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Article

Identification of the Red-Necked Longhorn Beetle Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae) with Real-Time PCR on Frass

1
Laboratory of Phytopathological Diagnostics and Molecular Biology, Plant Protection Service of Tuscany, 51100 Pistoia, Italy
2
ANSES Plant Health Laboratory, Entomology and Invasive Plants Unit, 34988 Montferrier-sur-Lez CEDEX, France
3
Department of Agricultural, Food and Agro-Environmental Sciences, University of Pisa, 56124 Pisa, Italy
4
National Research Council (CNR), Institute for Sustainable Plant Protection, 80055 Portici, Italy
5
Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy
6
Plant Protection Service of Campania, Centro Direzionale, 80124 Napoli, Italy
7
Phytopathological Laboratory, Campania Region, 80141 Napoli, Italy
*
Author to whom correspondence should be addressed.
Sustainability 2020, 12(15), 6041; https://doi.org/10.3390/su12156041
Received: 10 June 2020 / Revised: 21 July 2020 / Accepted: 24 July 2020 / Published: 28 July 2020
(This article belongs to the Special Issue Sustainable Defense Strategies for Pest Management)
Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae), the red-necked longhorn beetle is native to eastern Asia, where it is an important wood-borer of fruit and ornamental species of the genus Prunus. A. bungii is a quarantine pest in the European Union, following its accidental introduction and establishment in Germany and Italy, and is currently included in the list of priority pests. To confirm its infestations in outbreak areas, adult or larval specimens are needed to perform morphological or molecular analyses. The presence of A. bungii larvae inside the attacked trees makes the collection of specimens particularly difficult. Thus, we present two diagnostic protocols based on frass analysis with real-time PCR (probe and SYBR Green). The results obtained show that a non-invasive approach for detecting the presence of this harmful invasive pest can be a reliable and accurate alternative diagnostic tool in phytosanitary surveys, as well as to outline a sustainable pest management strategy. View Full-Text
Keywords: frass DNA; non-invasive diagnostic tool; phytosanitary survey; quarantine pest; xylophagous insect frass DNA; non-invasive diagnostic tool; phytosanitary survey; quarantine pest; xylophagous insect
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MDPI and ACS Style

Rizzo, D.; Taddei, A.; Da Lio, D.; Nugnes, F.; Barra, E.; Stefani, L.; Bartolini, L.; Griffo, R.V.; Spigno, P.; Cozzolino, L.; Rossi, E.; Garonna, A.P. Identification of the Red-Necked Longhorn Beetle Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae) with Real-Time PCR on Frass. Sustainability 2020, 12, 6041. https://doi.org/10.3390/su12156041

AMA Style

Rizzo D, Taddei A, Da Lio D, Nugnes F, Barra E, Stefani L, Bartolini L, Griffo RV, Spigno P, Cozzolino L, Rossi E, Garonna AP. Identification of the Red-Necked Longhorn Beetle Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae) with Real-Time PCR on Frass. Sustainability. 2020; 12(15):6041. https://doi.org/10.3390/su12156041

Chicago/Turabian Style

Rizzo, Domenico, Andrea Taddei, Daniele Da Lio, Francesco Nugnes, Eleonora Barra, Luciana Stefani, Linda Bartolini, Raffaele V. Griffo, Paola Spigno, Lucia Cozzolino, Elisabetta Rossi, and Antonio P. Garonna 2020. "Identification of the Red-Necked Longhorn Beetle Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae) with Real-Time PCR on Frass" Sustainability 12, no. 15: 6041. https://doi.org/10.3390/su12156041

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