An Aptamer-Based Proteomic Analysis of Plasma from Cats (Felis catus) with Clinical Feline Infectious Peritonitis

Curtis, Benjamin E.; Abdo, Zaid; Graham, Barbara; LaVoy, Alora; Evans, Samantha J. M.; Santangelo, Kelly; Dean, Gregg A.

doi:10.3390/v16010141

Open AccessArticle

An Aptamer-Based Proteomic Analysis of Plasma from Cats (Felis catus) with Clinical Feline Infectious Peritonitis

by

Benjamin E. Curtis

,

Zaid Abdo

,

Barbara Graham

,

Alora LaVoy

,

Samantha J. M. Evans

,

Kelly Santangelo

and

Gregg A. Dean

^*

Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523, USA

^*

Author to whom correspondence should be addressed.

Viruses 2024, 16(1), 141; https://doi.org/10.3390/v16010141

Submission received: 17 October 2023 / Revised: 13 January 2024 / Accepted: 16 January 2024 / Published: 18 January 2024

(This article belongs to the Special Issue Viral Infections in Companion Animals: Volume 2)

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Abstract

Feline infectious peritonitis (FIP) is a systemic disease manifestation of feline coronavirus (FCoV) and is the most important cause of infectious disease-related deaths in domestic cats. FIP has a variable clinical manifestation but is most often characterized by widespread vasculitis with visceral involvement and/or neurological disease that is typically fatal in the absence of antiviral therapy. Using an aptamer-based proteomics assay, we analyzed the plasma protein profiles of cats who were naturally infected with FIP (n = 19) in comparison to the plasma protein profiles of cats who were clinically healthy and negative for FCoV (n = 17) and cats who were positive for the enteric form of FCoV (n = 9). We identified 442 proteins that were significantly differentiable; in total, 219 increased and 223 decreased in FIP plasma versus clinically healthy cat plasma. Pathway enrichment and associated analyses showed that differentiable proteins were related to immune system processes, including the innate immune response, cytokine signaling, and antigen presentation, as well as apoptosis and vascular integrity. The relevance of these findings is discussed in the context of previous studies. While these results have the potential to inform diagnostic, therapeutic, and preventative investigations, they represent only a first step, and will require further validation.

Keywords: feline infectious peritonitis; FIP; protein profile; proteomics; pathway analysis; enrichment

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MDPI and ACS Style

Curtis, B.E.; Abdo, Z.; Graham, B.; LaVoy, A.; Evans, S.J.M.; Santangelo, K.; Dean, G.A. An Aptamer-Based Proteomic Analysis of Plasma from Cats (Felis catus) with Clinical Feline Infectious Peritonitis. Viruses 2024, 16, 141. https://doi.org/10.3390/v16010141

AMA Style

Curtis BE, Abdo Z, Graham B, LaVoy A, Evans SJM, Santangelo K, Dean GA. An Aptamer-Based Proteomic Analysis of Plasma from Cats (Felis catus) with Clinical Feline Infectious Peritonitis. Viruses. 2024; 16(1):141. https://doi.org/10.3390/v16010141

Chicago/Turabian Style

Curtis, Benjamin E., Zaid Abdo, Barbara Graham, Alora LaVoy, Samantha J. M. Evans, Kelly Santangelo, and Gregg A. Dean. 2024. "An Aptamer-Based Proteomic Analysis of Plasma from Cats (Felis catus) with Clinical Feline Infectious Peritonitis" Viruses 16, no. 1: 141. https://doi.org/10.3390/v16010141

APA Style

Curtis, B. E., Abdo, Z., Graham, B., LaVoy, A., Evans, S. J. M., Santangelo, K., & Dean, G. A. (2024). An Aptamer-Based Proteomic Analysis of Plasma from Cats (Felis catus) with Clinical Feline Infectious Peritonitis. Viruses, 16(1), 141. https://doi.org/10.3390/v16010141

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An Aptamer-Based Proteomic Analysis of Plasma from Cats (Felis catus) with Clinical Feline Infectious Peritonitis

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