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Article

Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio

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Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Ensenada 22860, Mexico
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Facultad de Ciencias de la Salud Unidad Valle de las Palmas, Universidad Autónoma de Baja California, Tijuana 22260, Mexico
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Programa de Maestría y Doctorado en Ciencias e Ingeniería, Facultad de Ciencias, Universidad Autónoma de Baja California, Ensenada 22860, Mexico
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Escuela de Ciencias de la Salud Unidad Valle Dorado, Universidad Autónoma de Baja California, Ensenada 22890, Mexico
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Research School of Chemistry & Applied Biomedical Sciences, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia
*
Authors to whom correspondence should be addressed.
Academic Editors: Sílvia Castro Coelho, Manuel A. N. Coelho, Fabio Pastorino and Christopher Scott
Pharmaceutics 2021, 13(7), 1000; https://doi.org/10.3390/pharmaceutics13071000
Received: 26 May 2021 / Revised: 23 June 2021 / Accepted: 28 June 2021 / Published: 1 July 2021
(This article belongs to the Special Issue Nanocarriers for Cancer Therapy and Diagnosis)
Silver nanoparticles (AgNPs) not only have shown remarkable results as antimicrobial and antiviral agents but also as antitumor agents. This work reports the complete characterization of five polyvinylpyrrolidone-coated AgNP (PVP-AgNP) formulations, their cytotoxic activity against human colon tumor cells (HCT-15), their cytotoxic effect on primary mouse cultures, and their lethal dose on BALB/c mice. The evaluated AgNP formulations have a composition within the ranges Ag: 1.14–1.32% w/w, PVP: 19.6–24.5% and H2O: 74.2–79.2% with predominant spherical shape within an average size range of 16–30 nm according to transmission electron microscopy (TEM). All formulations assessed increase mitochondrial ROS concentration and induce apoptosis as the leading death pathway on HCT-15 cells. Except for AgNP1, the growth inhibition potency of AgNP formulations of human colon tumor cancer cells (HCT-15) is 34.5 times higher than carboplatin, one of the first-line chemotherapy agents. Nevertheless, 5–10% of necrotic events, even at the lower concentration evaluated, were observed. The cytotoxic selectivity was confirmed by evaluating the cytotoxic effect on aorta, spleen, heart, liver, and kidney primary cultures from BALB/c mice. Despite the cytotoxic effects observed in vitro, the lethal dose and histopathological analysis showed the low toxicity of these formulations (all of them on Category 4 of the Globally Harmonized System of Classification and Labelling of Chemicals) and minor damage observed on analyzed organs. The results provide an additional example of the rational design of safety nanomaterials with antitumor potency and urge further experiments to complete the preclinical studies for these AgNP formulations. View Full-Text
Keywords: AgNPs; antitumor activity; colon cancer; HCT-15; cytotoxic selectivity; [metal]/[coating agent] ratio; therapeutic index; GSH classification AgNPs; antitumor activity; colon cancer; HCT-15; cytotoxic selectivity; [metal]/[coating agent] ratio; therapeutic index; GSH classification
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MDPI and ACS Style

Cruz-Ramírez, O.U.; Valenzuela-Salas, L.M.; Blanco-Salazar, A.; Rodríguez-Arenas, J.A.; Mier-Maldonado, P.A.; García-Ramos, J.C.; Bogdanchikova, N.; Pestryakov, A.; Toledano-Magaña, Y. Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio. Pharmaceutics 2021, 13, 1000. https://doi.org/10.3390/pharmaceutics13071000

AMA Style

Cruz-Ramírez OU, Valenzuela-Salas LM, Blanco-Salazar A, Rodríguez-Arenas JA, Mier-Maldonado PA, García-Ramos JC, Bogdanchikova N, Pestryakov A, Toledano-Magaña Y. Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio. Pharmaceutics. 2021; 13(7):1000. https://doi.org/10.3390/pharmaceutics13071000

Chicago/Turabian Style

Cruz-Ramírez, Omar Ulises, Lucía Margarita Valenzuela-Salas, Alberto Blanco-Salazar, José Antonio Rodríguez-Arenas, Paris A. Mier-Maldonado, Juan Carlos García-Ramos, Nina Bogdanchikova, Alexey Pestryakov, and Yanis Toledano-Magaña. 2021. "Antitumor Activity against Human Colorectal Adenocarcinoma of Silver Nanoparticles: Influence of [Ag]/[PVP] Ratio" Pharmaceutics 13, no. 7: 1000. https://doi.org/10.3390/pharmaceutics13071000

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