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Sensors
Volume 21
Issue 14
10.3390/s21144902
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Communication

Highly Sensitive Electrochemical Sensor for Diagnosis of Diabetic Ketoacidosis (DKA) by Measuring Ketone Bodies in Urine

by
Anna Go
1,†,
Sung Ryul Park
1,†,
Yejin Ku
2,
Mingge Sun
1,
Sangho Yeon
1,
Jin-Kyun Lee
2,
Sang Wook Lee
3,4,* and
Min-Ho Lee
1,*
1
School of Integrative Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Korea
2
Program in Environmental and Polymer Engineering, Department of Polymer Science and Engineering, Inha University, 100 Inha-Ro, Nam-Gu, Incheon 22212, Korea
3
Bio-Health Product Research Center, Inje University, Gimhae-si 50834, Korea
4
PCL Inc., Star Valley, 99, Digital-ro-9-gil, Geumcheon-gu, Seoul 08510, Korea
*
Authors to whom correspondence should be addressed.
†
Equal contribution.
Sensors 2021, 21(14), 4902; https://doi.org/10.3390/s21144902
Submission received: 1 June 2021 / Revised: 7 July 2021 / Accepted: 14 July 2021 / Published: 19 July 2021
(This article belongs to the Special Issue Recent Advances in Immunosensors and Biosensors)
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Abstract

In this report, we present an enzyme deposited Au electrode for an electrochemical measurement of acetylacetic acid (AcAc) in urine. The electrode has an immobilized layer of a mixture of D-β-hydroxybutyrate dehydrogenase (HBDH) and nicotinamide adenine dinucleotide (NADH) as sensing material to investigate its electroanalytical properties by means of cyclic voltammetry (CV). The modified electrodes are used for the detection of AcAc and present a linear current increase when the AcAc concentration increases. The electrode presents a limit of detection (LOD) of 6.25 mg/dL in the range of 6.25–100 mg/dL for investigation of clinical relevance. Finally, the electrode was evaluated using 20 patient samples. The measured results of urine ketone by the developed electrode were compared with the clinical results from a commercial kit, and the analysis showed good agreement. The proposed electrode was demonstrated to be a very promising platform as a miniaturized electrochemical analyzer for point-of-care monitoring of the critical biochemical parameters such as urine ketone.
Keywords: ketone body; electrochemical sensor; cyclic voltammetry; non-invasive detection; point of care technology ketone body; electrochemical sensor; cyclic voltammetry; non-invasive detection; point of care technology

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MDPI and ACS Style

Go, A.; Park, S.R.; Ku, Y.; Sun, M.; Yeon, S.; Lee, J.-K.; Lee, S.W.; Lee, M.-H. Highly Sensitive Electrochemical Sensor for Diagnosis of Diabetic Ketoacidosis (DKA) by Measuring Ketone Bodies in Urine. Sensors 2021, 21, 4902. https://doi.org/10.3390/s21144902

AMA Style

Go A, Park SR, Ku Y, Sun M, Yeon S, Lee J-K, Lee SW, Lee M-H. Highly Sensitive Electrochemical Sensor for Diagnosis of Diabetic Ketoacidosis (DKA) by Measuring Ketone Bodies in Urine. Sensors. 2021; 21(14):4902. https://doi.org/10.3390/s21144902

Chicago/Turabian Style

Go, Anna, Sung Ryul Park, Yejin Ku, Mingge Sun, Sangho Yeon, Jin-Kyun Lee, Sang Wook Lee, and Min-Ho Lee. 2021. "Highly Sensitive Electrochemical Sensor for Diagnosis of Diabetic Ketoacidosis (DKA) by Measuring Ketone Bodies in Urine" Sensors 21, no. 14: 4902. https://doi.org/10.3390/s21144902

APA Style

Go, A., Park, S. R., Ku, Y., Sun, M., Yeon, S., Lee, J.-K., Lee, S. W., & Lee, M.-H. (2021). Highly Sensitive Electrochemical Sensor for Diagnosis of Diabetic Ketoacidosis (DKA) by Measuring Ketone Bodies in Urine. Sensors, 21(14), 4902. https://doi.org/10.3390/s21144902

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