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Article

Inhibition of miR-141 and miR-200a Increase DLC-1 and ZEB2 Expression, Enhance Migration and Invasion in Metastatic Serous Ovarian Cancer

1
Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur 56000, Malaysia
2
Department of Medicine, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur 56000, Malaysia
*
Author to whom correspondence should be addressed.
Int. J. Environ. Res. Public Health 2020, 17(8), 2766; https://doi.org/10.3390/ijerph17082766
Received: 6 March 2020 / Revised: 2 April 2020 / Accepted: 4 April 2020 / Published: 17 April 2020
(This article belongs to the Special Issue Gynecological Cancer)
The role of microRNA (miRNA) in ovarian cancer has been extensively studied as a regulator for its targeted genes. However, its specific role in metastatic serous ovarian cancer (SOC) is yet to be explored. This paper aims to investigate the differentially expressed miRNAs in metastatic SOC compared to normal. Locked nucleic acid PCR was performed to profile miRNA expression in 11 snap frozen metastatic SOC and 13 normal ovarian tissues. Functional analysis and regulation of their targeted genes were assessed in vitro. Forty-eight miRNAs were significantly differentially expressed in metastatic SOC as compared to normal. MiR-19a is a novel miRNA to be upregulated in metastatic SOC compared to normal. DLC1 is possibly regulated by miR-141 in SOC. MiR-141 inhibition led to significantly reduced cell viability. Cell migration and invasion were significantly increased following miRNA inhibition. This study showed the aberrantly expressed miRNAs in metastatic SOC and the roles of miRNAs in the regulation of their targeted genes and ovarian carcinogenesis. View Full-Text
Keywords: serous ovarian cancer; metastasis; microRNA; MIRN141 microRNA; MIRN200 microRNA serous ovarian cancer; metastasis; microRNA; MIRN141 microRNA; MIRN200 microRNA
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MDPI and ACS Style

Wahab, N.A.; Othman, Z.; Nasri, N.W.M.; Mokhtar, M.H.; Ibrahim, S.F.; Hamid, A.A.; Raja Ali, R.A.; Mokhtar, N.M. Inhibition of miR-141 and miR-200a Increase DLC-1 and ZEB2 Expression, Enhance Migration and Invasion in Metastatic Serous Ovarian Cancer. Int. J. Environ. Res. Public Health 2020, 17, 2766. https://doi.org/10.3390/ijerph17082766

AMA Style

Wahab NA, Othman Z, Nasri NWM, Mokhtar MH, Ibrahim SF, Hamid AA, Raja Ali RA, Mokhtar NM. Inhibition of miR-141 and miR-200a Increase DLC-1 and ZEB2 Expression, Enhance Migration and Invasion in Metastatic Serous Ovarian Cancer. International Journal of Environmental Research and Public Health. 2020; 17(8):2766. https://doi.org/10.3390/ijerph17082766

Chicago/Turabian Style

Wahab, Norhazlina A., Zahreena Othman, Noor W.M. Nasri, Mohd H. Mokhtar, Siti F. Ibrahim, Adila A. Hamid, Raja A. Raja Ali, and Norfilza M. Mokhtar. 2020. "Inhibition of miR-141 and miR-200a Increase DLC-1 and ZEB2 Expression, Enhance Migration and Invasion in Metastatic Serous Ovarian Cancer" International Journal of Environmental Research and Public Health 17, no. 8: 2766. https://doi.org/10.3390/ijerph17082766

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