Sensors 2009, 9(10), 7694-7710; https://doi.org/10.3390/s91007694
A Portable Luminometer with a Disposable Electrochemiluminescent Biosensor for Lactate Determination
Antonio Martínez-Olmos 1, Julio Ballesta-Claver 2, Alberto J. Palma 1
, Maria del Carmen Valencia-Mirón 2 and Luis Fermin Capitán-Vallvey 2,*
, Maria del Carmen Valencia-Mirón 2 and Luis Fermin Capitán-Vallvey 2,*
1
Ecsens, Department of Electronics and Computer Technology, Granada, Spain
2
ECsens, Department of Analytical Chemistry, Campus Fuentenueva, Faculty of Sciences, University of Granada, Spain
*
Author to whom correspondence should be addressed.
Received: 16 July 2009 / Revised: 8 September 2009 / Accepted: 17 September 2009 / Published: 28 September 2009
(This article belongs to the Section Biosensors)
Abstract
A hand-held luminometer for measuring electrochemiluminescence (ECL) for lactate determination and based on one-shot biosensors fabricated using screen-printed electrodes is described. The lactate recognition system is based on lactate oxidase and the transduction system consists of electro-oxidation of luminol, with all the reagents immobilized in a Methocel membrane. The membrane composition and reaction conditions have been optimized to obtain adequate sensitivity. The luminometer is based on a large silicon photodiode as detector and includes a programmable potentiostat to initialize the chemical reaction and signal processing circuitry, designed to acquire a low level photocurrent with offset cancelation, low pass filtering for noise attenuation and adjustable gain up to 1012 V/A. The one-shot biosensor responds to lactate rapidly, with an acquisition time of 2.5 min, obtaining a linear dependence from 8 × 10−6 to 2 × 10−4 M, a detection limit of 2.4 × 10−6 M and a sensor-to-sensor reproducibility (relative standard deviation, RSD) of around 7–10 % at the medium level of the range. View Full-TextKeywords:
portable instrument; microcontroller; electrochemiluminescence measurement; disposable biosensor; lactate determination
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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MDPI and ACS Style
Martínez-Olmos, A.; Ballesta-Claver, J.; Palma, A.J.; Valencia-Mirón, M.C.; Capitán-Vallvey, L.F. A Portable Luminometer with a Disposable Electrochemiluminescent Biosensor for Lactate Determination. Sensors 2009, 9, 7694-7710.