Next Article in Journal
Decreased Threshold of Aggregation to Low-Dose Epinephrine is Evidence of Platelet Hyperaggregability in Patients with Thrombosis
Previous Article in Journal
Intensive Serial Biomarker Profiling for the Prediction of Neutropenic Fever in Patients with Hematologic Malignancies Undergoing Chemotherapy: A Pilot Study
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Hematology Reports
Volume 6
Issue 2
10.4081/hr.2014.5381
hematolrep-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
Hematology Reports is published by MDPI from Volume 14 Issue 1 (2022). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with PAGEPress.
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Multiparameter Flow Cytometry to Detect Hematogones and to Assess B-Lymphocyte Clonality in Bone Marrow Samples from Patients with Non-Hodgkin Lymphomas

by
Giovanni Carulli
1,*,
Virginia Ottaviano
1,
Valentina Guerri
1,
Stefano Giuntini
1,
Paola Sammuri
1,
Eugenio M. Ciancia
2 and
Antonio Azzarà
1
1
Division of Hematology, Department of Clinical and Experimental Medicine, Santa Chiara Hospital, University of Pisa, via Roma 67, 56126 Pisa, Italy
2
Second Division of Pathology, Pisa University Hospital, Pisa, Italy
*
Author to whom correspondence should be addressed.
Hematol. Rep. 2014, 6(2), 5381; https://doi.org/10.4081/hr.2014.5381
Submission received: 4 March 2014 / Revised: 25 May 2014 / Accepted: 3 June 2014 / Published: 26 June 2014

Abstract

Hematogones are precursors of B-lymphocytes detected in small numbers in the bone marrow. Flow cytometry is the most useful tool to identify hematogones and, so far, 4-color methods have been published. In addition, flow cytometry is used in the diagnosis and follow-up of lymphomas. We developed a flow cytometric 7-color method to enumerate hematogones and to assess B-lymphocyte clonality for routine purposes. We evaluated 171 cases of B-cell non-Hodgkin lymphomas, either at diagnosis or in the course of follow-up. By our diagnostic method, which was carried out by the combination K/λ/CD20/CD19/CD10/CD45/CD5, we were able to detect hematogones in 97.6% of samples and to distinguish normal B-lymphocytes, neoplastic lymphocytes and hematogones in a single step. The percentage of hematogones showed a significant inverse correlation with the degree of neoplastic infiltration and, when bone marrow samples not involved by disease were taken into consideration, resulted higher in patients during follow-up than in patients evaluated at diagnosis.
Keywords: B-lymphocytes; bone marrow; hematogones; multiparameter flow cytometry; non-Hodgkin lymphomas B-lymphocytes; bone marrow; hematogones; multiparameter flow cytometry; non-Hodgkin lymphomas

Share and Cite

MDPI and ACS Style

Carulli, G.; Ottaviano, V.; Guerri, V.; Giuntini, S.; Sammuri, P.; Ciancia, E.M.; Azzarà, A. Multiparameter Flow Cytometry to Detect Hematogones and to Assess B-Lymphocyte Clonality in Bone Marrow Samples from Patients with Non-Hodgkin Lymphomas. Hematol. Rep. 2014, 6, 5381. https://doi.org/10.4081/hr.2014.5381

AMA Style

Carulli G, Ottaviano V, Guerri V, Giuntini S, Sammuri P, Ciancia EM, Azzarà A. Multiparameter Flow Cytometry to Detect Hematogones and to Assess B-Lymphocyte Clonality in Bone Marrow Samples from Patients with Non-Hodgkin Lymphomas. Hematology Reports. 2014; 6(2):5381. https://doi.org/10.4081/hr.2014.5381

Chicago/Turabian Style

Carulli, Giovanni, Virginia Ottaviano, Valentina Guerri, Stefano Giuntini, Paola Sammuri, Eugenio M. Ciancia, and Antonio Azzarà. 2014. "Multiparameter Flow Cytometry to Detect Hematogones and to Assess B-Lymphocyte Clonality in Bone Marrow Samples from Patients with Non-Hodgkin Lymphomas" Hematology Reports 6, no. 2: 5381. https://doi.org/10.4081/hr.2014.5381

APA Style

Carulli, G., Ottaviano, V., Guerri, V., Giuntini, S., Sammuri, P., Ciancia, E. M., & Azzarà, A. (2014). Multiparameter Flow Cytometry to Detect Hematogones and to Assess B-Lymphocyte Clonality in Bone Marrow Samples from Patients with Non-Hodgkin Lymphomas. Hematology Reports, 6(2), 5381. https://doi.org/10.4081/hr.2014.5381

Article Metrics

Back to TopTop