Biochemical Studies of the Lagunamides, Potent Cytotoxic Cyclic Depsipeptides from the Marine Cyanobacterium Lyngbya majuscula
Abstract
:1. Introduction
2. Results and Discussion
2.1. Structures of Lagunamides and the Aurilide-Class of Molecules
2.2. Evaluation of Lagunamide A against BJ and BJ shp53 Cell Lines
Cell Lines | Cell Types | 1 | 2 | 3 a | 4 b | 5 b | 6 c |
---|---|---|---|---|---|---|---|
BJ | Foreskin fibroblast | 20.2 | |||||
BJ shp53 | p53 knocked down fibroblast | 58.8 | |||||
P388 | Murine leukemia | 6.4 | 20.5 | 4.2 | |||
A549 | Lung adenocarcinoma epithelial | 2.9 | |||||
NCI-H460 | Lung large-cell carcinoma | 10 | 50 | ||||
PC3 | Prostate carcinoma | 2.5 | |||||
HCT8 | Ileocecal colorectal adenocarcinoma | 1.6 | 5.2 | ||||
SK-OV3 | Ovarian carcinoma | 3.8 | 7.5 | ||||
HeLaS3 | Human epithelial carcinoma | 11 | |||||
MDA-MB-435 | Human breast carcinoma | 14.6 | |||||
A10 | Vascular smooth muscle | 59.1 | |||||
Neuro-2a | Mouse neuroblastoma | 40 | 130 |
2.3. Evaluation of Lagunamide A against a Panel of Cancer Cell Lines
2.4. Biochemical Studies of Lagunamide A Using HCT8 and MCF7 Cell Lines
3. Experimental Section
3.1. Extraction and Isolation of Lagunamides A and B
3.2. Cancer Cell Lines and Media
3.3. MTT Cytotoxicity Assay
3.4. Western Blot Analysis
4. Conclusions
Acknowledgments
- Samples Availability: Available from the authors.
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Tripathi, A.; Fang, W.; Leong, D.T.; Tan, L.T. Biochemical Studies of the Lagunamides, Potent Cytotoxic Cyclic Depsipeptides from the Marine Cyanobacterium Lyngbya majuscula. Mar. Drugs 2012, 10, 1126-1137. https://doi.org/10.3390/md10051126
Tripathi A, Fang W, Leong DT, Tan LT. Biochemical Studies of the Lagunamides, Potent Cytotoxic Cyclic Depsipeptides from the Marine Cyanobacterium Lyngbya majuscula. Marine Drugs. 2012; 10(5):1126-1137. https://doi.org/10.3390/md10051126
Chicago/Turabian StyleTripathi, Ashootosh, Wanru Fang, David Tai Leong, and Lik Tong Tan. 2012. "Biochemical Studies of the Lagunamides, Potent Cytotoxic Cyclic Depsipeptides from the Marine Cyanobacterium Lyngbya majuscula" Marine Drugs 10, no. 5: 1126-1137. https://doi.org/10.3390/md10051126