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Open AccessArticle

Simultaneous Determination of Twenty Mycotoxins in the Korean Soybean Paste Doenjang by LC-MS/MS with Immunoaffinity Cleanup

Advanced Food Safety Research Group, BK21 Plus, School of Food Science and Technology, Chung-Ang University, Anseong 17546, Korea
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Toxins 2019, 11(10), 594; https://doi.org/10.3390/toxins11100594
Received: 17 September 2019 / Revised: 3 October 2019 / Accepted: 9 October 2019 / Published: 12 October 2019
(This article belongs to the Section Mycotoxins)
Doenjang, a Korean fermented soybean paste, is vulnerable to contamination by mycotoxins because it is directly exposed to environmental microbiota during fermentation. A method that simultaneously determines 20 mycotoxins in doenjang, including aflatoxins (AFs), ochratoxin A (OTA), zearalenone (ZEN), and fumonisins (FBs) with an immunoaffinity column cleanup was optimized and validated in doenjang using LC-MS/MS. The method showed good performance in the analysis of 20 mycotoxins in doenjang with good linearity (R2 > 0.999), intra- and inter-day precision (<16%), recovery (72–112%), matrix effect (87–104%), and measurement uncertainty (<42%). The validated method was applied to investigate mycotoxin contamination levels in commercial and homemade doenjang. The mycotoxins that frequently contaminated doenjang were AFs, OTA, ZEN, and FBs and the average contamination level and number of co-occurring mycotoxins in homemade doenjang were higher than those in commercially produced doenjang. View Full-Text
Keywords: mycotoxins; doenjang; simultaneous determination mycotoxins; doenjang; simultaneous determination
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MDPI and ACS Style

Woo, S.Y.; Ryu, S.Y.; Tian, F.; Lee, S.Y.; Park, S.B.; Chun, H.S. Simultaneous Determination of Twenty Mycotoxins in the Korean Soybean Paste Doenjang by LC-MS/MS with Immunoaffinity Cleanup. Toxins 2019, 11, 594.

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