Colonized Sabethes cyaneus, a Sylvatic New World Mosquito Species, Shows a Low Vector Competence for Zika Virus Relative to Aedes aegypti
Abstract
:1. Introduction
2. Materials and Methods
2.1. Virus Strains and Cell Lines
2.2. Mosquito Strains
2.3. Mouse Infections and Mosquito Feeding
2.4. Mosquito Incubation and Virus Quantification
3. Results
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Mosquito Day Post-Feeding | Mouse Day Post-Infection | ZIKV Titer (log10 pfu/mL) | N | No. (%) Infection | No. (%) Dissemination | No. (%) Transmission |
---|---|---|---|---|---|---|
3 | 1 | 5.38 | 5 | 0 | 0 | 0 |
4 | 1 | 5.38 | 5 | 0 | 0 | 0 |
5 | 1 | 5.38 | 5 | 0 | 0 | 0 |
7 | 1 | 5.38 | 5 | 0 | 0 | 0 |
14 | 1 | 5.38 | 5 | 0 | 0 | 0 |
3 | 2 | 6.83 | 7 | 0 | 0 | 0 |
4 | 2 | 6.83 | 7 | 0 | 0 | 0 |
5 | 2 | 6.83 | 7 | 0 | 0 | 0 |
7 | 2 | 6.83 | 7 | 0 | 0 | 0 |
14 | 2 | 6.83 | 7 | 0 | 0 | 0 |
21 | 2 | 6.83 | 9 | 1 (11.1) | 1 (11.1) | 1 (11.1) |
Mosquito Day Post-Feeding | Mouse Day Post-Infection | ZIKV Titer (log10 pfu/mL) | N | No. (%) Infection | No. (%) Dissemination | No. (%) Transmission |
---|---|---|---|---|---|---|
14 | 1 | 5.38 | 10 | 3 (30) | 1 (10) | 0 (0) |
21 | 1 | 5.38 | 10 | 4 (40) | 2 (20) | 2 (20) |
14 | 2 | 6.83 | 10 | 10 (100) | 10 (100) | 7 (70) |
21 | 2 | 6.83 | 10 | 10 (100) | 10 (100) | 7 (70) |
Species | Mosquito Day Post Feeding | Mouse Day Post Infection | Mean Body Titer * (log10 pfu/mL) ± 1SE (N) | Mean Legs Titer * (log10 pfu/mL) ± 1SE (N) | Mean Saliva Titer * (log10 pfu/mL) ± 1SE (N) |
---|---|---|---|---|---|
Sabethes cyaneus | 21 | 2 | 5.4 (1) | 4.4 (1) | 1.1 (1) |
Aedes aegypti | 14 | 1 | 4.5 ± 0.2 (3) | 3.2 (1) | NA (0) |
21 | 1 | 5.1 ± 0.2 (4) | 4.4 ± 0.4 (2) | 2.8 ± 1.0 (2) | |
14 | 2 | 5.2 ± 0.2 (10) | 4.4 ± 0.4 (10) | 2.8 ± 0.3 (7) | |
21 | 2 | 5.7 ± 0.1 (10) | 4.7 ± 0.3 (10) | 2.9 ± 0.5 (7) |
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Karna, A.K.; Azar, S.R.; Plante, J.A.; Yun, R.; Vasilakis, N.; Weaver, S.C.; Hansen, I.A.; Hanley, K.A. Colonized Sabethes cyaneus, a Sylvatic New World Mosquito Species, Shows a Low Vector Competence for Zika Virus Relative to Aedes aegypti. Viruses 2018, 10, 434. https://doi.org/10.3390/v10080434
Karna AK, Azar SR, Plante JA, Yun R, Vasilakis N, Weaver SC, Hansen IA, Hanley KA. Colonized Sabethes cyaneus, a Sylvatic New World Mosquito Species, Shows a Low Vector Competence for Zika Virus Relative to Aedes aegypti. Viruses. 2018; 10(8):434. https://doi.org/10.3390/v10080434
Chicago/Turabian StyleKarna, Ajit K., Sasha R. Azar, Jessica A. Plante, Rumei Yun, Nikos Vasilakis, Scott C. Weaver, Immo A. Hansen, and Kathryn A. Hanley. 2018. "Colonized Sabethes cyaneus, a Sylvatic New World Mosquito Species, Shows a Low Vector Competence for Zika Virus Relative to Aedes aegypti" Viruses 10, no. 8: 434. https://doi.org/10.3390/v10080434