The Heterologous Expression of the p22 RNA Silencing Suppressor of the Crinivirus Tomato Chlorosis Virus from Tobacco Rattle Virus and Potato Virus X Enhances Disease Severity but Does Not Complement Suppressor-Defective Mutant Viruses
Yazmín Landeo-Ríos
Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”—Universidad de Málaga—Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental “La Mayora”, Algarrobo-Costa, 29750 Málaga, Spain
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Viruses 2017, 9(12), 358; https://doi.org/10.3390/v9120358
Received: 19 October 2017 / Revised: 9 November 2017 / Accepted: 22 November 2017 / Published: 24 November 2017
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
To counteract host antiviral RNA silencing, plant viruses express suppressor proteins that function as pathogenicity enhancers. The genome of the Tomato chlorosis virus (ToCV) (genus Crinivirus, family Closteroviridae) encodes an RNA silencing suppressor, the protein p22, that has been described as having one of the longest lasting local suppressor activities when assayed in Nicotiana benthamiana. Since suppression of RNA silencing and the ability to enhance disease severity are closely associated, we analyzed the effect of expressing p22 in heterologous viral contexts. Thus, we studied the effect of the expression of ToCV p22 from viral vectors Tobacco rattle virus (TRV) and Potato virus X (PVX), and from attenuated suppressor mutants in N. benthamiana plants. Our results show that although an exacerbation of disease symptoms leading to plant death was observed in the heterologous expression of ToCV p22 from both viruses, only in the case of TRV did increased viral accumulation occur. The heterologous expression of ToCV p22 could not complement suppressor-defective mutant viruses.
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Keywords:
Closteroviridae; Crinivirus; Tomato chlorosis virus; RNA silencing suppressor; virus pathogenicity
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MDPI and ACS Style
Landeo-Ríos, Y.; Navas-Castillo, J.; Moriones, E.; Cañizares, M.C. The Heterologous Expression of the p22 RNA Silencing Suppressor of the Crinivirus Tomato Chlorosis Virus from Tobacco Rattle Virus and Potato Virus X Enhances Disease Severity but Does Not Complement Suppressor-Defective Mutant Viruses. Viruses 2017, 9, 358.
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