to predict the bead width (BW) and height (BH) of the deposited layers. Then, the bead geometry was modeled based on the predicted dimension, and 3D FE heat transfer simulation was performed to investigate the evolution of temperature and microstructure. The results showed that the errors in BW and BH were less than 6%, and the beetle antenna search BPNN model had the highest prediction accuracy compared to the other models. The simulated melt pool error was less than 5% with the experimental results. The decrease in the ratio of the temperature gradient and solidification rate induced the transition of solidified grains from cellular crystals to columnar dendrites and then to equiaxed dendrites. Accelerating the cooling rate increased the primary dendrite arm spacing and δ-ferrite content. These results indicate that the coupling model provides a pathway for regulating the dimensions and microstructures of manufactured parts. Full article
13 pages, 1250 KiB  
Article
Investigation on the Deformation and Surface Quality of a Bearing Outer Ring during Grinding Processing
by Jiang Guo, Pengyu Long, Yong Zhao, Haojie Xu, Zhaoyuan Yang, Jianjun Wang, Tingting Li and Jiwu Tang
Micromachines 2024, 15(5), 614; https://doi.org/10.3390/mi15050614 - 30 Apr 2024
Viewed by 190
Abstract
Thin-walled bearings are widely used owing to the advantages of their light structure, high hardness, and strong load-carrying capacity. However, thin-walled bearings are often prone to deformation during the machining process, which can seriously affect the performance of the bearings. In addition, the [...] Read more.
Thin-walled bearings are widely used owing to the advantages of their light structure, high hardness, and strong load-carrying capacity. However, thin-walled bearings are often prone to deformation during the machining process, which can seriously affect the performance of the bearings. In addition, the machining deformation and quality of bearings are difficult to balance. To address the above issues, this paper investigates the effects of the machining parameters on the machining deformation, surface quality, and machining efficiency of a thin-walled bearing during the roughing stage. The dynamic balance between deformation inhibition and high quality in rough grinding was studied, and the optimal parameters for thin-walled bearing outer ring grinding were obtained. The deformation mechanism of thin-walled bearings caused by grinding was revealed through simulation and experimental analysis. The results show that the machining deformation and quality reach a balance when the workpiece speed is 55 r/min, the grinding wheel rotational speed is 2000 r/min, and the feed rate is 0.1 mm/min. Deformation increases with the increase in workpiece speed and grinding wheel speed. At the same time, the surface roughness increases with the increase in the workpiece speed, but the increase in the wheel speed will improve the surface roughness. As the workpiece speed increases, the surface topography shows a more pronounced stockpile of material, which is ameliorated by an increase in grinding wheel speed. As the rotational speed of the workpiece increases, the number of abrasive grains involved in the process per unit of time decreases, and the surface removal of the workpiece is less effective, while the increase in the rotational speed of the grinding wheel has the opposite effect. The grinding deformation of thin-walled bearings is mainly induced by machining heat and stress. As the rotational speed increases, the heat flux in the grinding zone increases. More heat flux flows into the surface of the workpiece, causing an increase in thermal stresses on the inner surface of the bearing collar, leading to greater deformation. The temperature in the grinding area can be reduced during machining, realizing a reduction in deformation. The research content contributes to the balance between high quality and low distortion in machining processes. Full article
(This article belongs to the Section D:Materials and Processing)
15 pages, 1156 KiB  
Article
Enhancement of Convection and Molecular Transport into Film Stacked Structures by Introduction of Notch Shape for Micro-Immunoassay
by Daiki Arai, Satoshi Ogata, Tetsuhide Shimizu and Ming Yang
Micromachines 2024, 15(5), 613; https://doi.org/10.3390/mi15050613 - 30 Apr 2024
Viewed by 112
Abstract
A 3D-stack microfluidic device that can be used in combination with 96-well plates for micro-immunoassay was developed by the authors. ELISA for detecting IgA by the 3D-stack can be performed in one-ninth of the time of the conventional method by using only 96-well [...] Read more.
A 3D-stack microfluidic device that can be used in combination with 96-well plates for micro-immunoassay was developed by the authors. ELISA for detecting IgA by the 3D-stack can be performed in one-ninth of the time of the conventional method by using only 96-well plates. In this study, a notched-shape film was designed and utilized for the 3D-stack to promote circulation by enhancing and utilizing the axial flow and circumferential flow in order to further reduce the reaction time. A finite element analysis was performed to evaluate the axial flow and circumferential flow while using the 3D-stack in a well and design the optimal shape. The 3D-stack with the notched-shape film was fabricated and utilized for the binding rate test of the antibody and antigen and ELISA. As a result, by promoting circulation using 3D-stack with notched-shape film, the reaction time for each process of ELISA was reduced to 1 min, which is 1/60 for 96 wells at low concentrations. Full article
(This article belongs to the Special Issue Recent Progress of Lab-on-a-Chip Assays)
12 pages, 6700 KiB  
Article
Impact of Hydrogen Voiding in Chip-to-Chip Electroless All-Copper Interconnections
by Nana Ren, Yuyi Zhang, Wenlong Shu, Chenxiao Lu, Wenjing Zhang, Zhuo Chen and Fuliang Wang
Micromachines 2024, 15(5), 612; https://doi.org/10.3390/mi15050612 - 30 Apr 2024
Viewed by 193
Abstract
Three-dimensional (3D) integration has become a leading approach in chip packaging. The interconnection density and reliability of micro-bumps in chip stacking are often threatened by high bonding temperatures. The method of building chip-to-chip interconnections by electroless deposition of metal has its distinct merit, [...] Read more.
Three-dimensional (3D) integration has become a leading approach in chip packaging. The interconnection density and reliability of micro-bumps in chip stacking are often threatened by high bonding temperatures. The method of building chip-to-chip interconnections by electroless deposition of metal has its distinct merit, while the interfacial defect issue, especially that related to voiding during the merging of opposite sides, remains largely unsolved. In this study, to trace the influencing factors in the voiding, the growth characteristics of the electroless all-copper interconnections were examined by carrying out deposition experiments in a microfluidic channel device. The results show that when the gap between the opposite copper bumps to be electrolessly merged is as low as 10 μm, significant voids appear at the inflow side and the top of the copper bumps because the hydrogen cannot be expelled in time. A finite-element flow model of the plating solution between the chips was established, which showed that the flow rate of the plating solution around the copper bumps was much higher than in the merging gap, causing an uneven supply of reactants. Based on these findings, we proposed two potential solutions, one is to improve the flow mode of the plating solution, and the other is to add the reaction inhibitor, 2,2′-bipyridine. Finally, the combination of these two approaches successfully achieved an improved merging quality of the copper joints. Full article
(This article belongs to the Section E:Engineering and Technology)
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9 pages, 2828 KiB  
Article
Effect of High-Temperature Storage on Electrical Characteristics of Hydrogen-Treated AlGaN/GaN High-Electron-Mobility Transistors
by Bin Zhou, Chang Liu, Chenrun Guo, Xianghong Hu, Xiaodong Jian, Hongyue Wang and Xiaofeng Yang
Micromachines 2024, 15(5), 611; https://doi.org/10.3390/mi15050611 - 30 Apr 2024
Viewed by 148
Abstract
In this paper, high-temperature storage of hydrogen-treated AlGaN/GaN HEMTs is conducted for the first time to study the effect of high temperature on the electrical characteristics of the devices after hydrogen treatment, and it is found that high-temperature storage can effectively reduce the [...] Read more.
In this paper, high-temperature storage of hydrogen-treated AlGaN/GaN HEMTs is conducted for the first time to study the effect of high temperature on the electrical characteristics of the devices after hydrogen treatment, and it is found that high-temperature storage can effectively reduce the impact of hydrogen on the devices. After hydrogen treatment, the output current and the maximum transconductance of the device increase, and the threshold voltage drifts negatively. However, after high-temperature treatment at 200 °C for 24 h, the output current, threshold voltage, and the maximum transconductance of the device all approach their initial values before hydrogen treatment. By using low-frequency noise analysis technology, the trap density of the hydrogen-treated AlGaN/GaN HEMT is determined to be 8.9 × 1023 cm−3·eV−1, while it changes to 4.46 × 1022 cm−3·eV−1 after high-temperature storage. We believe that the change in the electrical characteristics of the device in hydrogen is due to the passivation of hydrogen on the inherent trap of the device, and the variation in the electrical properties of the device in the process of high-temperature storage involves the influence of two effects, namely the dehydrogenation effect and the improvement of the metal–semiconductor interface caused by high temperatures. Full article
(This article belongs to the Section D1: Semiconductor Devices)
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11 pages, 2654 KiB  
Article
Racetrack Ring Resonator-Based on Hybrid Plasmonic Waveguide for Refractive Index Sensing
by Muhammad A. Butt
Micromachines 2024, 15(5), 610; https://doi.org/10.3390/mi15050610 - 30 Apr 2024
Viewed by 261
Abstract
In this study, a comprehensive numerical analysis is conducted on a hybrid plasmonic waveguide (HPWG)-based racetrack ring resonator (RTRR) structure, tailored specifically for refractive index sensing applications. The sensor design optimization yields remarkable results, achieving a sensitivity of 275.7 nm/RIU. Subsequently, the boundaries [...] Read more.
In this study, a comprehensive numerical analysis is conducted on a hybrid plasmonic waveguide (HPWG)-based racetrack ring resonator (RTRR) structure, tailored specifically for refractive index sensing applications. The sensor design optimization yields remarkable results, achieving a sensitivity of 275.7 nm/RIU. Subsequently, the boundaries of sensor performance are pushed even further by integrating a subwavelength grating (SWG) structure into the racetrack configuration, thereby augmenting the light–matter interaction. Of particular note is the pivotal role played by the length of the SWG segment in enhancing device sensitivity. It is observed that a significant sensitivity enhancement can be obtained, with values escalating from 377.1 nm/RIU to 477.7 nm/RIU as the SWG segment length increases from 5 µm to 10 µm, respectively. This investigation underscores the immense potential of HPWG in tandem with SWG for notably enhancing the sensitivity of photonic sensors. These findings not only advance the understanding of these structures but also pave the way for the development of highly efficient sensing devices with unprecedented performance capabilities. Full article
(This article belongs to the Special Issue Advances in Sensors and Electronic Instrumentation 2024)
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19 pages, 5535 KiB  
Article
MEMS Gyroscope Temperature Compensation Based on Improved Complete Ensemble Empirical Mode Decomposition and Optimized Extreme Learning Machine
by Zhihao Zhang, Jintao Zhang, Xiaohan Zhu, Yanchao Ren, Jingfeng Yu and Huiliang Cao
Micromachines 2024, 15(5), 609; https://doi.org/10.3390/mi15050609 - 30 Apr 2024
Viewed by 199
Abstract
Herein, we investigate the temperature compensation for a dual-mass MEMS gyroscope. After introducing and simulating the dual-mass MEMS gyroscope’s working modes, we propose a hybrid algorithm for temperature compensation relying on improved complete ensemble empirical mode decomposition with adaptive noise (ICEEMDAN), sample entropy, [...] Read more.
Herein, we investigate the temperature compensation for a dual-mass MEMS gyroscope. After introducing and simulating the dual-mass MEMS gyroscope’s working modes, we propose a hybrid algorithm for temperature compensation relying on improved complete ensemble empirical mode decomposition with adaptive noise (ICEEMDAN), sample entropy, time–frequency peak filtering, non-dominated sorting genetic algorithm-II (NSGA II) and extreme learning machine. Firstly, we use ICEEMDAN to decompose the gyroscope’s output signal, and then we use sample entropy to classify the decomposed signals. For noise segments and mixed segments with different levels of noise, we use time–frequency peak filtering with different window lengths to achieve a trade-off between noise removal and signal retention. For the feature segment with temperature drift, we build a compensation model using extreme learning machine. To improve the compensation accuracy, NSGA II is used to optimize extreme learning machine, with the prediction error and the 2-norm of the output-layer connection weight as the optimization objectives. Enormous simulation experiments prove the excellent performance of our proposed scheme, which can achieve trade-offs in signal decomposition, classification, denoising and compensation. The improvement in the compensated gyroscope’s output signal is analyzed based on Allen variance; its angle random walk is decreased from 0.531076°/h/√Hz to 6.65894 × 10−3°/h/√Hz and its bias stability is decreased from 32.7364°/h to 0.259247°/h. Full article
(This article belongs to the Section E:Engineering and Technology)
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20 pages, 9156 KiB  
Article
Design and Testing of a Compliant ZTTΘ Positional Adjustment System with Hybrid Amplification
by Zhishen Liao, Zhihang Lin, Hui Tang, Bo Liu and Yingjie Jia
Micromachines 2024, 15(5), 608; https://doi.org/10.3390/mi15050608 - 30 Apr 2024
Viewed by 219
Abstract
This article presents the design, analysis, and prototype testing of a four-degrees-of-freedom (4-DoFs) spatial pose adjustment system (SPAS) that achieves high-precision positioning with 4-DoFs (Z/Tip/Tilt/Θ). The system employs a piezoelectric-driven amplification mechanism that combines a bridge lever hybrid amplification mechanism, a [...] Read more.
This article presents the design, analysis, and prototype testing of a four-degrees-of-freedom (4-DoFs) spatial pose adjustment system (SPAS) that achieves high-precision positioning with 4-DoFs (Z/Tip/Tilt/Θ). The system employs a piezoelectric-driven amplification mechanism that combines a bridge lever hybrid amplification mechanism, a double four-bar guide mechanism, and a multi-level lever symmetric rotation mechanism. By integrating these mechanisms, the system achieves low coupling, high stiffness, and wide stroke range. Analytical modeling and finite element analysis are employed to optimize geometric parameters. A prototype is fabricated, and its performance is verified through testing. The results indicate that the Z-direction feed microstroke is 327.37 μm, the yaw motion angle around the X and Y axes is 3.462 mrad, and the rotation motion angle around the Z axis is 12.684 mrad. The x-axis and y-axis motion magnification ratio can reach 7.43. Closed-loop decoupling control experiments for multiple-input-multiple-outputs (MIMO) systems using inverse kinematics and proportional-integral-derivative feedback controllers were conducted. The results show that the Z-direction positioning accuracy is ±100 nm, the X and Y axis yaw motion accuracy is ±2 μrad, and the Z-axis rotation accuracy is ±25 μrad. Due to the ZTTΘ mechanism, the design proved to be feasible and advantageous, demonstrating its potential for precision machining and micro-nano manipulation. Full article
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4 pages, 154 KiB  
Editorial
Editorial for Special Issue on Biosensors for Biomedical and Environmental Applications
by Antonella Battisti
Micromachines 2024, 15(5), 607; https://doi.org/10.3390/mi15050607 - 30 Apr 2024
Viewed by 174
Abstract
A sensor is typically defined as a device able to transform a physical quantity of interest into a different kind of signal that can be easily measured and recorded [...] Full article
(This article belongs to the Special Issue Biosensors for Biomedical and Environmental Applications)
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8 pages, 3112 KiB  
Communication
Fast and Massive Pixel-Level Morphology Detection by Imaging Processing for Inkjet Printing
by Haoyang Zhang, Da Xu, Shanrong Ke, Meicong Huang, Yaling Chai, Yi Lin, Ziquan Guo and Zhong Chen
Micromachines 2024, 15(5), 606; https://doi.org/10.3390/mi15050606 - 30 Apr 2024
Viewed by 220
Abstract
With the rapid development of the emerging intelligent, flexible, transparent, and wearable electronic devices, such as quantum-dot-based micro light-emitting diodes (micro-LEDs), thin-film transistors (TFTs), and flexible sensors, numerous pixel-level printing technologies have emerged. Among them, inkjet printing has proven to be a useful [...] Read more.
With the rapid development of the emerging intelligent, flexible, transparent, and wearable electronic devices, such as quantum-dot-based micro light-emitting diodes (micro-LEDs), thin-film transistors (TFTs), and flexible sensors, numerous pixel-level printing technologies have emerged. Among them, inkjet printing has proven to be a useful and effective tool for consistently printing micron-level ink droplets, for instance, smaller than 50 µm, onto wearable electronic devices. However, quickly and accurately determining the printing quality, which is significant for the electronic device performance, is challenging due to the large quantity and micron size of ink droplets. Therefore, leveraging existing image processing algorithms, we have developed an effective method and software for quickly detecting the morphology of printed inks served in inkjet printing. This method is based on the edge detection technology. We believe this method can greatly meet the increasing demands for quick evaluation of print quality in inkjet printing. Full article
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11 pages, 3764 KiB  
Article
Enhancing the Uniformity of a Memristor Using a Bilayer Dielectric Structure
by Yulin Liu, Qilai Chen, Yanbo Guo, Bingjie Guo, Gang Liu, Yanchao Liu, Lei He, Yutong Li, Jingyan He and Minghua Tang
Micromachines 2024, 15(5), 605; https://doi.org/10.3390/mi15050605 - 30 Apr 2024
Viewed by 255
Abstract
Resistive random access memory (RRAM) holds great promise for in-memory computing, which is considered the most promising strategy for solving the von Neumann bottleneck. However, there are still significant problems in its application due to the non-uniform performance of RRAM devices. In this [...] Read more.
Resistive random access memory (RRAM) holds great promise for in-memory computing, which is considered the most promising strategy for solving the von Neumann bottleneck. However, there are still significant problems in its application due to the non-uniform performance of RRAM devices. In this work, a bilayer dielectric layer memristor was designed based on the difference in the Gibbs free energy of the oxide. We fabricated Au/Ta2O5/HfO2/Ta/Pt (S3) devices with excellent uniformity. Compared with Au/HfO2/Pt (S1) and Au/Ta2O5/Pt (S2) devices, the S3 device has a low reset voltage fluctuation of 2.44%, and the resistive coefficients of variation are 13.12% and 3.84% in HRS and LRS, respectively, over 200 cycles. Otherwise, the bilayer device has better linearity and more conductance states in multi-state regulation. At the same time, we analyze the physical mechanism of the bilayer device and provide a physical model of ion migration. This work provides a new idea for designing and fabricating resistive devices with stable performance. Full article
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4 pages, 655 KiB  
Editorial
Editorial for the Special Issue on Biomaterials, Biodevices and Tissue Engineering
by Luis Jesús Villarreal-Gómez, José Manuel Cornejo-Bravo and Faruk Fonthal
Micromachines 2024, 15(5), 604; https://doi.org/10.3390/mi15050604 - 30 Apr 2024
Viewed by 252
Abstract
Biomaterials, biodevices, and tissue engineering represent the cutting edge of medical science, promising revolutionary solutions to some of humanity’s most pressing health challenges (Figure 1) [...] Full article
(This article belongs to the Special Issue Biomaterials, Biodevices and Tissue Engineering)
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14 pages, 4983 KiB  
Article
Design, Modeling, and Experimental Validation of an Active Microcatheter Driven by Shape Memory Effects
by Chengyang Li, Xu Zhang, Zhongjing Ren, Jingkai Wang, Shouyu Sun, Jian Fu, Yang Xu and Wu Duan
Micromachines 2024, 15(5), 603; https://doi.org/10.3390/mi15050603 - 30 Apr 2024
Viewed by 234
Abstract
Microcatheters capable of active guidance have been proven to be effective and efficient solutions to interventional surgeries for cardiovascular and cerebrovascular diseases. Herein, a novel microcatheter made of two biocompatible materials, shape memory alloy (SMA) and polyethylene (PE), is proposed. It consists of [...] Read more.
Microcatheters capable of active guidance have been proven to be effective and efficient solutions to interventional surgeries for cardiovascular and cerebrovascular diseases. Herein, a novel microcatheter made of two biocompatible materials, shape memory alloy (SMA) and polyethylene (PE), is proposed. It consists of a reconfigurable distal actuator and a separate polyethylene catheter. The distal actuator is created via embedding U-shape SMA wires into the PE base, and its reconfigurability is mainly dominated by the shape memory effect (SME) of SMA wires, as well as the effect of thermal mismatch between the SMA and PE base. A mathematical model was established to predict the distal actuator’s deformation, and the analytical solutions show great agreement with the finite element results. Structural optimization of such microcatheters was carried out using the verified analytical model, followed by fabrication of some typical prototypes. Experimental testing of their mechanical behaviors demonstrates the feasibility of the structural designs, and the reliability and accuracy of the mathematical model. The active microcatheter, together with the prediction model, will lay a solid foundation for rapid development and optimization of active navigation strategies for vascular interventions. Full article
(This article belongs to the Special Issue N/MEMS Intelligent Structures: Design, Manufacturing, and Control)
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15 pages, 1722 KiB  
Article
Designing a Simple Electrochemical Genosensor for the Detection of Urinary PCA3, a Prostate Cancer Biomarker
by Meriem Mokni, Amal Tlili, Yassine Khalij, Ghada Attia, Chouki Zerrouki, Wissem Hmida, Ali Othmane, Ali Bouslama, Asma Omezzine and Najla Fourati
Micromachines 2024, 15(5), 602; https://doi.org/10.3390/mi15050602 - 29 Apr 2024
Viewed by 224
Abstract
This study investigates the feasibility of a simple electrochemical detection of Prostate Cancer Antigen 3 (PCA3) fragments extracted from patients’ urine, using a thiolated single-strand DNA probe immobilized on a gold surface without using a redox probe. To enhance the PCA3 recognition process, [...] Read more.
This study investigates the feasibility of a simple electrochemical detection of Prostate Cancer Antigen 3 (PCA3) fragments extracted from patients’ urine, using a thiolated single-strand DNA probe immobilized on a gold surface without using a redox probe. To enhance the PCA3 recognition process, we conducted a comparative analysis of the hybridization location using two thiolated DNA probes: Probe 1 targets the first 40 bases, while Probe 2 targets the fragment from bases 47 to 86. Hybridization with PCA3 followed, using square wave voltammetry. The limit of detection of the designed genosenors were of the order of (2.2 ng/mL), and (1.6 ng/mL) for Probes 1 and 2, respectively, and the subsequent sensitivities were of the order of (0.09 ± 0.01) µA−1 · µg−1 · mL and (0.10 ± 0.01) µA−1 · µg−1 · mL. Specificity tests were then conducted with the sensor functionalized with Probe 2, as it presents better analytical performances. The electrochemical results indicate that the designed sensor can clearly discriminate a complementary target from a non-complementary one. A further modeling of the calibration curves with the Power Law/Hill model indicates that the dissociation constant increases by one order of magnitude, confirming the ability of the designed sensor to perfectly discriminate complementary targets from non-complementary ones. Full article
10 pages, 1771 KiB  
Article
Design of A Compact 2–6 GHz High-Efficiency and High-Gain GaN Power Amplifier
by Yongchun Zhou, Shuai Wang, Junyan Dai, Jiang Luo and Qiang Cheng
Micromachines 2024, 15(5), 601; https://doi.org/10.3390/mi15050601 - 29 Apr 2024
Viewed by 191
Abstract
In this paper, a novel wideband power amplifier (PA) operating in the 2–6 GHz frequency range is presented. The proposed PA design utilizes a combination technique consisting of a distributed equalization technique, multiplexing the power supply network and matching network technique, an LR [...] Read more.
In this paper, a novel wideband power amplifier (PA) operating in the 2–6 GHz frequency range is presented. The proposed PA design utilizes a combination technique consisting of a distributed equalization technique, multiplexing the power supply network and matching network technique, an LR dissipative structure, and an RC stability network technique to achieve significant bandwidth while maintaining superior gain flatness, high efficiency, high gain, and compact size. For verification, a three-stage PA using the combination technique is designed and implemented in a 0.25 μm GaN high-electron-mobility transistor (HEMT) process. The fabricated prototype demonstrates a saturated output power of 4 W, a power gain of 21 dB, a gain flatness of ±0.6 dB, a power-added efficiency of 39–46%, and a fractional bandwidth of 100% under the operating conditions of drain voltage 28 V (continuous wave) and gate voltage −2.6 V. Moreover, the chip occupies a compact size of only 2.51 mm × 1.97 mm. Full article
14 pages, 762 KiB  
Article
Research on the Influence of Carbon Sources and Buffer Layers on the Homogeneous Epitaxial Growth of 4H-SiC
by Weilong Yuan, Yicheng Pei, Yunkai Li, Ning Guo, Xiuhai Zhang and Xingfang Liu
Micromachines 2024, 15(5), 600; https://doi.org/10.3390/mi15050600 - 29 Apr 2024
Viewed by 178
Abstract
In this study, a 4H-SiC homoepitaxial layer was grown on a 150 mm 4° off-axis substrate using a horizontal hot wall chemical vapor deposition reactor. Comparing C3H8 and C2H4 as C sources, the sample grown with C [...] Read more.
In this study, a 4H-SiC homoepitaxial layer was grown on a 150 mm 4° off-axis substrate using a horizontal hot wall chemical vapor deposition reactor. Comparing C3H8 and C2H4 as C sources, the sample grown with C2H4 exhibited a slower growth rate and lower doping concentration, but superior uniformity and surface roughness compared to the C3H8-grown sample. Hence, C2H4 is deemed more suitable for commercial epitaxial wafer growth. Increasing growth pressure led to decreased growth rate, worsened thickness uniformity, reduced doping concentration, deteriorated uniformity, and initially improved and then worsened surface roughness. Optimal growth quality was observed at a lower growth pressure of 40 Torr. Furthermore, the impact of buffer layer growth on epitaxial quality varied significantly based on different C/Si ratios, emphasizing the importance of selecting the appropriate conditions for subsequent device manufacturing. Full article
(This article belongs to the Section D1: Semiconductor Devices)
12 pages, 2584 KiB  
Article
Fabrication of Perfluoropolyether Microfluidic Devices Using Laser Engraving for Uniform Droplet Production
by Eun Seo Kim, Mincheol Cho, Inseong Choi and Sung-Wook Choi
Micromachines 2024, 15(5), 599; https://doi.org/10.3390/mi15050599 - 29 Apr 2024
Viewed by 243
Abstract
A perfluoropolyether (PFPE)-based microfluidic device with cross-junction microchannels was fabricated with the purpose of producing uniform droplets. The microchannels were developed using CO2 laser engraving. PFPE was chosen as the main material because of its excellent solvent resistance. Polyethylene glycol diacrylate (PEGDA) [...] Read more.
A perfluoropolyether (PFPE)-based microfluidic device with cross-junction microchannels was fabricated with the purpose of producing uniform droplets. The microchannels were developed using CO2 laser engraving. PFPE was chosen as the main material because of its excellent solvent resistance. Polyethylene glycol diacrylate (PEGDA) was mixed with PFPE to improve the hydrophilic properties of the inner surface of the microchannels. The microchannels of the polydimethylsiloxane microfluidic device had a blackened and rough surface after laser engraving. By contrast, the inner surface of the microchannels of the PFPE-PEGDA microfluidic device exhibited a smooth surface. The lower power and faster speed of the laser engraving resulted in the development of microchannels with smaller dimensions, less than 30 μm in depth. The PFPE and PFPE-PEGDA microfluidic devices were used to produce uniform water and oil droplets, respectively. We believe that such a PFPE-based microfluidic device with CO2-laser-engraved microchannels can be used as a microfluidic platform for applications in various fields, such as biological and chemical analysis, extraction, and synthesis. Full article
(This article belongs to the Collection Lab-on-a-Chip)
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12 pages, 5794 KiB  
Article
Skin Electrodes Based on TPU Fiber Scaffolds with Conductive Nanocomposites with Stretchability, Breathability, and Washability
by Zijia Zhao, Chaopeng Yang and Dongchan Li
Micromachines 2024, 15(5), 598; https://doi.org/10.3390/mi15050598 - 29 Apr 2024
Viewed by 206
Abstract
In the context of an aging population and escalating work pressures, cardiovascular diseases pose increasing health risks. Electrocardiogram (ECG) monitoring presents a preventive tool, but conventional devices often compromise comfort. This study proposes an approach using Ag NW/TPU composites for flexible and breathable [...] Read more.
In the context of an aging population and escalating work pressures, cardiovascular diseases pose increasing health risks. Electrocardiogram (ECG) monitoring presents a preventive tool, but conventional devices often compromise comfort. This study proposes an approach using Ag NW/TPU composites for flexible and breathable epidermal electronics. In this new structure, TPU fibers are used to support Ag NWs/TPU nanocomposites. The TPU fiber-reinforced Ag NW/TPU (TFRAT) nanocomposites exhibit excellent conductivity, stretchability, and electromechanical durability. The composite ensures high steam permeability, maintaining stable electrical performance after washing cycles. Employing this technology, a flexible ECG detection system is developed, augmented with a convolutional neural network (CNN) for automated signal analysis. The experimental results demonstrate the system’s reliability in capturing physiological signals. Additionally, a CNN model trained on ECG data achieves over 99% accuracy in diagnosing arrhythmias. This study presents TFRAT as a promising solution for wearable electronics, offering both comfort and functionality in long-term epidermal applications, with implications for healthcare and beyond. Full article
(This article belongs to the Special Issue Flexible and Wearable Sensors, 3rd Edition)
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12 pages, 5496 KiB  
Article
Improved Test Fixture for Collecting Microcontact Performance and Reliability Data
by Turja Nandy, Ronald A. Coutu, Jr. and Rafee Mahbub
Micromachines 2024, 15(5), 597; https://doi.org/10.3390/mi15050597 - 29 Apr 2024
Viewed by 245
Abstract
Microelectromechanical systems (MEMS) ohmic contact switches are considered to be a promising candidate for wireless communication applications. The longevity of MEMS switches is directly related to the reliability and performance of microcontacts. In this work, an improved microcontact test fixture with high actuation [...] Read more.
Microelectromechanical systems (MEMS) ohmic contact switches are considered to be a promising candidate for wireless communication applications. The longevity of MEMS switches is directly related to the reliability and performance of microcontacts. In this work, an improved microcontact test fixture with high actuation rates (KHz) and highly precise position control (nm) and force (nN) control was developed. Here, we collected microcontact performance data from initial contact tests (ICT) and microcontact reliability data from cold switched tests (CST). To perform these tests with our test fixture, we fabricated MEMS microcontact test structures with relatively high Young’s modulus electroplated Nickel (Ni)-based, fixed–fixed beam structure with Au/RuO2 bimetallic microcontacts. These structures were characterized for forces ranging from 200–1000 µN in ICT tests. In a CST test, the tested microcontact survived more than 200 million cycles at a 1 KHz cycle rate, with a stable contact resistance value ranging between 3.8–5.2 Ω. These experiments validate the potentiality of our microcontact test fixture, and will facilitate further investigation on advanced microcontacts to enhance the MEMS switch’s reliability. Full article
(This article belongs to the Special Issue Feature Papers from Micromachines Reviewers 2023)
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12 pages, 5116 KiB  
Article
Design and Growth of Low Resistivity P-Type AlGaN Superlattice Structure
by Yang Liu, Xiaowei Zhou, Peixian Li, Bo Yang and Zhuang Zhao
Micromachines 2024, 15(5), 596; https://doi.org/10.3390/mi15050596 - 29 Apr 2024
Viewed by 213
Abstract
This work investigated the impact of periodic thickness and doping region on the doping efficiency of the P-type AlGaN superlattice. In this paper, the band structure of the simulated superlattice was analyzed. The superlattice structure of Al0.1Ga0.3N/Al0.4Ga [...] Read more.
This work investigated the impact of periodic thickness and doping region on the doping efficiency of the P-type AlGaN superlattice. In this paper, the band structure of the simulated superlattice was analyzed. The superlattice structure of Al0.1Ga0.3N/Al0.4Ga0.6N, and the AlGaN buffer on the sapphire substrate, achieved a resistivity of ~3.3 Ω·cm. The results indicate that barrier doping and low periodic thickness offer significant advantages in introducing a reduction of the resistivity of P-type AlGaN superlattice structures. Full article
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14 pages, 9925 KiB  
Article
Modulation Steering Motion by Quantitative Electrical Stimulation in Pigeon Robots
by Mingxuan Bi, Huimin Zhang, Yaohong Ma, Hao Wang, Wenbo Wang, Yuan Shi, Wenlong Sheng, Qiushun Li, Guangheng Gao and Lei Cai
Micromachines 2024, 15(5), 595; https://doi.org/10.3390/mi15050595 - 29 Apr 2024
Viewed by 278
Abstract
The pigeon robot has attracted significant attention in the field of animal robotics thanks to its outstanding mobility and adaptive capability in complex environments. However, research on pigeon robots is currently facing bottlenecks, and achieving fine control over the motion behavior of pigeon [...] Read more.
The pigeon robot has attracted significant attention in the field of animal robotics thanks to its outstanding mobility and adaptive capability in complex environments. However, research on pigeon robots is currently facing bottlenecks, and achieving fine control over the motion behavior of pigeon robots through brain–machine interfaces remains challenging. Here, we systematically quantify the relationship between electrical stimulation and stimulus-induced motion behaviors, and provide an analytical method to demonstrate the effectiveness of pigeon robots based on electrical stimulation. In this study, we investigated the influence of gradient voltage intensity (1.2–3.0 V) on the indoor steering motion control of pigeon robots. Additionally, we discussed the response time of electrical stimulation and the effective period of the brain–machine interface. The results indicate that pigeon robots typically exhibit noticeable behavioral responses at a 2.0 V voltage stimulus. Increasing the stimulation intensity significantly controls the steering angle and turning radius (p < 0.05), enabling precise control of pigeon robot steering motion through stimulation intensity regulation. When the threshold voltage is reached, the average response time of a pigeon robot to the electrical stimulation is 220 ms. This study quantifies the role of each stimulation parameter in controlling pigeon robot steering behavior, providing valuable reference information for the precise steering control of pigeon robots. Based on these findings, we offer a solution for achieving precise control of pigeon robot steering motion and contribute to solving the problem of encoding complex trajectory motion in pigeon robots. Full article
(This article belongs to the Special Issue Intelligent Bioelectronics and Neural Interfaces)
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12 pages, 2421 KiB  
Article
Flexible Peripheral Nerve Interfacing Electrode for Joint Position Control in Closed-Loop Neuromuscular Stimulation
by Sia Kim and Kang-Il Song
Micromachines 2024, 15(5), 594; https://doi.org/10.3390/mi15050594 - 29 Apr 2024
Viewed by 225
Abstract
Addressing peripheral nerve disorders with electronic medicine poses significant challenges, especially in replicating the dynamic mechanical properties of nerves and understanding their functionality. In the field of electronic medicine, it is crucial to design a system that thoroughly understands the functions of the [...] Read more.
Addressing peripheral nerve disorders with electronic medicine poses significant challenges, especially in replicating the dynamic mechanical properties of nerves and understanding their functionality. In the field of electronic medicine, it is crucial to design a system that thoroughly understands the functions of the nervous system and ensures a stable interface with nervous tissue, facilitating autonomous neural adaptation. Herein, we present a novel neural interface platform that modulates the peripheral nervous system using flexible nerve electrodes and advanced neuromodulation techniques. Specifically, we have developed a surface-based inverse recruitment model for effective joint position control via direct electrical nerve stimulation. Utilizing barycentric coordinates, this model constructs a three-dimensional framework that accurately interpolates inverse isometric recruitment values across various joint positions, thereby enhancing control stability during stimulation. Experimental results from rabbit ankle joint control trials demonstrate our model’s effectiveness. In combination with a proportional–integral–derivative (PID) controller, it shows superior performance by achieving reduced settling time (less than 1.63 s), faster rising time (less than 0.39 s), and smaller steady-state error (less than 3 degrees) compared to the legacy model. Moreover, the model’s compatibility with recent advances in flexible interfacing technologies and its integration into a closed-loop controlled functional neuromuscular stimulation (FNS) system highlight its potential for precise neuroprosthetic applications in joint position control. This approach marks a significant advancement in the management of neurological disorders with advanced neuroprosthetic solutions. Full article
(This article belongs to the Special Issue Intelligent Bioelectronics and Neural Interfaces)
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17 pages, 4721 KiB  
Article
A Microwave Photonic 2 × 2 IBFD–MIMO Communication System with Narrowband Self-Interference Cancellation
by Ying Ma, Fangjing Shi and Yangyu Fan
Micromachines 2024, 15(5), 593; https://doi.org/10.3390/mi15050593 - 29 Apr 2024
Viewed by 286
Abstract
Combined in-band full duplex-multiple input multiple output (IBFD–MIMO) technology can significantly improve spectrum efficiency and data throughput, and has broad application prospects in communications, radar, the Internet of Things (IoT), and other fields. Targeting the self-interference (SI) issue in microwave photonic-based IBFD–MIMO communication [...] Read more.
Combined in-band full duplex-multiple input multiple output (IBFD–MIMO) technology can significantly improve spectrum efficiency and data throughput, and has broad application prospects in communications, radar, the Internet of Things (IoT), and other fields. Targeting the self-interference (SI) issue in microwave photonic-based IBFD–MIMO communication systems, a microwave photonic self-interference cancellation (SIC) method applied to the narrowband 2 × 2 IBFD–MIMO communication system was proposed, simulated, and analyzed. An interleaver was used to construct a polarization multiplexing dual optical frequency comb with a frequency shifting effect, generating a dual-channel reference interference signal. The programmable spectrum processor was employed for filtering, attenuation, and phase-shifting operations, ensuring amplitude and phase matching to eliminate the two self-interference (SI) signals. The simulation results show that the single-frequency SIC depth exceeds 45.8 dB, and the narrowband SIC depth under 30 MHz bandwidth exceeds 32.7 dB. After SIC, the desired signal, employing a 4QAM modulation format, can be demodulated with an error vector magnitude (EVM) as low as 4.7%. Additionally, further channel expansion and system performance optimization are prospected. Full article
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11 pages, 2822 KiB  
Article
Flexible Thermoelectric Device Based on Protrusion-Structured Liquid Metal Elastomer for Gravity Heat Pipe
by Xiaogang Zhang, Xinghua Zhang, Shaocheng Ge, Bailin Zhang, Dongguang Zhang and Jiayi Yang
Micromachines 2024, 15(5), 592; https://doi.org/10.3390/mi15050592 - 29 Apr 2024
Viewed by 261
Abstract
Monitoring the temperature of the coal gangue mountains is fundamental to preventing their spontaneous combustion. However, the existing temperature monitoring systems fail to achieve stable, pollution-free temperature monitoring without affecting vegetation growth in these mountains. To address this issue, this work proposes a [...] Read more.
Monitoring the temperature of the coal gangue mountains is fundamental to preventing their spontaneous combustion. However, the existing temperature monitoring systems fail to achieve stable, pollution-free temperature monitoring without affecting vegetation growth in these mountains. To address this issue, this work proposes a flexible thermoelectric device (FTD) based on a protrusion-structured liquid metal elastomer (LME). Utilizing a high-thermal-conductivity LME, the FTD adheres closely to the surface of the gravity heat pipe (GHP), ensuring compatibility between FTD and the curved surface of the GHP. Simultaneously, employing a low-thermal-conductivity elastomer helps concentrate heat onto FTD, thereby enhancing thermoelectric power generation efficiency. Additionally, the impact of the shape, size, and height of the protrusion structure at the cold end of the GHP on its efficiency was also investigated. The practical application of FTD on GHP was demonstrated. Full article
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11 pages, 1524 KiB  
Article
Synthesis and Characterization of 2D Ternary Compound TMD Materials Ta3VSe8
by Yuanji Ma, Yuhan Du, Wenbin Wu, Zeping Shi, Xianghao Meng and Xiang Yuan
Micromachines 2024, 15(5), 591; https://doi.org/10.3390/mi15050591 - 28 Apr 2024
Viewed by 286
Abstract
Two-dimensional (2D) transition metal dichalcogenides (TMDs) are garnering considerable scientific interest, prompting discussion regarding their prospective applications in the fields of nanoelectronics and spintronics while also fueling groundbreaking discoveries in phenomena such as the fractional quantum anomalous Hall effect (FQAHE) and exciton dynamics. [...] Read more.
Two-dimensional (2D) transition metal dichalcogenides (TMDs) are garnering considerable scientific interest, prompting discussion regarding their prospective applications in the fields of nanoelectronics and spintronics while also fueling groundbreaking discoveries in phenomena such as the fractional quantum anomalous Hall effect (FQAHE) and exciton dynamics. The abundance of binary compound TMDs, such as MX2 (M = Mo, W; X = S, Se, Te), has unlocked myriad avenues of exploration. However, the exploration of ternary compound TMDs remains relatively limited, with notable examples being Ta2NiS5 and Ta2NiSe5. In this study, we report the synthesis of a new 2D ternary compound TMD materials, Ta3VSe8, employing the chemical vapor transport (CVT) method. The as-grown bulk crystal is shiny and can be easily exfoliated. The crystal quality and structure are verified by X-ray diffraction (XRD), while the surface morphology, stoichiometric ratio, and uniformity are determined by scanning electron microscopy (SEM). Although the phonon property is found stable at different temperatures, magneto-resistivity evolves. These findings provide a possible approach for the realization and exploration of ternary compound TMDs. Full article
(This article belongs to the Special Issue Devices Based on Two-Dimensional Materials: Materials and Fabrication)
15 pages, 3584 KiB  
Article
Mechanical Characterization of the Erythrocyte Membrane Using a Capacitor-Based Technique
by Doriana Dorta, Carlos Plazaola, Jafeth Carrasco, Maria F. Alves-Rosa, Lorena M. Coronado, Ricardo Correa, Maytee Zambrano, Braulio Gutiérrez-Medina, Erick Sarmiento-Gómez, Carmenza Spadafora and Guadalupe Gonzalez
Micromachines 2024, 15(5), 590; https://doi.org/10.3390/mi15050590 - 28 Apr 2024
Viewed by 305
Abstract
Pathological processes often change the mechanical properties of cells. Increased rigidity could be a marker of cellular malfunction. Erythrocytes are a type of cell that deforms to squeeze through tiny capillaries; changes in their rigidity can dramatically affect their functionality. Furthermore, differences in [...] Read more.
Pathological processes often change the mechanical properties of cells. Increased rigidity could be a marker of cellular malfunction. Erythrocytes are a type of cell that deforms to squeeze through tiny capillaries; changes in their rigidity can dramatically affect their functionality. Furthermore, differences in the homeostatic elasticity of the cell can be used as a tool for diagnosis and even for choosing the adequate treatment for some illnesses. More accurate types of equipment needed to study biomechanical phenomena at the single-cell level are very costly and thus out of reach for many laboratories around the world. This study presents a simple and low-cost technique to study the rigidity of red blood cells (RBCs) through the application of electric fields in a hand-made microfluidic chamber that uses a capacitor principle. As RBCs are deformed with the application of voltage, cells are observed under a light microscope. From mechanical force vs. deformation data, the elastic constant of the cells is determined. The results obtained with the capacitor-based method were compared with those obtained using optical tweezers, finding good agreement. In addition, P. falciparum-infected erythrocytes were tested with the electric field applicator. Our technique provides a simple means of testing the mechanical properties of individual cells. Full article
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