AniScan Using Extracellular Cyclic AMP-Dependent Protein Kinase A as a Serum Biomarker Assay for the Diagnosis of Malignant Tumors in Dogs
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Samples
2.2. Diagnosis of Malignant and Benign Tumors
2.3. Assessment of Serum ECPKA Autoantibody
2.4. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Malignant Tumor | Benign Tumor | Non-Tumor Disease | Healthy | |
---|---|---|---|---|
Number | 227 | 60 | 79 | 184 |
Median age (Range) | 11 (1–17) | 10 (4–17) | 8 (1–15) | 5 (0.5–13) |
Sex (n) | F (33), M (16), NF (90), NM (88) | F (7), M (5), NF (13), NM (35) | F (10), M (3), NF (17), NM (49) | F (46), M (42), NF (57), NM (39) |
Breed (n) | Maltese (48), Shih tzu (25), Yorkshire Terrier (22), Poodle (21), Cocker Spaniel (15), Retriever (13), Schnauzer (11), Pomeranian (10), French Bulldog (3), other (59) | Maltese (10), Shih tzu (8), Cocker Spaniel (7), Beagle (5), Poodle (6), Bichon Frise (2), other (22) | Maltese (20), Yorkshire Terrier (8), Poodle (7), Pomeranian (6), Shih tzu (3), Cocker Spaniel (3), other (32) | Beagle (54), Maltese (29), Shih tzu (26), Pomeranian (11), Yorkshire Terrier (9), Bichon Frise (4), other (51) |
Type of Non-Tumor Disease | Number (n) |
---|---|
Orthopedic | 19 |
Dermatologic | 17 |
Gastrointestinal | 11 |
Hepatobiliary | 6 |
Cardiovascular | 6 |
Urologic | 5 |
Endocrine | 3 |
Immune-mediated | 3 |
Ophthalmologic | 3 |
Neurologic | 2 |
Other | 4 |
Value | |
---|---|
AUROC | 0.818 |
95% CI | 0.777–0.853 |
Standard error | 0.019 |
Sensitivity | 81.08% |
Specificity | 87.02% |
Accuracy | 84.67% |
PPV | 80.35 |
NPV | 87.53 |
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Lee, J.-E.; Song, W.-J.; Lee, H.; Kim, B.-G.; Kim, T.; Lee, C.; Jang, B.; Youn, H.-Y.; Choi, U.-S.; Bhang, D.-H. AniScan Using Extracellular Cyclic AMP-Dependent Protein Kinase A as a Serum Biomarker Assay for the Diagnosis of Malignant Tumors in Dogs. Sensors 2020, 20, 4075. https://doi.org/10.3390/s20154075
Lee J-E, Song W-J, Lee H, Kim B-G, Kim T, Lee C, Jang B, Youn H-Y, Choi U-S, Bhang D-H. AniScan Using Extracellular Cyclic AMP-Dependent Protein Kinase A as a Serum Biomarker Assay for the Diagnosis of Malignant Tumors in Dogs. Sensors. 2020; 20(15):4075. https://doi.org/10.3390/s20154075
Chicago/Turabian StyleLee, Ji-Eun, Woo-Jin Song, Hunjoo Lee, Byung-Gak Kim, Taeho Kim, Changsun Lee, Bonghwan Jang, Hwa-Young Youn, Ul-Soo Choi, and Dong-Ha Bhang. 2020. "AniScan Using Extracellular Cyclic AMP-Dependent Protein Kinase A as a Serum Biomarker Assay for the Diagnosis of Malignant Tumors in Dogs" Sensors 20, no. 15: 4075. https://doi.org/10.3390/s20154075
APA StyleLee, J.-E., Song, W.-J., Lee, H., Kim, B.-G., Kim, T., Lee, C., Jang, B., Youn, H.-Y., Choi, U.-S., & Bhang, D.-H. (2020). AniScan Using Extracellular Cyclic AMP-Dependent Protein Kinase A as a Serum Biomarker Assay for the Diagnosis of Malignant Tumors in Dogs. Sensors, 20(15), 4075. https://doi.org/10.3390/s20154075