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Micromachines, Volume 12, Issue 1

January 2021 - 98 articles

Cover Story: While sorting cells based on their membrane or internal structures has important implications for cell biology, these properties usually do not translate into common properties that have been used for sorting, for example, size, shape, or deformability. We show that by combining electrokinetics with deterministic lateral displacement (DLD) in a microfluidics device, it is possible to sort cells continuously based on differences in their membrane and internal structures. Using heat to deactivate cells, which changes their viability and structure, we demonstrate sorting of a mixture of viable and non-viable cells for two different cell types. We expect our work to form the basis for the development of simple, low-cost, continuous label-free methods that can separate cells and bioparticles based on their intrinsic properties. View this paper.
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Articles (98)

  • Article
  • Open Access
26 Citations
5,042 Views
9 Pages

19 January 2021

Microalgae separation technology is essential for both executing laboratory-based fundamental studies and ensuring the quality of the final algal products. However, the conventional microalgae separation technology of micropipetting requires highly s...

  • Article
  • Open Access
4 Citations
3,996 Views
12 Pages

Parallel Recordings of Transmembrane hERG Channel Currents Based on Solvent-Free Lipid Bilayer Microarray

  • Ryusuke Miyata,
  • Daisuke Tadaki,
  • Daichi Yamaura,
  • Shun Araki,
  • Madoka Sato,
  • Maki Komiya,
  • Teng Ma,
  • Hideaki Yamamoto,
  • Michio Niwano and
  • Ayumi Hirano-Iwata

19 January 2021

The reconstitution of ion-channel proteins in artificially formed bilayer lipid membranes (BLMs) forms a well-defined system for the functional analysis of ion channels and screening of the effects of drugs that act on these proteins. To improve the...

  • Feature Paper
  • Article
  • Open Access
4 Citations
5,022 Views
18 Pages

Micropatterning of Substrates for the Culture of Cell Networks by Stencil-Assisted Additive Nanofabrication

  • Anita Previdi,
  • Claudio Piazzoni,
  • Francesca Borghi,
  • Carsten Schulte,
  • Leandro Lorenzelli,
  • Flavio Giacomozzi,
  • Alessio Bucciarelli,
  • Antonio Malgaroli,
  • Jacopo Lamanna and
  • Andrea Moro
  • + 4 authors

18 January 2021

The fabrication of in vitro neuronal cell networks where cells are chemically or electrically connected to form functional circuits with useful properties is of great interest. Standard cell culture substrates provide ensembles of cells that scarcely...

  • Article
  • Open Access
18 Citations
3,597 Views
6 Pages

Development of a CMOS-Compatible Carbon Nanotube Array Transfer Method

  • Chun Fei Siah,
  • Lucas Yu Xiang Lum,
  • Jianxiong Wang,
  • Simon Chun Kiat Goh,
  • Chong Wei Tan,
  • Liangxing Hu,
  • Philippe Coquet,
  • Hong Li,
  • Chuan Seng Tan and
  • Beng Kang Tay

18 January 2021

Carbon nanotubes (CNTs) have, over the years, been used in research as a promising material in electronics as a thermal interface material and as interconnects amongst other applications. However, there exist several issues preventing the widespread...

  • Review
  • Open Access
62 Citations
8,670 Views
21 Pages

Recent Advances in Three-Dimensional Multicellular Spheroid Culture and Future Development

  • Honglin Shen,
  • Shuxiang Cai,
  • Chuanxiang Wu,
  • Wenguang Yang,
  • Haibo Yu and
  • Lianqing Liu

18 January 2021

Three-dimensional multicellular spheroids (MCSs) have received extensive attention in the field of biomedicine due to their ability to simulate the structure and function of tissues in vivo more accurately than traditional in vitro two-dimensional mo...

  • Article
  • Open Access
15 Citations
3,696 Views
11 Pages

Utilization of a Gas-Sensing System to Discriminate Smell and to Monitor Fermentation during the Manufacture of Oolong Tea Leaves

  • Ting-Shiang Tseng,
  • Mei-Hui Hsiao,
  • Po-An Chen,
  • Shu-Yen Lin,
  • Shih-Wen Chiu and
  • Da-Jeng Yao

17 January 2021

The operational duration of shaking tea leaves is a critical factor in the manufacture of oolong tea; this duration influences the formation of its flavor and fragrance. The current method to control the duration of fermentation relies on the olfacto...

  • Article
  • Open Access
15 Citations
3,196 Views
14 Pages

16 January 2021

Cavities are typical features in aeronautical structural parts and molds. For high-speed milling of multi-cavity parts, a reasonable processing sequence planning can significantly affect the machining accuracy and efficiency. This paper proposes an i...

  • Article
  • Open Access
4 Citations
4,301 Views
12 Pages

Sidewall Slope Control of InP Via Holes for 3D Integration

  • Jongwon Lee,
  • Kilsun Roh,
  • Sung-Kyu Lim and
  • Youngsu Kim

16 January 2021

This is the first demonstration of sidewall slope control of InP via holes with an etch depth of more than 10 μm for 3D integration. The process for the InP via holes utilizes a common SiO2 layer as an InP etch mask and conventional inductively co...

  • Article
  • Open Access
27 Citations
5,614 Views
13 Pages

3D-Printed Microfluidic Droplet Generator with Hydrophilic and Hydrophobic Polymers

  • Chandler A. Warr,
  • Hunter S. Hinnen,
  • Saroya Avery,
  • Rebecca J. Cate,
  • Gregory P. Nordin and
  • William G. Pitt

16 January 2021

Droplet generation has been widely used in conventional two-dimensional (2D) microfluidic devices, and has recently begun to be explored for 3D-printed droplet generators. A major challenge for 3D-printed devices is preventing water-in-oil droplets f...

  • Article
  • Open Access
6 Citations
4,485 Views
15 Pages

Long-Term Human Hematopoietic Stem Cell Culture in Microdroplets

  • Pilar Carreras,
  • Itziar González,
  • Miguel Gallardo,
  • Alejandra Ortiz-Ruiz,
  • Maria Luz Morales,
  • Jessica Encinas and
  • Joaquín Martínez-López

16 January 2021

We previously reported a new approach for micromanipulation and encapsulation of human stem cells using a droplet-based microfluidic device. This approach demonstrated the possibility of encapsulating and culturing difficult-to-preserve primary human...

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Micromachines - ISSN 2072-666X