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Article

Bovine Lactoferrin-Induced CCL1 Expression Involves Distinct Receptors in Monocyte-Derived Dendritic Cells and Their Monocyte Precursors

Istituto Superiore di Sanità, Department of Hematology, Oncology and Molecular Medicine, Viale Regina Elena 299, Rome 00161, Italy
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Dedicated to Patrizia, our precious and loved colleague and friend. Butterfly was her password and, like a butterfly, too soon she flew away. Her smile will always live in our hearts.
Academic Editor: Florian Lang
Toxins 2015, 7(12), 5472-5483; https://doi.org/10.3390/toxins7124897
Received: 18 November 2015 / Revised: 2 December 2015 / Accepted: 9 December 2015 / Published: 17 December 2015
(This article belongs to the Special Issue G-Protein Coupled Receptors as mediators of Toxin effects)
Lactoferrin (LF) exhibits a wide range of immunomodulatory activities including modulation of cytokine and chemokine secretion. In this study, we demonstrate that bovine LF (bLF) up-modulates, in a concentration- and time-dependent manner, CCL1 secretion in monocytes (Mo) at the early stage of differentiation toward dendritic cells (DCs), and in fully differentiated immature Mo-derived DCs (MoDCs). In both cell types, up-modulation of CCL1 secretion is an early event following bLF-mediated enhanced accumulation of CCL1 transcripts. Notably, bLF-mediated up-regulation of CCL1 involves the engagement of distinct surface receptors in MoDCs and their Mo precursors. We show that bLF-mediated engagement of CD36 contributes to CCL1 induction in differentiating Mo. Conversely, toll-like receptor (TLR)2 blocking markedly reduces bLF-induced CCL1 production in MoDCs. These findings add further evidence for cell-specific differential responses elicited by bLF through the engagement of distinct TLRs and surface receptors. Furthermore, the different responses observed at early and late stages of Mo differentiation towards DCs may be relevant in mediating bLF effects in specific body districts, where these cell types may be differently represented in physiopathological conditions. View Full-Text
Keywords: monocyte; dendritic cell; lactoferrin; CCL1; surface receptors monocyte; dendritic cell; lactoferrin; CCL1; surface receptors
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MDPI and ACS Style

Latorre, D.; Pulvirenti, N.; Covino, D.A.; Varano, B.; Purificato, C.; Rainaldi, G.; Gauzzi, M.C.; Fantuzzi, L.; Conti, L.; Donninelli, G.; Del Cornò, M.; Sabbatucci, M.; Gessani, S.; Puddu, P. Bovine Lactoferrin-Induced CCL1 Expression Involves Distinct Receptors in Monocyte-Derived Dendritic Cells and Their Monocyte Precursors. Toxins 2015, 7, 5472-5483. https://doi.org/10.3390/toxins7124897

AMA Style

Latorre D, Pulvirenti N, Covino DA, Varano B, Purificato C, Rainaldi G, Gauzzi MC, Fantuzzi L, Conti L, Donninelli G, Del Cornò M, Sabbatucci M, Gessani S, Puddu P. Bovine Lactoferrin-Induced CCL1 Expression Involves Distinct Receptors in Monocyte-Derived Dendritic Cells and Their Monocyte Precursors. Toxins. 2015; 7(12):5472-5483. https://doi.org/10.3390/toxins7124897

Chicago/Turabian Style

Latorre, Daniela, Nadia Pulvirenti, Daniela Angela Covino, Barbara Varano, Cristina Purificato, Gabriella Rainaldi, Maria Cristina Gauzzi, Laura Fantuzzi, Lucia Conti, Gloria Donninelli, Manuela Del Cornò, Michela Sabbatucci, Sandra Gessani, and Patrizia Puddu. 2015. "Bovine Lactoferrin-Induced CCL1 Expression Involves Distinct Receptors in Monocyte-Derived Dendritic Cells and Their Monocyte Precursors" Toxins 7, no. 12: 5472-5483. https://doi.org/10.3390/toxins7124897

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