Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma
Abstract
:1. Introduction
2. Experimental Section
2.1. Materials
2.2. Immobilization of FITC-labeled protein A
2.3. Fluorescence intensity measurement
3. Results and Discussion
3.1. Fluorescence spectrum of FITC-protein A immobilized glass
3.2. Dependency of fluorescence enhancement on IgG concentration
4. Conclusions
Acknowledgments
References and Notes
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Ogawa, T.; Aoyagi, S.; Miyasaka, T.; Sakai, K. Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma. Sensors 2009, 9, 8271-8277. https://doi.org/10.3390/s91008271
Ogawa T, Aoyagi S, Miyasaka T, Sakai K. Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma. Sensors. 2009; 9(10):8271-8277. https://doi.org/10.3390/s91008271
Chicago/Turabian StyleOgawa, Takehito, Satoka Aoyagi, Takehiro Miyasaka, and Kiyotaka Sakai. 2009. "Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma" Sensors 9, no. 10: 8271-8277. https://doi.org/10.3390/s91008271
APA StyleOgawa, T., Aoyagi, S., Miyasaka, T., & Sakai, K. (2009). Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma. Sensors, 9(10), 8271-8277. https://doi.org/10.3390/s91008271